Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
254,727 |
A→C |
G330G (GGT→GGG) |
pepD ← |
aminoacyl‑histidine dipeptidase (peptidase D) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 254,727 | 0 | A | C | 96.4%
| 191.4
/ ‑0.7
| 57 | G330G (GGT→GGG) | pepD | aminoacyl‑histidine dipeptidase (peptidase D) |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (31/23); total (33/24) |
| Fisher's exact test for biased strand distribution p-value = 5.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01 |
CAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAG > NC_000913/254632‑254820
|
cAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGt > 2:112127/1‑100 (MQ=255)
tGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCg > 1:650677/1‑100 (MQ=255)
aTTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCgggg > 1:384042/1‑100 (MQ=255)
tttCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACGCCTTTGGCTACATCGGAGTTACGAATCACTCCGTTCgggg < 1:444860/99‑1 (MQ=255)
ttCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTg > 2:63707/1‑100 (MQ=255)
ttCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTg > 2:521549/1‑100 (MQ=255)
tCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTgg < 2:818824/100‑1 (MQ=255)
tCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTgg > 2:250728/1‑100 (MQ=255)
tACGTTATTGGCAGTCATGGTCACCACACCGGCGTTCATGGACGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCg < 2:169723/100‑1 (MQ=255)
cGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTgattcg < 1:957448/100‑6 (MQ=255)
cGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGtt > 1:350140/1‑100 (MQ=255)
gTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTc < 1:933258/100‑1 (MQ=255)
gTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTc > 2:146725/1‑100 (MQ=255)
gTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTc > 2:146760/1‑100 (MQ=255)
aTTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTcagc < 1:1073912/100‑1 (MQ=255)
cagtcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACg > 2:1184361/1‑100 (MQ=255)
cagtcaTGGTCACCACACCGACGTTCAGGGAGGTTTCAACAACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACg > 2:242571/1‑100 (MQ=255)
gTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAgg < 2:434549/100‑1 (MQ=255)
gTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAgg < 1:508592/100‑1 (MQ=255)
tCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGt > 1:914547/1‑100 (MQ=255)
caccacACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTa > 2:1273782/1‑100 (MQ=255)
accacACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTAt > 1:532531/1‑100 (MQ=255)
ccacACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATc > 2:490027/1‑100 (MQ=255)
cacCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATcgcg < 1:37141/100‑1 (MQ=255)
ccGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCgag < 2:134856/100‑1 (MQ=255)
gTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTc < 2:1137139/100‑1 (MQ=255)
tCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGc < 2:415224/100‑1 (MQ=255)
tCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGc < 2:650673/100‑1 (MQ=255)
tCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGc > 1:274845/1‑100 (MQ=255)
aGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCaa > 1:718011/1‑100 (MQ=255)
gggAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAAt > 2:664940/1‑100 (MQ=255)
gggAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAAt > 1:842997/1‑100 (MQ=255)
gggAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAAt > 1:1029453/1‑100 (MQ=255)
gggAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAAt > 1:51478/1‑100 (MQ=255)
ggTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAgg < 1:1188211/100‑1 (MQ=255)
gTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAggg > 2:237608/1‑100 (MQ=255)
cacaCCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGAttt > 1:55026/1‑99 (MQ=255)
acCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCg < 2:51477/100‑1 (MQ=255)
acCTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCg < 1:356238/100‑1 (MQ=255)
ccTTTGGCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGt > 1:548888/1‑100 (MQ=255)
ggCTACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCt > 1:232660/1‑100 (MQ=255)
tACATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACa < 2:51395/100‑1 (MQ=255)
aCATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGATATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACag < 1:920150/100‑1 (MQ=255)
aCATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACag > 1:1213612/1‑100 (MQ=255)
cATCGGAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACaga < 1:431365/100‑1 (MQ=255)
gAGTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCa > 1:723462/1‑100 (MQ=255)
gTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGc < 1:433847/100‑1 (MQ=255)
gTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGc < 1:521553/100‑1 (MQ=255)
gTTACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGc < 1:193491/100‑1 (MQ=255)
ttACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGca < 1:1041032/100‑1 (MQ=255)
tACGAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGcaa > 1:966147/1‑100 (MQ=255)
ctaaTCACCCCGTTCGGGGTGGCGTTCAGAAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGcaaca < 2:512653/98‑1 (MQ=255)
ggaTCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGcaacaa > 1:880008/3‑100 (MQ=255)
gAATCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGcaacaa > 1:1015956/1‑100 (MQ=255)
aaTCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAg > 1:26066/1‑100 (MQ=255)
aaTCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAg > 1:562138/1‑100 (MQ=255)
aaTCACCCCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAg > 1:156319/1‑100 (MQ=255)
|
CAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAG > NC_000913/254632‑254820
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
