Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,682,158 T→C 76.1% K196K (AAA→AAG)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,682,1580TC76.1% 261.4 / 70.8 133K196K (AAA→AAG) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base T (11/21);  new base C (45/56);  total (56/77)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

GCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGG  >  NC_000913/2682062‑2682257
                                                                                                |                                                                                                   
gCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTc                                                                                                  <  2:2209055/100‑1 (MQ=255)
  cGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCAc                                                                                                >  2:815917/1‑100 (MQ=255)
  cGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCAc                                                                                                <  2:1294789/100‑1 (MQ=255)
  cGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCAc                                                                                                <  1:487293/100‑1 (MQ=255)
  cGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTGGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCAc                                                                                                <  2:3995451/100‑1 (MQ=255)
     tAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGc                                                                                             >  1:3990255/1‑100 (MQ=255)
        cGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCgg                                                                                          >  1:1284392/1‑100 (MQ=255)
        cGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCgg                                                                                          <  1:3532790/100‑1 (MQ=255)
        cGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCgg                                                                                          >  1:1582282/1‑100 (MQ=255)
         gATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCggg                                                                                         >  1:2994333/1‑100 (MQ=255)
           tGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGtt                                                                                       >  1:3232095/1‑100 (MQ=255)
             gggAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTgg                                                                                     <  2:1617874/100‑1 (MQ=255)
              ggAAAGCGGAGACGGCCCGTTTGTCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGc                                                                                    <  2:541364/100‑1 (MQ=255)
              ggAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATGCGGCGGGAAGACGCTCTGATGCCCTGCCCCCCCCTTCACTGCCTGGTTGGc                                                                                    <  2:1692879/100‑1 (MQ=255)
              ggAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGc                                                                                    <  1:3385542/100‑1 (MQ=255)
              ggAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCACCTTCACTGCCGGGTTGGc                                                                                    >  2:2700879/1‑100 (MQ=255)
                aaaGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCTCTTCACTGCCGGGTTGGccc                                                                                  <  2:2544011/100‑1 (MQ=255)
                aaaGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGccc                                                                                  <  2:580153/100‑1 (MQ=255)
                aaaGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGccc                                                                                  <  1:3099193/100‑1 (MQ=255)
                 aaGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGccc                                                                                  >  2:3590918/1‑99 (MQ=255)
                   gCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCccacc                                                                               <  1:3812168/100‑1 (MQ=255)
                   gCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCccacc                                                                               <  1:1986361/100‑1 (MQ=255)
                   gCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCccacc                                                                               <  2:1776155/100‑1 (MQ=255)
                   gCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCccacc                                                                               <  2:652406/100‑1 (MQ=255)
                     ggAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCcaccac                                                                             >  1:1824628/1‑100 (MQ=255)
                       agaCGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACaa                                                                           <  1:4145889/100‑1 (MQ=255)
                        gaCGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAg                                                                          <  1:2898605/100‑1 (MQ=255)
                         aCGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGa                                                                         <  1:3656507/100‑1 (MQ=255)
                          cGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGCTGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGaa                                                                        <  1:60713/100‑1 (MQ=255)
                          cGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGaa                                                                        >  1:2410177/1‑100 (MQ=255)
                          cGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGaa                                                                        >  1:4026758/1‑100 (MQ=255)
                           ggCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACaa                                                                           >  2:2858417/1‑96 (MQ=255)
                            gCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAAt                                                                      >  1:3816046/1‑100 (MQ=255)
                             cccGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATg                                                                     >  1:685772/1‑100 (MQ=255)
                              ccGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGa                                                                    <  2:2466087/100‑1 (MQ=255)
                                tcttgtggTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg                                                                  <  1:148577/93‑1 (MQ=255)
                                gTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg                                                                  <  1:3059095/100‑1 (MQ=255)
                                     ccGTGATCAAAGACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTTACTGCCGGGTTGGACCACCACAAGAAAt                                                                      >  1:3262769/1‑91 (MQ=255)
                                     ccGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACgcggcg                                                             >  1:23486/1‑100 (MQ=255)
                                      cGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgt                                                            >  2:2203979/1‑100 (MQ=255)
                                       gTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAACTGACgcggc                                                              >  2:1468857/1‑97 (MQ=255)
                                       gTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAACGACgcggcg                                                             >  2:3688488/1‑98 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGCCGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          <  2:2404701/100‑1 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          <  2:3776655/100‑1 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          <  2:2087112/100‑1 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGTCCACCACAAGAAATGACGCggcgtgg                                                          <  2:3509371/100‑1 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACgcgccg                                                             >  2:1482189/1‑97 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          <  2:3907297/100‑1 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          <  2:3075916/100‑1 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          <  1:976268/100‑1 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          >  1:2523058/1‑100 (MQ=255)
                                        tGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTGCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtgg                                                          <  2:1115290/100‑1 (MQ=255)
                                         gATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggc                                                         >  1:1845294/1‑100 (MQ=255)
                                         gATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggc                                                         <  1:428519/100‑1 (MQ=255)
                                          aTCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggcg                                                        >  1:3418034/1‑100 (MQ=255)
                                           tCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtggcgt                                                       >  1:888111/1‑100 (MQ=255)
                                            cAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCCCCACAAGAAATGACGCGGCGTGGCGtt                                                      >  2:171662/1‑100 (MQ=255)
                                            cAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt                                                      <  2:1482970/100‑1 (MQ=255)
                                             aaaCACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTc                                                     >  1:2032022/1‑100 (MQ=255)
                                              aaCACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTcc                                                    <  1:1398376/100‑1 (MQ=255)
                                                cacCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCg                                                  <  1:1186814/100‑1 (MQ=255)
                                                cacCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCg                                                  <  2:3192796/100‑1 (MQ=255)
                                                   cgccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTta                                               <  2:712794/100‑1 (MQ=255)
                                                    gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTtat                                              <  1:2587690/100‑1 (MQ=255)
                                                    gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTta                                               <  2:2029984/99‑1 (MQ=255)
                                                    gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTCCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTtat                                              >  1:2331975/1‑100 (MQ=255)
                                                      cgTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑CCCCCCTTTCGCTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGa                                           <  2:236271/100‑1 (MQ=255)
                                                       gTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGa                                           <  2:504543/100‑1 (MQ=255)
                                                         tACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGAcg                                         <  1:2128421/100‑1 (MQ=255)
                                                           cATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGAcgcg                                       <  1:380135/100‑1 (MQ=255)
                                                             tCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGc                                     >  2:1179116/1‑100 (MQ=255)
                                                              ccGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACACGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCgg                                      >  2:1544936/1‑98 (MQ=255)
                                                               cggcggGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTg                                   <  1:3131479/100‑1 (MQ=255)
                                                               cggcggGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTg                                   >  1:1776438/1‑100 (MQ=255)
                                                                ggcggGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTgg                                  <  2:3314203/100‑1 (MQ=255)
                                                                 gcggGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGc                                 <  2:3959356/100‑1 (MQ=255)
                                                                 gcggGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGc                                 <  1:531878/100‑1 (MQ=255)
                                                                  cggGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTTGCGTTCCCGTTATAGACGCGGCTGGCg                                <  2:12919/100‑1 (MQ=255)
                                                                  cggGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCg                                >  1:3250468/1‑100 (MQ=255)
                                                                   gggAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGa                               <  1:2593094/100‑1 (MQ=255)
                                                                    ggAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGAt                              >  1:3934190/1‑100 (MQ=255)
                                                                    ggAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCAAAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGAt                              >  1:3057307/1‑100 (MQ=255)
                                                                     gAAGACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGAtt                             <  1:3424854/100‑1 (MQ=255)
                                                                        gACGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTcc                          <  1:2324528/100‑1 (MQ=255)
                                                                         aCGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCa                         <  1:2151461/100‑1 (MQ=255)
                                                                          cGCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTcc                          >  2:1312735/1‑98 (MQ=255)
                                                                           gCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAgc                       <  2:1929549/100‑1 (MQ=255)
                                                                           gCTCTGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAgc                       <  1:1532349/100‑1 (MQ=255)
                                                                            ctctGATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAgcg                      <  2:1158476/100‑1 (MQ=255)
                                                                               tGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCAAAAGAAAAGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGcgccg                   >  1:295655/1‑100 (MQ=255)
                                                                                gATGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGcgccgc                  >  1:3128110/1‑100 (MQ=255)
                                                                                  tGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGccc                >  2:2610991/1‑100 (MQ=255)
                                                                                  tGCCCTTCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGccc                <  1:2642843/100‑1 (MQ=255)
                                                                                       ttCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCa           <  2:3253779/100‑1 (MQ=255)
                                                                                       ttCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCa           <  2:2830580/100‑1 (MQ=255)
                                                                                        tCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAg          >  1:2064137/1‑100 (MQ=255)
                                                                                        tCCCCCCCCTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAg          >  1:607542/1‑100 (MQ=255)
                                                                                         cccccccTTTCACTGCCGGGTTGGCCCACCACAAGAACTGACGCGGCGTGGCGTTCCCGTTATAGACGAGCCTGGCGATTTCCAGCGCCGCCCgg              >  2:3662931/1‑95 (MQ=255)
                                                                                             ccccTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAggg        >  2:496028/1‑97 (MQ=255)
                                                                                             ccccTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc     >  1:530678/1‑100 (MQ=255)
                                                                                                |                                                                                                   
GCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGG  >  NC_000913/2682062‑2682257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A