Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,852,356 T→A 100% L74Q (CTG→CAG)  hypD → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,852,3560TA98.8% 583.1 / ‑2.5 174L74Q (CTG→CAG) hypDhydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (98/73);  total (100/74)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

Only 100 of 174 total aligned reads displayed.

GGCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAC  >  NC_000913/2852259‑2852451
                                                                                                 |                                                                                               
ggCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGc                                                                                               <  1:1569162/100‑1 (MQ=255)
 gCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGcc                                                                                              <  2:2582116/100‑1 (MQ=255)
   ggTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTACCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGa                                                                                            >  1:1014110/1‑100 (MQ=255)
    gTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGCGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                           >  2:3096280/1‑100 (MQ=255)
    gTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                           <  1:543579/100‑1 (MQ=255)
    gTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                           <  1:232753/100‑1 (MQ=255)
     tCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATg                                                                                          <  1:3119977/100‑1 (MQ=255)
      cATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATgg                                                                                         >  2:3101030/1‑100 (MQ=255)
       aTACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                        <  1:346386/100‑1 (MQ=255)
       aTACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                        <  1:228721/100‑1 (MQ=255)
       aTACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                        <  2:2818698/100‑1 (MQ=255)
       aTACCCACGCTATCGTTAAAGTCGGCCTCGACCAGTTACTGCCGGAAAGCGTTACGTTTATCCACGGTCCGGGGTGCCCGGTGGGCGTACAGCCGATggg                                                                                        <  2:734222/100‑1 (MQ=255)
        tACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGt                                                                                       >  1:3595228/1‑100 (MQ=255)
               gCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCg                                                                                <  1:1705504/100‑1 (MQ=255)
                cTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGa                                                                               >  1:1803073/1‑100 (MQ=255)
                    cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                           <  1:3582189/100‑1 (MQ=255)
                    cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                           >  1:1938242/1‑100 (MQ=255)
                    cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                           >  1:3960259/1‑100 (MQ=255)
                     tttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                           <  2:2320266/99‑1 (MQ=255)
                     tttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCt                                                                          >  2:2918774/1‑100 (MQ=255)
                      ttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGacacaga                                                                         >  1:3002176/1‑97 (MQ=255)
                        aaaTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCg                                                                       <  2:1720417/100‑1 (MQ=255)
                          aTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTg                                                                     <  2:1475905/100‑1 (MQ=255)
                          aTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTg                                                                     >  2:3621819/1‑100 (MQ=255)
                          aTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTg                                                                     <  1:1394929/100‑1 (MQ=255)
                          aTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAATTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTg                                                                     >  1:2913321/1‑100 (MQ=255)
                            tCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGga                                                                   <  2:2087530/100‑1 (MQ=255)
                            tCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGga                                                                   <  2:1177785/100‑1 (MQ=255)
                            tCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGga                                                                   <  1:573145/100‑1 (MQ=255)
                               gCCTCGCCCAGTTACTGCCGGAAAACGTTGAGTTTATCCTCGGTGCGGGGTGCGCGGTGTGCGTACAGCCGATGGGTAGGATCGACACCTGCGTgg                                                                    <  2:2882968/96‑1 (MQ=255)
                               gCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAt                                                                >  1:1235997/1‑100 (MQ=255)
                               gCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAt                                                                >  1:1515511/1‑100 (MQ=255)
                                ccTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAtt                                                               >  1:1958009/1‑100 (MQ=255)
                                ccTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAtt                                                               >  1:3486630/1‑100 (MQ=255)
                                ccTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAtt                                                               >  1:3378185/1‑100 (MQ=255)
                                   cGACCAGTTACTGCCGGAAAACGTTTAGTTTATCCACGGTCCGGGGTGCCCCGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgcc                                                            <  2:1806954/100‑1 (MQ=255)
                                      cgaGTTACTGCCGGAAAACGTGGCGTTAATCCACGGTCCGCGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGCCACCTGCGTGGAGATTgccag                                                          <  2:208759/97‑1 (MQ=255)
                                      ccAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagc                                                         <  1:987607/100‑1 (MQ=255)
                                         gTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCAt                                                      >  2:453828/1‑100 (MQ=255)
                                             cTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCgg                                                  >  2:3018165/1‑100 (MQ=255)
                                              tGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGa                                                 >  2:3951729/1‑100 (MQ=255)
                                               gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagcca                                                       >  2:643020/1‑93 (MQ=255)
                                               gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGaa                                                >  1:3717154/1‑100 (MQ=255)
                                               gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGaa                                                >  2:3784449/1‑100 (MQ=255)
                                               gCCGGAAAACGTTGAGGTTATCCAGGGTCCGGGGTGCGCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCg                                                   >  2:1069043/1‑97 (MQ=255)
                                               gCCGGAAAACGTAGAGTTTATCCACGGTCCGGGGTGCCCGGTCTGCGTAAAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCCGaa                                                >  2:1201437/1‑100 (MQ=255)
                                                ccGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGaan                                               <  1:3411160/100‑2 (MQ=255)
                                                ccGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAg                                               <  2:2076248/100‑1 (MQ=255)
                                                 cGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGt                                              >  1:1982455/1‑100 (MQ=255)
                                                  ggAAAACGTTGAGTTTATCCACGGTCTGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTc                                             >  1:2518709/1‑100 (MQ=255)
                                                  ggAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGt                                              >  2:1153906/1‑99 (MQ=255)
                                                  ggAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTc                                             >  2:2138703/1‑100 (MQ=255)
                                                  ggAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTc                                             >  1:825005/1‑100 (MQ=255)
                                                   gAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCa                                            >  1:292497/1‑100 (MQ=255)
                                                    aaaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAt                                           >  2:1291804/1‑100 (MQ=255)
                                                    aaaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAt                                           >  1:1322265/1‑100 (MQ=255)
                                                      aaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtct                                         >  2:3697131/1‑100 (MQ=255)
                                                       aCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtctt                                        >  2:2738645/1‑100 (MQ=255)
                                                         gTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAACCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtcttct                                      <  1:1888883/100‑1 (MQ=255)
                                                          ttaaGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTg                                     >  1:459579/4‑100 (MQ=255)
                                                          ttGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGCTTGCCAGCCCTCCGGAAGTCAtcttc                                       >  2:813701/1‑98 (MQ=255)
                                                          ttGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCCACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTg                                     >  2:2641537/1‑100 (MQ=255)
                                                           tGAGTTTATCCACGGTCCGGGGTGCCCGGTGGGCGTACGGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTg                                     <  2:1036689/99‑1 (MQ=255)
                                                            gAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTa                                   >  2:3206252/1‑100 (MQ=255)
                                                             aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAGTCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTa                                   >  2:3452254/1‑99 (MQ=255)
                                                             aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAc                                  >  1:3271651/1‑100 (MQ=255)
                                                             aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAc                                  <  2:4064680/100‑1 (MQ=255)
                                                              gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAcc                                 >  1:1744848/1‑100 (MQ=255)
                                                              gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAc                                  >  2:1588423/1‑99 (MQ=255)
                                                              gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCGTCTGTa                                   >  1:1419859/1‑98 (MQ=255)
                                                                ttATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCtt                               <  1:2532174/100‑1 (MQ=255)
                                                                 tATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCttt                              <  1:2741004/100‑1 (MQ=255)
                                                                   tCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTgg                            >  2:3136444/1‑100 (MQ=255)
                                                                    ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                           >  1:2575639/1‑100 (MQ=255)
                                                                    ccACGGTCCGGCGTGCCCGGTGTGCGTAAAGCCTATGGGTAGAATCGACACCTGCGTGGATATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                           <  2:3460179/100‑1 (MQ=255)
                                                                     cACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCg                          >  1:2665973/1‑100 (MQ=255)
                                                                     cACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCg                          >  2:2956564/1‑100 (MQ=255)
                                                                     cACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCg                          <  2:4008481/100‑1 (MQ=255)
                                                                       cGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGAc                        <  1:3705515/100‑1 (MQ=255)
                                                                          tCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGcc                     <  1:71234/100‑1 (MQ=255)
                                                                           ccGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCa                    >  2:1692538/1‑100 (MQ=255)
                                                                            cGGGGTGCCCGGTGTGCGTACAGCTGAGGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCa                    <  2:3378165/99‑1 (MQ=255)
                                                                                gTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATgcgc               <  1:1095306/100‑1 (MQ=255)
                                                                                 tGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGcgcg              <  2:3595205/100‑1 (MQ=255)
                                                                                  gCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGt             >  2:2933959/1‑100 (MQ=255)
                                                                                   cccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGCCGCCATGCGCGTg            >  2:3342850/1‑100 (MQ=255)
                                                                                   cccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGt             >  2:16866/1‑99 (MQ=255)
                                                                                    ccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGc           >  2:3053424/1‑100 (MQ=255)
                                                                                     cGGGGTGCGTACAGCCGATGGGGAGGATCGACGCGTGCGGGGAGATTGCCAGCCATCCGGAAGACATTTTGTGTACCTTTGGGGAAGCCATGTGGGTGcc          <  2:602896/100‑1 (MQ=255)
                                                                                        tgtgCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCgg        >  1:2434093/1‑99 (MQ=255)
                                                                                        tgtgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCggg       <  1:1235268/100‑1 (MQ=255)
                                                                                         gtgCGTACAGCCGATGGGTAGAATCGACCCCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCAACGCCATGCGCGTGCCgggg      <  2:2532703/100‑1 (MQ=255)
                                                                                         gtgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCgggg      >  1:1677945/1‑100 (MQ=255)
                                                                                         gtgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCgggg      <  1:226389/100‑1 (MQ=255)
                                                                                           gCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaa    <  1:2690326/100‑1 (MQ=255)
                                                                                           gCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaa    >  1:3217952/1‑100 (MQ=255)
                                                                                            cGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaaa   >  1:1001801/1‑100 (MQ=255)
                                                                                            cGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaaa   >  1:1248969/1‑100 (MQ=255)
                                                                                             gTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAc  <  2:1874000/100‑1 (MQ=255)
                                                                                             gTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAc  <  1:3245825/100‑1 (MQ=255)
                                                                                                 |                                                                                               
GGCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAC  >  NC_000913/2852259‑2852451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A