Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,608,471 |
T→A |
L674Q (CTG→CAG) |
zntA → |
zinc, cobalt and lead efflux system |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,608,471 | 0 | T | A | 97.7%
| 150.8
/ ‑5.0
| 44 | L674Q (CTG→CAG) | zntA | zinc, cobalt and lead efflux system |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (26/17); total (26/18) |
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG > NC_000913/3608380‑3608567
|
gCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCAcgcg > 1:531225/1‑100 (MQ=255)
gCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCAcgcg > 1:512942/1‑100 (MQ=255)
aaTGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGcc > 1:1283657/1‑100 (MQ=255)
ggTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTc > 2:1081429/1‑100 (MQ=255)
gCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGc > 1:19007/1‑100 (MQ=255)
gCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGAGCCAATGATTGAACAGGCACGCGCCACTCACGc > 2:904676/1‑100 (MQ=255)
cGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGcc < 2:1251329/100‑1 (MQ=255)
ggCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCa > 2:966503/1‑100 (MQ=255)
cacaGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAat < 1:216779/100‑1 (MQ=255)
aCGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCg < 1:1255742/100‑1 (MQ=255)
gcgcTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGa > 1:735520/1‑100 (MQ=255)
cTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACa < 1:158789/100‑1 (MQ=255)
tGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACAt < 1:633147/100‑1 (MQ=255)
ggAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATc > 2:1052066/1‑100 (MQ=255)
cgccgACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTAtt > 1:1302140/1‑100 (MQ=255)
gcagcaTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCGCGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTgg < 2:725588/100‑1 (MQ=255)
aTTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTg > 2:1048304/1‑100 (MQ=255)
ttAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGa < 1:638304/100‑1 (MQ=255)
tAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTgga < 1:881586/100‑3 (MQ=255)
tAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGaa < 2:662289/100‑1 (MQ=255)
aCCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAg < 2:512938/100‑1 (MQ=255)
cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg > 2:37844/1‑100 (MQ=255)
cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg > 2:318307/1‑100 (MQ=255)
cccATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg > 1:1035132/1‑100 (MQ=255)
cccATAACAACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGACAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAgg < 2:1110382/100‑1 (MQ=255)
cATAACCGCCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACGCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGa < 2:218642/100‑1 (MQ=255)
cATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGa < 1:1029568/100‑1 (MQ=255)
aaCCACCTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCt > 1:286822/1‑100 (MQ=255)
accaccTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCtt > 1:601564/1‑100 (MQ=255)
caccTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATGTCCGCCAGAACTTCACTATTGCGCTGGGGCTAAAAGGGATCTTcc < 2:536765/100‑1 (MQ=255)
accTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATGGCGCTGGGGCGGAGAGGGACCTTcc > 1:191935/1‑99 (MQ=255)
cTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg < 2:1098977/100‑1 (MQ=255)
cTGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCg > 1:366193/1‑100 (MQ=255)
tGCGCGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGt > 2:840330/1‑100 (MQ=255)
gcgcGGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTc > 1:259311/1‑100 (MQ=255)
gcgcGACCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTc > 1:1304024/1‑100 (MQ=255)
cgGCCTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTcacc > 2:693482/1‑100 (MQ=255)
cTGGTGCAAATGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGc > 1:552086/1‑100 (MQ=255)
aaTGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCCCTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAggg < 2:566614/100‑1 (MQ=255)
aaTGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAggg < 2:775505/100‑1 (MQ=255)
tGATTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGAt < 2:1283646/100‑1 (MQ=255)
aTTGAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGa > 2:653236/1‑100 (MQ=255)
gAACAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGAATCTTCCTCGTCACCACGCTGTTAGGGATGAc > 2:273615/1‑98 (MQ=255)
aaCAGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCgg > 2:939612/1‑100 (MQ=255)
|
GCAATGGGTAGCGGCACAGACGTGGCGCTGGAAACCGCCGACGCAGCATTAACCCATAACCACCTGCGCGGCCTGGTGCAAATGATTGAACTGGCACGCGCCACTCACGCCAATATCCGCCAGAACATCACTATTGCGCTGGGGCTGAAAGGGATCTTCCTCGTCACCACGCTGTTAGGGATGACCGG > NC_000913/3608380‑3608567
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
