Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F133 I0 R1 200 171.4 8351354 94.3% 7875326 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,627,706 T→G 18.8% F131C (TTT→TGT)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,627,7060TG18.8% 392.4 / 91.3 173F131C (TTT→TGT) nadRtrifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (79/60);  new base G (19/13);  total (100/73)
Fisher's exact test for biased strand distribution p-value = 8.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 185 total aligned reads displayed.

TGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGT  >  NC_000913/4627607‑4627804
                                                                                                   |                                                                                                  
tGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACttt                                                                                                    >  1:3708610/1‑100 (MQ=255)
tGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACttt                                                                                                    >  1:365378/1‑100 (MQ=255)
 gggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACtttt                                                                                                   <  1:2058579/100‑1 (MQ=255)
  ggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTa                                                                                                  >  1:1874949/1‑100 (MQ=255)
  ggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTa                                                                                                  <  1:182612/100‑1 (MQ=255)
  ggTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTa                                                                                                  <  1:483009/100‑1 (MQ=255)
   gTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTaa                                                                                                 <  2:2989723/100‑1 (MQ=255)
   gTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTaa                                                                                                 >  1:3227141/1‑100 (MQ=255)
   gTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTaa                                                                                                 >  2:3191700/1‑100 (MQ=255)
   gTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTaa                                                                                                 >  1:3836740/1‑100 (MQ=255)
    ttttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTaaa                                                                                                <  2:2321715/100‑1 (MQ=255)
     tttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAat                                                                                               >  1:1588976/1‑100 (MQ=255)
     tttGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAat                                                                                               <  2:1822883/100‑1 (MQ=255)
       tGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGCCTGCGTTGGTTATTGCAAACTTTTAAatat                                                                                             >  1:1988573/1‑100 (MQ=255)
        gACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGTTTATTGCAAACTTGTAAATATc                                                                                            <  2:2418326/100‑1 (MQ=255)
         aCGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCa                                                                                           >  1:3328913/1‑100 (MQ=255)
          cGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAg                                                                                          <  1:3927955/100‑1 (MQ=255)
           gATACCCGTGACCACGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGa                                                                                         >  2:2583570/1‑100 (MQ=255)
            aTACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGaa                                                                                        >  2:818412/1‑100 (MQ=255)
             tACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGaaa                                                                                       <  1:2603436/100‑1 (MQ=255)
               cccGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGaaaaa                                                                                     >  1:2172258/1‑100 (MQ=255)
               cccGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGaaaaa                                                                                     >  1:1384882/1‑100 (MQ=255)
                  gTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAatat                                                                                  <  2:3328888/100‑1 (MQ=255)
                  gTGACCGCGCGTTGTGCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAatat                                                                                  <  2:561966/100‑1 (MQ=255)
                     aCCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCg                                                                               <  2:2392697/100‑1 (MQ=255)
                      ccGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGc                                                                              >  1:2513748/1‑100 (MQ=255)
                       cgcgcgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCa                                                                             <  2:2609880/100‑1 (MQ=255)
                           cgTTGTTCGAAGACAGTGCCATGTCGTAGCATCCGATCGTGCCGGATCGTCTGCGTTTGTTATTGCTATCTTTTAAATTTCAGAAAAATATTCGCATTCa                                                                         <  2:446157/100‑1 (MQ=255)
                           cgTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCa                                                                         >  1:1116425/1‑100 (MQ=255)
                               gttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCt                                                                     >  2:2367069/1‑100 (MQ=255)
                               gttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCt                                                                     >  2:1860591/1‑100 (MQ=255)
                               gttCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCt                                                                     <  1:931295/100‑1 (MQ=255)
                                  cGAGGACAGTGCCATGTCGGAGCAGGCGACCGTGCCGGATCGTCTGAGATGGTTATTGCAAACTTATAAATATCAGAAAAATATTCGCATTCATGCGTTc                                                                  >  2:737332/1‑100 (MQ=255)
                                  cGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTc                                                                  >  2:290175/1‑100 (MQ=255)
                                  cGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTc                                                                  <  2:365375/100‑1 (MQ=255)
                                  cGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTc                                                                  >  1:101202/1‑100 (MQ=255)
                                  cGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATACATGCTTTc                                                                  >  2:2921483/1‑100 (MQ=255)
                                   gAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCa                                                                 <  2:96904/100‑1 (MQ=255)
                                   gAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCa                                                                 >  1:1490711/1‑100 (MQ=255)
                                    aaGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCaa                                                                <  2:1374489/100‑1 (MQ=255)
                                    aaGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCaa                                                                >  2:2297262/1‑100 (MQ=255)
                                     aGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAAc                                                               <  1:1561535/100‑1 (MQ=255)
                                      gACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACg                                                              <  1:1656971/100‑1 (MQ=255)
                                      gACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACg                                                              >  2:1372745/1‑100 (MQ=255)
                                      gACAGAGCCATGTCGCAGCAGCCGACCGTGACGGATCGTCTGCGTTGGTTATTTTAAACTTTAAAAAAAAAAAAAAATATTCGCATTCATGCTTTCAAc                                                               <  2:4128394/99‑1 (MQ=255)
                                        cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGaa                                                            >  1:980003/1‑100 (MQ=255)
                                        cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGaa                                                            >  2:3150791/1‑100 (MQ=255)
                                        cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGaa                                                            >  2:2018513/1‑100 (MQ=255)
                                        cAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGa                                                             >  2:66454/1‑99 (MQ=255)
                                             ccATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAggg                                                       >  1:2844666/1‑100 (MQ=255)
                                             ccATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAggg                                                       >  1:117154/1‑100 (MQ=255)
                                               aTGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAgggaa                                                     <  1:2823992/100‑3 (MQ=255)
                                               aTGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCa                                                     >  1:2229200/1‑100 (MQ=255)
                                                  tCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATgg                                                  <  1:762574/100‑1 (MQ=255)
                                                    gcagcaGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAg                                                >  1:4065360/1‑100 (MQ=255)
                                                    gcagcaGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAg                                                >  1:725280/1‑100 (MQ=255)
                                                     cagcagCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGc                                               <  1:3295688/100‑1 (MQ=255)
                                                     cagcagCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGc                                               >  2:892127/1‑100 (MQ=255)
                                                       gcagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCg                                             >  2:608752/1‑100 (MQ=255)
                                                        cagcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGt                                            >  2:3462033/1‑100 (MQ=255)
                                                         agcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTa                                           <  1:2363397/100‑1 (MQ=255)
                                                          gcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTAt                                          >  1:2897537/1‑100 (MQ=255)
                                                          gcCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGACATGGAGCCGt                                            >  2:3442725/1‑98 (MQ=255)
                                                            agACCGTGCCGGATCGTCTGCGTTGTTTAATGCAACCGTGTAAATATAAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTa                                           <  2:4114629/96‑1 (MQ=255)
                                                                 gTGCCGGATCGACTGCGTTGGTTGTTGCAAACTTTAAAAAAACAAAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATc                                         <  2:349857/94‑1 (MQ=255)
                                                                   gCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGc                                 >  2:1535328/1‑100 (MQ=255)
                                                                     cGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTg                               >  1:1298756/1‑100 (MQ=255)
                                                                      ggATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTgg                              >  1:3987799/1‑100 (MQ=255)
                                                                       gATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTggg                             >  2:2245067/1‑100 (MQ=255)
                                                                         tCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAt                           <  2:1588967/100‑1 (MQ=255)
                                                                           gTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgt                         >  1:457872/1‑100 (MQ=255)
                                                                           gTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgt                         >  1:431030/1‑100 (MQ=255)
                                                                             cTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtgtg                        >  2:2404385/1‑99 (MQ=255)
                                                                               gCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTgg                     >  1:2122217/1‑100 (MQ=255)
                                                                               gCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTgg                     <  1:1223920/100‑1 (MQ=255)
                                                                               gCGTTGGTTATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTgg                     <  2:486155/100‑1 (MQ=255)
                                                                               gCGTTGGTTATTGCAAACTTGTAAATATCAAAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTCGGATGTGTgg                     >  2:2739404/1‑100 (MQ=255)
                                                                                 gTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                   >  1:2283410/1‑100 (MQ=255)
                                                                                 gTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                   <  1:1050131/100‑1 (MQ=255)
                                                                                 gTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                   <  1:2018523/100‑1 (MQ=255)
                                                                                 gTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAg                   <  1:3480003/100‑1 (MQ=255)
                                                                                   tagTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGc                  <  1:3781840/97‑1 (MQ=255)
                                                                                      ttATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACg              <  1:397485/100‑1 (MQ=255)
                                                                                      ttATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACg              <  1:3684556/100‑1 (MQ=255)
                                                                                       tATTGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACgg             <  2:3674270/100‑1 (MQ=255)
                                                                                          tGCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCAt          <  1:881175/100‑1 (MQ=255)
                                                                                           gCAAACTTTTAAATATCAGAAAAATATTCGCCTTCATGCTTTCAACGAAAACGGCATCGAGCCATATCCGCACCGCTGGGATGTGAGGAGAAACGGCATc         >  2:2920266/1‑100 (MQ=255)
                                                                                           gCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATc         >  2:1693726/1‑100 (MQ=255)
                                                                                           gCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATc         >  2:3606907/1‑100 (MQ=255)
                                                                                           gCAAACTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGACCCGTATCCGCACGCCTGGGATGTGTGGAACAACAGCATc         >  2:135788/1‑100 (MQ=255)
                                                                                            cAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATc         >  2:3084546/1‑99 (MQ=255)
                                                                                             aaaCTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCaa       <  1:2751795/100‑1 (MQ=255)
                                                                                             aaaCTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCaa       >  2:38677/1‑100 (MQ=255)
                                                                                                cTTGTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGCTGTGTGGAGCAACGGCATCaaaaa    >  1:1282611/1‑100 (MQ=255)
                                                                                                 ttttAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAg   <  1:852570/100‑1 (MQ=255)
                                                                                                 ttttAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAg   <  2:3805291/100‑1 (MQ=255)
                                                                                                 ttttAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAg   >  1:1953678/1‑100 (MQ=255)
                                                                                                  tttAAATATCAGAAAAATATTCGCATTCATGCTTTCAATGAAGAGGTCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGt  <  2:2002482/100‑1 (MQ=255)
                                                                                                  tttAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAg   <  2:3804614/99‑1 (MQ=255)
                                                                                                  tttAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGt  >  1:30730/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGT  >  NC_000913/4627607‑4627804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A