Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,239,779 |
C→T |
intergenic (+234/‑165) |
alx → / → sstT |
putative membrane‑bound redox modulator/sodium:serine/threonine symporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,239,779 | 0 | C | T | 90.0%
| 161.6
/ 7.1
| 60 | intergenic (+234/‑165) | alx/sstT | putative membrane‑bound redox modulator/sodium:serine/threonine symporter |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (29/25); total (33/27) |
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 |
CCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGT > NC_000913/3239682‑3239875
|
cccAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCa < 2:1184713/100‑1 (MQ=255)
cAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCAtt > 1:448303/1‑100 (MQ=255)
aaCGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTaa > 2:566249/1‑100 (MQ=255)
aCGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAg > 2:1170676/1‑100 (MQ=255)
ggggCGAGGGGAGTGACCGAGTCCTTTTTTGATGTTGTCATGGGTCTGGAACCCGCACGTTGGCTTTGTTTTTATGTTAAAGCAATGTAATCAATAAGt > 2:118582/1‑99 (MQ=255)
gggACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAata < 2:747550/100‑1 (MQ=255)
gggACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAata < 1:1247455/100‑1 (MQ=255)
gACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATg < 2:420655/100‑1 (MQ=255)
gACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTCATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATg < 2:694641/100‑1 (MQ=255)
aCTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGa < 1:554873/100‑1 (MQ=255)
cTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGAc > 1:132112/1‑100 (MQ=255)
gACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACct > 2:729230/1‑100 (MQ=255)
gACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACct > 1:204956/1‑100 (MQ=255)
gACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACct > 2:1057349/1‑100 (MQ=255)
gACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTGGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACct > 1:211400/1‑100 (MQ=255)
aCCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACctc > 2:18859/1‑100 (MQ=255)
ccGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACctct > 1:179806/1‑100 (MQ=255)
cGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACctctc > 1:604387/1‑100 (MQ=255)
gAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACctctct > 2:269234/1‑100 (MQ=255)
cTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTaaa < 1:219439/100‑1 (MQ=255)
tttGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATcc < 2:237928/100‑1 (MQ=255)
tGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAg > 1:1216599/1‑100 (MQ=255)
gATGTTGTCATCAGTCCGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGc < 2:763691/100‑1 (MQ=255)
aTGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCa > 2:722114/1‑100 (MQ=255)
catcaGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCg < 2:1254151/100‑1 (MQ=255)
catcaGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCg > 1:961233/1‑100 (MQ=255)
caGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCtt > 2:211655/1‑100 (MQ=255)
caGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCtt < 1:1170687/100‑1 (MQ=255)
aGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCAttttttttttt > 2:938140/1‑94 (MQ=255)
gTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTcc < 2:424397/100‑1 (MQ=255)
gTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTcc < 2:16440/100‑1 (MQ=255)
tCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTccc < 1:838192/100‑1 (MQ=255)
tCTGGAAGCCGCACGTTGGCTTTATTTGTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTccc < 1:194643/100‑1 (MQ=255)
cTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCg > 1:915885/1‑100 (MQ=255)
cTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGAATTTTTCGCt > 2:6814/1‑95 (MQ=255)
tGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCg < 2:495685/99‑1 (MQ=255)
tGGAAGCCGCACGTTGGCTTTATCTTTATGTCAAAGAAATGTAATCATTTAGTTTCAAAATATGACCTCACGTTAAAAAACAGCAttttttccacccc > 2:260541/1‑90 (MQ=255)
gAAGCCGCACGTTGGCTTTATTTTTATGTCAACGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAg < 1:242375/100‑1 (MQ=255)
aaGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGc < 1:745052/100‑1 (MQ=255)
aCGTTGGCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACt > 2:428599/1‑100 (MQ=255)
ggCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCACAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCt < 2:913136/100‑1 (MQ=255)
ggCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCt < 1:1002297/100‑1 (MQ=255)
gCTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACTTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCtt > 1:582738/1‑100 (MQ=255)
cTTTATTTTTATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTta < 1:847453/100‑1 (MQ=255)
cTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTta > 1:826545/1‑100 (MQ=255)
tATCTTTATGTCAAAGAAATGTAACCATTAAGTTTCACAAGATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATAc < 2:1173327/100‑1 (MQ=255)
tttttATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTc > 2:541414/1‑100 (MQ=255)
tttttATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTAGTTTCGCGAAGCTGTAACTTTCCTAATACTc > 2:826495/1‑100 (MQ=255)
tttATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGa > 2:874148/1‑100 (MQ=255)
ttATGTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGAc > 1:553218/1‑100 (MQ=255)
gTCAAAGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTg < 1:425738/100‑1 (MQ=255)
gTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTg > 1:455701/1‑100 (MQ=255)
aaaGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCaa > 1:692089/1‑100 (MQ=255)
aaGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCaaa < 1:945668/100‑1 (MQ=255)
aaGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCCGCATTTTTCGCTTCCCGACGCTGTAACTTTCCTTATACTCGACCTTg > 2:254421/1‑96 (MQ=255)
aGAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAac < 1:18859/100‑1 (MQ=255)
gAAATGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAaca > 1:389670/1‑100 (MQ=255)
aaaTGTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAacac < 2:842794/100‑1 (MQ=255)
gTAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTg < 2:448299/100‑1 (MQ=255)
tAATCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGt > 1:477742/1‑100 (MQ=255)
|
CCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCATTTTTCGCTTCCCGAAGCTGTAACTTTCCTTATACTCGACCTTGCAAACACTTTGT > NC_000913/3239682‑3239875
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
