Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,806,158 T→C 73.8% intergenic (‑75/‑212) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,1580TC73.8% 229.7 / 76.6 140intergenic (‑75/‑212)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (20/16);  new base C (54/47);  total (74/66)
Fisher's exact test for biased strand distribution p-value = 8.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

ACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTT  >  NC_000913/1806064‑1806253
                                                                                              |                                                                                               
aCTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTg                                                                                            <  2:3148323/100‑1 (MQ=255)
   gTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGaa                                                                                         >  2:4082377/1‑100 (MQ=255)
   gTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTg                                                                                            >  2:1089402/1‑97 (MQ=255)
    tCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGAGGTCCCGGAAACTTTCATAAGGACCAAAGATTAGCGTCCCTGGaaa                                                                                        >  2:3400779/1‑100 (MQ=255)
     cTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAg                                                                                       >  2:348042/1‑100 (MQ=255)
     cTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAg                                                                                       <  1:1904604/100‑1 (MQ=255)
     cTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAg                                                                                       >  1:296758/1‑100 (MQ=255)
      ttCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAgg                                                                                      <  2:2034123/100‑1 (MQ=255)
       tCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGt                                                                                     >  2:3486551/1‑100 (MQ=255)
       tCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGt                                                                                     >  2:1394471/1‑100 (MQ=255)
       tCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGt                                                                                     >  1:2954422/1‑100 (MQ=255)
        cAAAAGCTTCATTTATCAAGGGTCCGTACAACAAAAAAAGAGACCATCGCGGTCACGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTa                                                                                    >  2:4086362/1‑100 (MQ=255)
        cAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTa                                                                                    <  1:1789647/100‑1 (MQ=255)
          aaaGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAAc                                                                                  >  1:3948844/1‑100 (MQ=255)
          aaaGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAAc                                                                                  >  1:2141329/1‑100 (MQ=255)
           aaGCTTCATTTATCAAGAGTCCGTAAAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACg                                                                                 <  2:3232570/100‑1 (MQ=255)
             gCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGaa                                                                               >  1:2255675/1‑100 (MQ=255)
              cTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAAt                                                                              >  2:2096774/1‑100 (MQ=255)
                tCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCGTCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATta                                                                            >  2:2221515/1‑100 (MQ=255)
                 cATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATtat                                                                           >  2:169231/1‑100 (MQ=255)
                 cATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATtat                                                                           <  1:4141934/100‑1 (MQ=255)
                  aTTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCACGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATtata                                                                          >  1:1221426/1‑100 (MQ=255)
                   tttATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATaa                                                                         >  1:3199718/1‑100 (MQ=255)
                      aTCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATaaaaa                                                                      >  2:1648032/1‑100 (MQ=255)
                      aTCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATaaaaa                                                                      <  2:205169/100‑1 (MQ=255)
                      aTCAAGAGACAGAACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATaaaaa                                                                      <  1:1033826/100‑1 (MQ=255)
                       tCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAg                                                                     >  1:3823629/1‑100 (MQ=255)
                        cAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAgg                                                                    >  2:698375/1‑100 (MQ=255)
                        cAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAgg                                                                    >  2:2627800/1‑100 (MQ=255)
                         aaGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGgc                                                                   >  1:2442514/1‑100 (MQ=255)
                           gagTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGgcgc                                                                 >  1:2066287/1‑100 (MQ=255)
                            agTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGcgcg                                                                <  2:619056/100‑1 (MQ=255)
                             gTCCGTACAACAAAAAAAGAGACCAGCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTCTAAAAAGGcgcg                                                                <  1:2997091/99‑1 (MQ=255)
                              tCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGaa                                                              <  1:2387566/100‑1 (MQ=255)
                                cGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATa                                                            <  1:3826724/100‑1 (MQ=255)
                                  tACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAAc                                                          >  2:2453415/1‑100 (MQ=255)
                                   acaacaAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCACTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACt                                                         <  2:1523754/100‑1 (MQ=255)
                                   acaacaAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACt                                                         >  2:1622532/1‑100 (MQ=255)
                                     aacaacAAAAGAGA‑CATCGCGGTCCCGGAAACTATCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAg                                                      <  2:2869206/100‑1 (MQ=255)
                                     aacaaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACtt                                                        <  2:1165818/99‑1 (MQ=255)
                                      acaaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAg                                                      >  2:148507/1‑100 (MQ=255)
                                      acaaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAg                                                      >  2:412919/1‑100 (MQ=255)
                                      acaaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGAGAATAACTTAg                                                      >  2:2477354/1‑100 (MQ=255)
                                       caaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGc                                                     <  1:392877/100‑1 (MQ=255)
                                       caaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGc                                                     >  1:2250590/1‑100 (MQ=255)
                                       caaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGc                                                     >  2:3436956/1‑100 (MQ=255)
                                       caaAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGc                                                     <  1:1642449/100‑1 (MQ=255)
                                         aaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCaa                                                   >  1:1240901/1‑100 (MQ=255)
                                         aaaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCaa                                                   <  1:3378617/100‑1 (MQ=255)
                                          aaaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAAt                                                  >  2:3580202/1‑100 (MQ=255)
                                           aaaaGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATg                                                 <  2:3684866/100‑1 (MQ=255)
                                              agagACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTAt                                              <  2:3679075/100‑1 (MQ=255)
                                               gagaCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTAtt                                             <  1:2610744/100‑1 (MQ=255)
                                                agaCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTc                                            <  1:4114877/100‑1 (MQ=255)
                                                 gaCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCt                                           >  1:3298488/1‑100 (MQ=255)
                                                 gaCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCt                                           <  2:2822863/100‑1 (MQ=255)
                                                  aCCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCtt                                          >  1:3668751/1‑100 (MQ=255)
                                                   ccATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTa                                         >  1:1473891/1‑100 (MQ=255)
                                                   ccATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTa                                         <  2:2250576/100‑1 (MQ=255)
                                                   ccATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTa                                         >  1:1688599/1‑100 (MQ=255)
                                                    cATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTa                                         <  2:3854476/99‑1 (MQ=255)
                                                    cATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTAt                                        >  2:1600484/1‑100 (MQ=255)
                                                      tCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTAttt                                      >  1:1378743/1‑100 (MQ=255)
                                                        gcgGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCa                                    >  2:3073123/1‑100 (MQ=255)
                                                         cgGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAt                                   <  2:720389/100‑1 (MQ=255)
                                                           gTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttt                                 <  1:2435101/100‑1 (MQ=255)
                                                            tCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAtttt                                <  1:2627815/100‑1 (MQ=255)
                                                             cccGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt                               >  1:3232766/1‑100 (MQ=255)
                                                             cccGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt                               >  1:1396310/1‑100 (MQ=255)
                                                             cccGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt                               >  1:3359045/1‑100 (MQ=255)
                                                             cccGGAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCAttttt                               >  1:3871590/1‑100 (MQ=255)
                                                               cGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTg                             <  2:513360/100‑1 (MQ=255)
                                                               cGGAAACTTGCTTAAGGATCAAAGATTAGCGCCCCTGGAGAGTTAACGAATTATAAAAAGGCTCGAATCACTTAGGGATGTATTCTTATTTCAtctat                               <  2:2862322/98‑5 (MQ=255)
                                                                ggAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGa                            >  2:1145907/1‑100 (MQ=255)
                                                                 gAAACTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGaa                           <  2:3992788/100‑1 (MQ=255)
                                                                  aaaCTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGTGAATAACTTAGCAATGTATTCTTATTTCAGTTTTTGAAt                          <  2:2522125/100‑1 (MQ=255)
                                                                  aaaCTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAAt                          <  2:3948209/100‑1 (MQ=255)
                                                                  aaaCTTACTTAAATATCGAAAATTAGCGTGCCTAGCAAGGTAAAGAATTATAAATCGTCGCGAAGCACTTTGCAATGGATATGTATTTCATTTTTTg                             <  2:3846754/97‑1 (MQ=255)
                                                                     cagtCTTACGGATCAAACACTCGCGTCCCTGGCAAGGTAACGAATTATAAAAAGGCGCGCCTAACTTAGCAATGTATTCTTATTACAattttttcat                          >  2:127812/4‑93 (MQ=255)
                                                                     cTTTCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAg                       >  2:4004924/1‑100 (MQ=255)
                                                                       ttCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCa                     <  2:3237559/100‑1 (MQ=255)
                                                                       ttCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCa                     <  1:1394479/100‑1 (MQ=255)
                                                                        tCTTAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAAATTAGCAATGTATTCTTATTTCATTTTTTGAATAATCAt                    >  2:2304783/1‑100 (MQ=255)
                                                                         ctgaaGGATCAAAGATGAGCGGCCCTGGAAATGTTATGAATTTTAAAAAGGCTTGAATAACTGAGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtg                   <  2:1214732/97‑1 (MQ=255)
                                                                           tAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtgtg                 >  2:3158249/1‑100 (MQ=255)
                                                                           tAAGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtgtg                 <  1:2061800/100‑1 (MQ=255)
                                                                           tAACGAACAAAGATAAGCGTCCCTGGAACGGTAACGAATTATAGAAAGGCGCGAACAACTTCGCAATGTATTCTTATTTCATTTTTTGAATAAGCAtgt                  >  2:342171/1‑99 (MQ=255)
                                                                            aaGGATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTgg                >  2:1074201/1‑100 (MQ=255)
                                                                              ggATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCg              <  2:1221921/100‑1 (MQ=255)
                                                                               gATCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGa             >  1:1935310/1‑100 (MQ=255)
                                                                                aTCAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGaa            >  2:196177/1‑100 (MQ=255)
                                                                                 tCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGaaa           <  1:1557964/100‑1 (MQ=255)
                                                                                  cAAAGATTAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGaaaa          >  1:3776501/1‑100 (MQ=255)
                                                                                      gATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGa      >  2:2353216/1‑100 (MQ=255)
                                                                                       aTTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAt     >  2:1814874/1‑100 (MQ=255)
                                                                                        ttAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtt    <  1:1087013/100‑1 (MQ=255)
                                                                                        ttAGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtt    <  1:1087000/100‑1 (MQ=255)
                                                                                          aGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtttt  <  2:1886824/100‑1 (MQ=255)
                                                                                          aGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGAtttt  >  1:716464/1‑100 (MQ=255)
                                                                                          aGCGCCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATCTGGCGAAAACAGAtttt  >  2:1133781/1‑100 (MQ=255)
                                                                                              |                                                                                               
ACTGTCTTCAAAAGCTTCATTTATCAAGAGTCCGTACAACAAAAAAAGAGACCATCGCGGTCCCGGAAACTTTCTTAAGGATCAAAGATTAGCGTCCCTGGAAAGGTAACGAATTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTT  >  NC_000913/1806064‑1806253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A