Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	14	51.3	1601205	98.8%	1581990	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,152	T→G	L268R (CTG→CGG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,152	0	T	G	100.0%	156.1 / NA	41	L268R (CTG→CGG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (13/28); total (13/28)

ATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGA  >  NC_000913/4186010‑4186295

aTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTAtcgtc                                                                                                                                         <  1:28761/151‑1 (MQ=255)

        gctgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCAt                                                                                                                                  <  2:33204/150‑1 (MQ=255)

              aGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGt                                                                                                                           <  1:131548/151‑1 (MQ=255)

                  ttcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaac                                                                                                                        <  1:425581/150‑1 (MQ=255)

                  ttcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTa                                                                                                                          >  1:420754/1‑148 (MQ=255)

                   tcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaaca                                                                                                                       <  2:239456/150‑1 (MQ=255)

                    cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaacaac                                                                                                                     <  1:673007/151‑1 (MQ=255)

                    cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaacaa                                                                                                                      <  1:411575/150‑1 (MQ=255)

                        cAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTc                                                                                                                 <  2:181943/151‑1 (MQ=255)

                          gTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTg                                                                                                               <  1:37163/151‑1 (MQ=255)

                             tGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGaa                                                                                                             >  2:347772/1‑150 (MQ=255)

                                       ccAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTgg                                                                                                  <  2:627874/151‑1 (MQ=255)

                                          gagTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATc                                                                                               <  2:653634/151‑1 (MQ=255)

                                              ggATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTgg                                                                                            <  2:547506/150‑1 (MQ=255)

                                              ggATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGc                                                                                           <  2:480462/151‑1 (MQ=255)

                                              ggATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGc                                                                                           <  2:104691/151‑1 (MQ=255)

                                                  gatCCTGACCGTTCTGCCGGTACTGCCGCCATATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCCGGATCTGGCTgcg                                                                                       <  1:180409/151‑1 (MQ=255)

                                                  gatCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTgcg                                                                                       >  2:787483/1‑151 (MQ=255)

                                                       tGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCgg                                                                                   >  2:167838/1‑150 (MQ=255)

                                                       tGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCgg                                                                                   >  1:179577/1‑150 (MQ=255)

                                                        gACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAc                                                                                 <  1:179654/151‑1 (MQ=255)

                                                                  ccGGTACTTCCGCCAGATCTACCTCCGCTGGTTCCGCTGAATGGTTGTCGTTTCGCGACTTCTGACCTGAACGGTGGGTGTCGGCTCGGCATTAACCGTAACAACCGTCTGATACGTCTGCTGGATCTGTCTGCTCCGGGca                                                                                <  1:623269/142‑1 (MQ=255)

                                                                  ccGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgt                                                                         <  1:787398/149‑1 (MQ=255)

                                                                   cGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgtacg                                                                      <  2:267493/151‑1 (MQ=255)

                                                                       aCTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAAc                                                                  <  2:564633/151‑1 (MQ=255)

                                                                           ccgccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaa                                                               >  1:677219/1‑150 (MQ=255)

                                                                            cgccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaaa                                                             <  1:266167/151‑1 (MQ=255)

                                                                               cAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGGCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACg                                                          <  2:691335/151‑1 (MQ=255)

                                                                                aGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGt                                                         <  1:722136/151‑1 (MQ=255)

                                                                                aGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGt                                                         >  1:125173/1‑151 (MQ=255)

                                                                                              tGGTTCCGCTGGCTGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGCATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGCCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                           >  1:75592/1‑151 (MQ=255)

                                                                                                   ccGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAg                                      <  1:306407/151‑1 (MQ=255)

                                                                                                    cGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGt                                        >  2:347351/1‑148 (MQ=255)

                                                                                                     gCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGAc                                    <  2:387759/151‑1 (MQ=255)

                                                                                                           tggtggTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCctg                              >  2:313020/1‑151 (MQ=255)

                                                                                                            ggtggtCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCctgc                             <  2:705708/151‑1 (MQ=255)

                                                                                                            ggtggtCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCctg                              <  2:423761/150‑1 (MQ=255)

                                                                                                                   gTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATa                       >  2:720640/1‑150 (MQ=255)

                                                                                                                                tGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGt         >  1:189449/1‑151 (MQ=255)

                                                                                                                                 gACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTc        >  2:269766/1‑151 (MQ=255)

                                                                                                                                       aaCGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGa  <  2:593550/151‑1 (MQ=255)

ATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGA  >  NC_000913/4186010‑4186295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A