Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,639,576 |
(A)6→5 |
coding (145/336 nt) |
uspB ← |
universal stress (ethanol tolerance) protein B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,639,571 | 0 | A | . | 96.1%
| 216.1
/ 2.2
| 51 | coding (150/336 nt) | uspB | universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (22/27); total (23/28) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 |
ACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCA > NC_000913/3639472‑3639665
|
acaGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTa > 1:2583/1‑100 (MQ=255)
caGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaa > 2:1126525/1‑100 (MQ=255)
gcggcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTTGCCATGTGAGGTaaaa < 1:998877/100‑1 (MQ=255)
gcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagn < 1:537136/100‑3 (MQ=255)
gcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagc > 1:1203560/1‑98 (MQ=255)
cgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagcc > 1:1271534/1‑97 (MQ=255)
gAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagccc < 1:144295/100‑5 (MQ=255)
aaTTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTcc > 1:125761/1‑100 (MQ=255)
catcGTGATGATCGCGATAACGTTGGGCATTGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACa < 2:972515/100‑1 (MQ=255)
catcGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACATGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACa > 1:1249300/1‑100 (MQ=255)
gatgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa > 2:1019718/1‑100 (MQ=255)
atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGAt < 2:183352/100‑1 (MQ=255)
atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGAt < 2:395062/100‑1 (MQ=255)
tgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATa > 1:1287854/1‑100 (MQ=255)
gatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATag > 1:389101/1‑100 (MQ=255)
gcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCa > 2:1195264/1‑100 (MQ=255)
cGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATc < 1:68027/100‑1 (MQ=255)
tGGGGATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGc < 2:1146641/99‑1 (MQ=255)
tGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGc < 2:2583/100‑1 (MQ=255)
ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAg < 2:1083274/99‑1 (MQ=255)
ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGt > 1:177269/1‑100 (MQ=255)
cATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTa < 1:459236/100‑1 (MQ=255)
aGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg > 1:219678/1‑100 (MQ=255)
natatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg > 2:671678/2‑98 (MQ=255)
natatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa > 2:671693/2‑100 (MQ=255)
taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTAc < 2:1003021/100‑1 (MQ=255)
aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTAcc < 1:72758/100‑1 (MQ=255)
cAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCAc < 1:623325/100‑1 (MQ=255)
cAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCAc > 2:273684/1‑100 (MQ=255)
gagaCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaaca > 1:17624/1‑100 (MQ=255)
agaCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaa > 2:1170061/1‑100 (MQ=255)
agaCGCACCTGTTCGTTGGGTTGGCCATGTGAGGT‑AAAAAGCACCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaa > 1:882874/1‑100 (MQ=255)
aCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcg < 1:937333/100‑1 (MQ=255)
aCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcg < 2:1022368/100‑1 (MQ=255)
gCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg < 2:964685/100‑1 (MQ=255)
gCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg < 2:601985/100‑1 (MQ=255)
cACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgc > 1:244843/1‑100 (MQ=255)
cACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGCACCACTAACAAcgcgc > 2:760994/1‑100 (MQ=255)
tttgttGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtg < 2:882867/100‑1 (MQ=255)
tttgttGGGTTGGCCATGTGAAGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtg < 1:1081420/100‑1 (MQ=255)
ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAGCATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga > 2:714383/1‑100 (MQ=255)
ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAGCATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga > 2:714290/1‑100 (MQ=255)
ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga < 1:1245957/100‑1 (MQ=255)
ttgttgGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtga > 1:192702/1‑100 (MQ=255)
gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg < 1:1126535/100‑1 (MQ=255)
gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 1:833547/1‑100 (MQ=255)
gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg < 2:790544/100‑1 (MQ=255)
gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 2:676176/1‑100 (MQ=255)
gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 1:428157/1‑100 (MQ=255)
gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg > 1:689087/1‑100 (MQ=255)
gttgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgatg < 2:256090/100‑1 (MQ=255)
gggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATgaga < 1:1126000/100‑1 (MQ=255)
ttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAAt < 2:556367/100‑1 (MQ=255)
cATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc < 2:1271523/100‑1 (MQ=255)
cATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc < 1:747407/100‑1 (MQ=255)
tgtgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc < 2:1287843/100‑1 (MQ=255)
tgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa < 1:734510/100‑1 (MQ=255)
tgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa < 2:173395/100‑1 (MQ=255)
|
ACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCA > NC_000913/3639472‑3639665
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
