Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F256 I0 R1	328	102.7	5123138	93.4%	4785010	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,852,356	T→A	100%	L74Q (CTG→CAG)	hypD →	hydrogenase maturation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,852,356	0	T	A	98.8%	282.0 / ‑4.5	85	L74Q (CTG→CAG)	hypD	hydrogenase maturation protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (57/27); total (57/28)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAACA  >  NC_000913/2852260‑2852452

gCGGTCATACCCTCGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGcc                                                                                               <  1:89402/100‑1 (MQ=255)

   gTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGAt                                                                                            <  2:2001381/100‑1 (MQ=255)

    tCATACCAACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATg                                                                                           <  2:1243168/100‑1 (MQ=255)

     cATACCCACGCTATCGTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGCGTGCGTACAGCCGATg                                                                                           >  2:2319421/1‑99 (MQ=255)

      aTACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGTCCGGTGTGCGTACAGCCGATggg                                                                                         <  1:128202/100‑1 (MQ=255)

      aTACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                         <  1:2326396/100‑1 (MQ=255)

      aTACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                         <  1:467492/100‑1 (MQ=255)

       tACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATggg                                                                                         >  2:2323227/1‑99 (MQ=255)

        aCCCCCGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGGGTTTATCCAGGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTa                                                                                       <  2:798422/100‑1 (MQ=255)

        aCCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTa                                                                                       <  1:1246697/100‑1 (MQ=255)

         cccACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTa                                                                                       <  2:727434/99‑1 (MQ=255)

         cccACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAg                                                                                      >  2:1770042/1‑100 (MQ=255)

         cccACGCTATCTTTAAATTCGGCCTCGACCAGATACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAg                                                                                      >  1:2431034/1‑100 (MQ=255)

             cGCTATCTTTAAATTCGGCCTCGACCAGTCACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGCTGCCCGGTGTGCGTACAGCCGATGGGTAGAATc                                                                                  <  2:1535680/100‑1 (MQ=255)

               cTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGTCCGGTGTGTGTACAGCCGATGGGTAGAATCGa                                                                                >  2:140828/1‑100 (MQ=255)

                tATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGac                                                                               >  1:857269/1‑100 (MQ=255)

                  tCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGATGTGCGTACAGCCGATGGGTAGAATCGacac                                                                             >  2:75246/1‑100 (MQ=255)

                   cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                            >  2:630762/1‑100 (MQ=255)

                   cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                            <  1:1070756/100‑1 (MQ=255)

                   cTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                            >  1:2312066/1‑100 (MQ=255)

                   cTTTAAATTCGGCCTCAACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGGGCGTACAGCCGATGGGTAGAATCGACAcc                                                                            >  2:608460/1‑100 (MQ=255)

                    tttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCAGTGTGCGTACAGCCGATGGGTAGAATCGACACCt                                                                           >  2:1350360/1‑100 (MQ=255)

                    nttAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTANCCACNGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACAcc                                                                            >  1:1223125/2‑99 (MQ=255)

                      tAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGc                                                                         >  2:1502404/1‑100 (MQ=255)

                       aaaTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCg                                                                        >  1:302051/1‑100 (MQ=255)

                        caTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGt                                                                       >  1:416569/2‑100 (MQ=255)

                        aaTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGt                                                                       >  1:1692007/1‑100 (MQ=255)

                         aTTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTg                                                                      >  2:1002334/1‑100 (MQ=255)

                          ttCGGCCTCGATGGGGTACTGCCGGAAAACGGTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTg                                                                      <  2:287466/99‑1 (MQ=255)

                          ttCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTgg                                                                     <  1:315732/100‑1 (MQ=255)

                            cGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGgag                                                                   <  1:624550/100‑1 (MQ=255)

                              gCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAt                                                                 >  1:903122/1‑100 (MQ=255)

                               ccTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAtt                                                                >  2:1825181/1‑100 (MQ=255)

                               ccTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGAtt                                                                >  1:2282034/1‑100 (MQ=255)

                                 tCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgc                                                              >  1:2838/1‑100 (MQ=255)

                                  cGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgcc                                                             >  2:108606/1‑100 (MQ=255)

                                   gACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTATAGCCGAAGGGTAGAATCGACACCTGCGTGGAGATTgcca                                                            >  2:1799878/1‑100 (MQ=255)

                                    aCCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccag                                                           <  1:2061733/100‑1 (MQ=255)

                                     ccAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagc                                                          >  1:512271/1‑100 (MQ=255)

                                      cAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTgccagcc                                                         >  2:710086/1‑100 (MQ=255)

                                           aCTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCg                                                    >  1:1825859/1‑100 (MQ=255)

                                              gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATTGGAACCTGCGTGGAGATTGCCAGCCATCCAGaa                                                 >  1:199658/1‑100 (MQ=255)

                                              gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGaa                                                 >  2:323658/1‑100 (MQ=255)

                                              gCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGa                                                  >  2:328287/1‑99 (MQ=255)

                                               ccGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAg                                                >  2:1495143/1‑100 (MQ=255)

                                                 ggAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTc                                              >  1:880767/1‑100 (MQ=255)

                                                   aaaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAt                                            >  2:1208873/1‑100 (MQ=255)

                                                      aCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtctt                                         >  2:1160009/1‑100 (MQ=255)

                                                       cGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtcttc                                        >  1:1980908/1‑100 (MQ=255)

                                                       cGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAtcttc                                        >  1:1442721/1‑100 (MQ=255)

                                                        gTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCCTCCGGAAGTCAtctt                                         >  2:906286/1‑98 (MQ=255)

                                                         ttGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTg                                      >  1:777833/1‑100 (MQ=255)

                                                           gAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTa                                    <  1:72457/100‑1 (MQ=255)

                                                             gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAcc                                  >  1:329264/1‑100 (MQ=255)

                                                             gTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTAcc                                  >  1:1453682/1‑100 (MQ=255)

                                                              tttATCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAAGCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCt                                 <  2:230741/100‑1 (MQ=255)

                                                                 aTCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTg                              >  2:2469643/1‑100 (MQ=255)

                                                                 aTCCACGGTCCGGGGTACCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTg                              <  1:1904757/100‑1 (MQ=255)

                                                                  tCCACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGACTCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCCTCTTCTGTACCttt                               >  2:1093676/1‑98 (MQ=255)

                                                                   ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                            >  2:956254/1‑100 (MQ=255)

                                                                   ccACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGc                            <  2:186602/100‑1 (MQ=255)

                                                                    cACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACCCCTGCGTGGAGATTGCCAGCCAGCCGGAAGTCATCTTCTGTACCTTTGGCg                           >  2:1768429/1‑100 (MQ=255)

                                                                    cACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCg                           >  1:2149728/1‑100 (MQ=255)

                                                                    cACGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCg                           >  2:2535447/1‑100 (MQ=255)

                                                                      cGGTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGAc                         <  2:1430809/100‑1 (MQ=255)

                                                                       ggTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACg                        >  2:1182018/1‑100 (MQ=255)

                                                                       ggTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACg                        >  2:244496/1‑100 (MQ=255)

                                                                        gTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGc                       >  1:164912/1‑100 (MQ=255)

                                                                        gTCCGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGc                       >  1:1213991/1‑100 (MQ=255)

                                                                         tCCGGGGTGCCCGGTGTGCGGACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGcc                      <  2:256560/100‑1 (MQ=255)

                                                                          ccGGGGTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGGGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCa                     >  1:1191961/1‑100 (MQ=255)

                                                                             gggTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATgc                  >  1:950242/1‑100 (MQ=255)

                                                                              ggTGCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATgcg                 <  1:57428/100‑1 (MQ=255)

                                                                                 gCCCGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGcgcg               <  2:2125301/99‑1 (MQ=255)

                                                                                  cccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTg             >  2:1616653/1‑100 (MQ=255)

                                                                                  cccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTg             <  2:2126596/100‑1 (MQ=255)

                                                                                  cccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTg             <  1:144063/100‑1 (MQ=255)

                                                                                   ccGGTGTGCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGc            <  2:806108/100‑1 (MQ=255)

                                                                                         tgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACGTTTGGCGACGCCATGGGGGTGCCGGGGa      >  2:467082/1‑100 (MQ=255)

                                                                                         tgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGa      >  1:306299/1‑100 (MQ=255)

                                                                                         tgCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGa      <  1:257324/100‑1 (MQ=255)

                                                                                          gCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTTTACCTTTGGCGACGCCATGCGCGTGCCGGGGaa     <  2:1670183/100‑1 (MQ=255)

                                                                                          gCGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaa     >  1:1229414/1‑100 (MQ=255)

                                                                                           cGTACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGaaa    >  1:556349/1‑100 (MQ=255)

                                                                                             tACAGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCACGTGCCGGGGAAACa  >  1:2525526/1‑100 (MQ=255)

GCGGTCATACCCACGCTATCTTTAAATTCGGCCTCGACCAGTTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAACA  >  NC_000913/2852260‑2852452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A