Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I3 R1	9	53.9	1676598	99.1%	1661508	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,274	A→T	N309Y (AAC→TAC)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,274	0	A	T	100.0%	181.1 / NA	46	N309Y (AAC→TAC)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (24/22); total (24/22)

CGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATC  >  NC_000913/4186129‑4186414

cgcgACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGgt                                                                                                                                         >  1:283032/1‑151 (MQ=255)

 gcgACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGgtc                                                                                                                                        <  2:735541/151‑1 (MQ=255)

  cgACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGgtcg                                                                                                                                       <  2:762528/151‑1 (MQ=255)

     cTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGtcgtcg                                                                                                                                    >  1:544042/1‑151 (MQ=255)

        cTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCgg                                                                                                                                 >  2:403470/1‑151 (MQ=255)

               gAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGc                                                                                                                           <  2:246753/150‑1 (MQ=255)

               gAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGc                                                                                                                           <  2:459053/150‑1 (MQ=255)

                        gTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCAc                                                                                                                     <  1:136092/147‑1 (MQ=255)

                        gTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGt                                                                                                                 <  1:608235/151‑1 (MQ=255)

                           tcgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCa                                                                                                                      <  2:279802/143‑1 (MQ=255)

                            cgtcgCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCt                                                                                                              >  1:622529/1‑150 (MQ=255)

                               cgcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAAc                                                                                                           >  1:170426/1‑150 (MQ=255)

                                gcgTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACa                                                                                                          >  2:481020/1‑150 (MQ=255)

                                     aTTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTc                                                                                                    <  1:451151/151‑1 (MQ=255)

                                          ccGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGCAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTg                                                                                               >  2:623026/1‑151 (MQ=255)

                                             tAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGaa                                                                                             >  2:190456/1‑150 (MQ=255)

                                              aacaacCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAAt                                                                                           <  2:378003/151‑1 (MQ=255)

                                                   ccGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTg                                                                                      <  2:27151/151‑1 (MQ=255)

                                                         gAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGcc                                                                                   <  1:793373/148‑1 (MQ=255)

                                                            aCGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATg                                                                             <  2:101512/151‑1 (MQ=255)

                                                                 tgctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCa                                                                         >  1:156650/1‑150 (MQ=255)

                                                                   ctgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCaa                                                                        >  1:559174/1‑149 (MQ=255)

                                                                    tgGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                                                     >  2:413375/1‑151 (MQ=255)

                                                                     ggATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGt                                                                    <  1:796309/151‑1 (MQ=255)

                                                                      gATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAgg                                                                     >  1:613584/1‑149 (MQ=255)

                                                                               gcgcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAggg                                                            <  1:817538/149‑1 (MQ=255)

                                                                                 gcCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCg                                                         <  2:597995/150‑1 (MQ=255)

                                                                                     gACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTcc                                                    >  1:637786/1‑151 (MQ=255)

                                                                                           atcGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTc                                                 >  2:823495/1‑148 (MQ=255)

                                                                                            tcGTACGTAACGAAAAACGTATGCTGCCGGAAGCGGTAGTCGCCCTGCGGGATTACGGTCGTCGCGGTCGTGTGACCACCGGTTCTAACAAGCGTCCTCTGATATCTTTGGCCAACATGATCAAAGTTAAACAGGGTAGATTCCGTCAGa                                              <  1:679634/150‑1 (MQ=255)

                                                                                             cgtacgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAAc                                            >  2:144649/1‑151 (MQ=255)

                                                                                                acgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCt                                          <  2:651861/150‑1 (MQ=255)

                                                                                                acgtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTg                                         >  2:463068/1‑151 (MQ=255)

                                                                                                  gtaACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGc                                        <  1:69693/150‑1 (MQ=255)

                                                                                                          aaaCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGc                               <  1:452261/151‑1 (MQ=255)

                                                                                                            aCGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgt                             >  2:202081/1‑151 (MQ=255)

                                                                                                              gTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCgtgt                           >  1:154816/1‑151 (MQ=255)

                                                                                                                   ctgcAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGact                      >  1:108101/1‑151 (MQ=255)

                                                                                                                      cAGGAAGCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCCGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGactact                   >  1:539428/1‑151 (MQ=255)

                                                                                                                            gCGGTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTc             <  2:688873/151‑1 (MQ=255)

                                                                                                                               gTAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCGTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGGCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGtt          <  1:191591/151‑1 (MQ=255)

                                                                                                                                tAGACGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTc         >  1:543245/1‑151 (MQ=255)

                                                                                                                                   aCGCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGt      <  2:346206/151‑1 (MQ=255)

                                                                                                                                     gCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa     >  1:710811/1‑150 (MQ=255)

                                                                                                                                     gCCCTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTa     >  1:744797/1‑150 (MQ=255)

                                                                                                                                       ccTGCTGGATTACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATc  >  2:363342/1‑151 (MQ=255)

CGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATCACCGGTTCTAACAAGCGTCCTCTGAAATCTTTGGCCGACATGATCAAAGGTAAACAGGGTCGTTTCCGTCAGAACCTGCTCGGTAAGCGTGTTGACTACTCCGGTCGTTCTGTAATC  >  NC_000913/4186129‑4186414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A