Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I1 R1	117	93.2	4682243	93.4%	4373214	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,852,356	T→A	L74Q (CTG→CAG)	hypD →	hydrogenase maturation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,852,356	0	T	A	92.5%	259.6 / 11.1	80	L74Q (CTG→CAG)	hypD	hydrogenase maturation protein
Reads supporting (aligned to +/- strand): ref base T (4/2); new base A (38/36); total (42/38)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

GTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAC  >  NC_000913/2852263‑2852451

gTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                           <  1:1054200/100‑1 (MQ=255)

gTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                           <  1:2074441/100‑1 (MQ=255)

gTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                           >  1:1716548/1‑100 (MQ=255)

 tCATTCCCACGCTGTCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                           <  2:1417757/99‑1 (MQ=255)

  cATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATg                                                                                          >  1:1390440/1‑99 (MQ=255)

   aTACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                        <  2:932397/100‑1 (MQ=255)

      cccACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAg                                                                                     <  1:592712/100‑1 (MQ=255)

         aCGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAAt                                                                                  <  2:1105617/100‑1 (MQ=255)

           gCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCg                                                                                <  1:1452367/100‑1 (MQ=255)

             tATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGac                                                                              <  2:914301/100‑1 (MQ=255)

              aTCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGaca                                                                             >  1:408955/1‑100 (MQ=255)

                cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGacatc                                                                           <  1:1576170/100‑3 (MQ=255)

                cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                           >  2:2165220/1‑100 (MQ=255)

                 tttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCt                                                                          >  2:2177825/1‑100 (MQ=255)

                  ttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTg                                                                         >  2:1894215/1‑100 (MQ=255)

                  ttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTg                                                                         >  2:1848562/1‑100 (MQ=255)

                  ttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTg                                                                         >  1:883985/1‑100 (MQ=255)

                    aaaTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCg                                                                       >  2:772755/1‑100 (MQ=255)

                      aTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTg                                                                     <  1:2157773/100‑1 (MQ=255)

                         cGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGgag                                                                  <  2:1799337/100‑1 (MQ=255)

                           gCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAt                                                                >  1:1430984/1‑100 (MQ=255)

                            ccTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGAtt                                                               >  1:419229/1‑100 (MQ=255)

                             cTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTGCAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTg                                                              <  2:1251441/100‑1 (MQ=255)

                             cTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTg                                                              <  2:1127538/100‑1 (MQ=255)

                               cGACCAGTTACTGGCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgcc                                                            <  1:2328331/100‑1 (MQ=255)

                               cGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgcc                                                            >  2:913377/1‑100 (MQ=255)

                                gACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgcca                                                           >  1:1171579/1‑100 (MQ=255)

                                 aCCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccag                                                          <  1:844213/100‑1 (MQ=255)

                                 aCCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccag                                                          <  1:1927964/100‑1 (MQ=255)

                                 aCCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccag                                                          >  2:2156189/1‑100 (MQ=255)

                                 aCCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccag                                                          <  1:1927902/100‑1 (MQ=255)

                                  ccAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagc                                                         <  1:834358/100‑1 (MQ=255)

                                   cAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagcc                                                        <  1:2177832/100‑1 (MQ=255)

                                    aGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagcca                                                       <  2:2199225/100‑1 (MQ=255)

                                    aGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagcca                                                       >  1:778204/1‑100 (MQ=255)

                                    aGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagcca                                                       <  2:28981/100‑1 (MQ=255)

                                       tACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATcc                                                    <  1:2088852/100‑1 (MQ=255)

                                       tACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATcc                                                    <  2:1156876/100‑1 (MQ=255)

                                         ntGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGAGTGCGTACAGCCGATGTGTAGAATCGCCACCTGCGTGTAGATTGCCAGCCATCCg                                                   >  2:149485/2‑99 (MQ=255)

                                         cTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCgg                                                  <  1:224478/100‑1 (MQ=255)

                                         cTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCgg                                                  >  2:2018281/1‑100 (MQ=255)

                                           gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGaa                                                >  1:1696792/1‑100 (MQ=255)

                                           gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGaa                                                <  2:1171576/100‑1 (MQ=255)

                                             cGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAgn                                              <  2:1972095/100‑2 (MQ=255)

                                             cGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGt                                              >  1:1336112/1‑100 (MQ=255)

                                             cGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGt                                              >  2:154288/1‑100 (MQ=255)

                                                 aaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtc                                          <  1:966278/100‑1 (MQ=255)

                                                    cGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtcttc                                       >  2:1183958/1‑100 (MQ=255)

                                                      ttGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTg                                     <  1:1102420/100‑1 (MQ=255)

                                                        gAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGGGATTGCCAGCCATCCGGAAGTCATCTTCTGTa                                   >  2:397787/1‑100 (MQ=255)

                                                         aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAc                                  <  2:1716543/100‑1 (MQ=255)

                                                         aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAc                                  >  2:819665/1‑100 (MQ=255)

                                                         aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAc                                  >  1:461562/1‑100 (MQ=255)

                                                         aGTTTATCCACCGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGCAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAc                                  >  2:1229440/1‑100 (MQ=255)

                                                          gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAcc                                 >  1:1508478/1‑100 (MQ=255)

                                                             tATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCttt                              >  1:47159/1‑100 (MQ=255)

                                                             tATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCttt                              >  2:1006060/1‑100 (MQ=255)

                                                             tATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCttt                              >  2:927754/1‑100 (MQ=255)

                                                               tCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTgg                            >  2:1174486/1‑100 (MQ=255)

                                                               tCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTgg                            >  2:753984/1‑100 (MQ=255)

                                                                ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                           >  2:701024/1‑100 (MQ=255)

                                                                ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                           >  1:1210568/1‑100 (MQ=255)

                                                                ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                           >  2:1319138/1‑100 (MQ=255)

                                                                ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                           >  1:1547499/1‑100 (MQ=255)

                                                                ccACGGTCCGGGGTGCCCGGTGTGAGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTgg                            >  1:145591/1‑99 (MQ=255)

                                                                    ggTCCGGGGTGCCCGGTGTGCGTACAGCCGATGTGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCTGGAAGTCATCTTCTGTACCTTTGGCGACg                       >  2:1402933/1‑100 (MQ=255)

                                                                    ggTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACg                       <  1:1006062/100‑1 (MQ=255)

                                                                    ggTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACg                       >  1:329197/1‑100 (MQ=255)

                                                                       ccGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCa                    <  1:1894222/100‑1 (MQ=255)

                                                                         ggggTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATg                  <  1:1174489/100‑1 (MQ=255)

                                                                          gggTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATgc                 <  1:701025/100‑1 (MQ=255)

                                                                             tGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGcgcg              <  2:883983/100‑1 (MQ=255)

                                                                              gCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGt             >  1:823319/1‑100 (MQ=255)

                                                                                  ggTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCg         >  1:1973506/1‑100 (MQ=255)

                                                                                   gtgtGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCgg        <  1:331862/100‑1 (MQ=255)

                                                                                    tgtgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCggg       <  1:2040129/100‑1 (MQ=255)

                                                                                      tgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGa     <  2:825543/100‑1 (MQ=255)

                                                                                       gCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaa    <  1:2018288/100‑1 (MQ=255)

                                                                                        cGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaaa   >  2:1562298/1‑100 (MQ=255)

                                                                                         gTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAc  >  1:66021/1‑100 (MQ=255)

GTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAC  >  NC_000913/2852263‑2852451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A