Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,870,998 |
(T)6→5 |
coding (614/1476 nt) |
yeaI → |
putative membrane‑anchored diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,870,993 | 0 | T | . | 96.5%
| 248.4
/ 2.0
| 58 | coding (609/1476 nt) | yeaI | putative membrane‑anchored diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base . (29/27); total (30/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.58e-01 |
TTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGC > NC_000913/1870896‑1871088
|
tttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttt > 2:718667/1‑100 (MQ=255)
ttGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttt < 2:459687/100‑1 (MQ=255)
tGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttttt > 1:442479/1‑100 (MQ=255)
tGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttttt > 1:216217/1‑100 (MQ=255)
gattAACCTCGCTGGCGCTCTTTTTTTATGGAAAAGACAACATCCTTTACAACAATAAG‑AAAATCACGGGAATCCTGTTGCTGGCGCTGATCCCttttt < 2:617864/97‑1 (MQ=255)
tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACGACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttttag > 2:699016/1‑98 (MQ=255)
tttAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCtttttag > 1:68236/1‑98 (MQ=255)
ccTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGttttt > 1:451550/1‑100 (MQ=255)
cGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTccc < 1:1017583/100‑1 (MQ=255)
gCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTcctc > 2:793428/1‑98 (MQ=255)
cTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTccccn < 1:1098271/100‑2 (MQ=255)
gcTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTgg < 1:721716/100‑1 (MQ=255)
gcTCTTTTGTTATGGAAAAGACAACATCCTTGAAAAAAAAAAA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTgg < 2:364531/100‑1 (MQ=255)
ctctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGc < 2:969841/100‑1 (MQ=255)
ctctTTTGTTATGGAAAAGACAACATCCTTAACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGc < 1:46262/100‑1 (MQ=255)
ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGca > 1:981630/1‑100 (MQ=255)
ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGccc > 2:585989/1‑98 (MQ=255)
ctTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGcac > 1:830269/1‑100 (MQ=255)
ctTTTGTTATGGAAAAGACAACATCCTTGACAAAAAAAAA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGc < 2:1064391/98‑1 (MQ=255)
ttGTTATGGAAAAGACAACATCCTTGACAACAATATA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGcacaca < 2:289225/100‑1 (MQ=255)
tGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACaa > 2:888156/1‑100 (MQ=255)
gTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAAt > 1:322718/1‑100 (MQ=255)
ttATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATc < 2:559943/100‑1 (MQ=255)
ttATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATc < 2:303137/100‑1 (MQ=255)
aTGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCtn < 2:528238/100‑2 (MQ=255)
aTGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTg > 2:275913/1‑100 (MQ=255)
ggAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg > 1:404105/1‑100 (MQ=255)
gAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACACTCTGAGc > 2:1110106/1‑100 (MQ=255)
gAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc > 1:29694/1‑100 (MQ=255)
aaaGACAACCTCCTTGACAACAATAAGAAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCa < 1:572383/100‑1 (MQ=255)
aGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGtt < 1:720131/100‑1 (MQ=255)
gACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGtt > 1:351377/1‑99 (MQ=255)
gACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTta < 1:1050681/100‑1 (MQ=255)
gACAACAACCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTta < 2:1134168/100‑1 (MQ=255)
acaacaTCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTtat > 1:713440/1‑100 (MQ=255)
tCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCt > 1:15851/1‑100 (MQ=255)
tCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAACCTGAGCAGTTATAACGCt > 1:674233/1‑100 (MQ=255)
ttGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGAc < 2:163470/100‑1 (MQ=255)
acaacaATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAt > 2:415566/1‑100 (MQ=255)
aaaacaaAAAA‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTa < 2:101989/97‑1 (MQ=255)
aacaaTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTc < 1:608864/100‑1 (MQ=255)
acaaTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCt > 1:1104030/1‑100 (MQ=255)
aTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTg < 2:1064957/100‑1 (MQ=255)
aTAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTg < 2:1171266/100‑1 (MQ=255)
aaG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTa < 1:1030626/100‑1 (MQ=255)
aaG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTa > 2:1030617/1‑100 (MQ=255)
aG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTAt > 2:909716/1‑100 (MQ=255)
aG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTAt > 1:1086977/1‑100 (MQ=255)
g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg > 1:1063830/1‑100 (MQ=255)
g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg < 1:925262/100‑1 (MQ=255)
g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg > 2:587740/1‑100 (MQ=255)
g‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATg < 1:1133054/100‑1 (MQ=255)
acaaaaCGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtc > 1:882618/3‑100 (MQ=255)
aaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtc > 2:1187199/1‑100 (MQ=255)
aaaaaaCGGGAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATgtc > 2:88945/1‑100 (MQ=255)
gAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTAc > 2:172352/1‑100 (MQ=255)
gAATCCTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTAc > 2:913250/1‑100 (MQ=255)
cTGTTGCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTcc < 2:1117019/100‑1 (MQ=255)
tGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTcc < 2:981622/100‑1 (MQ=255)
ttGCTGGCGTTGATCCC‑TTTATCGTATCTGCTCTTCTCGCACACACTCTCAGACGTACTTACGCTGACTATTCTTTCTATTTCGACGATCACTGTCCg < 2:760480/98‑1 (MQ=255)
gCTGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACa > 1:562084/1‑100 (MQ=255)
tGGCGCTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAAc > 2:477466/1‑100 (MQ=255)
cgcTGATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCAt > 1:368232/1‑100 (MQ=255)
gATCCC‑TTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTg < 2:351374/100‑1 (MQ=255)
aTCCC‑TTTTTAGTTTTTCCTCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGc < 2:442475/100‑1 (MQ=255)
|
TTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAG‑AAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGC > NC_000913/1870896‑1871088
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
