Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I1 R1	16	44.5	1394873	98.4%	1372555	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,152	T→G	L268R (CTG→CGG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,152	0	T	G	100.0%	176.3 / NA	45	L268R (CTG→CGG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (18/27); total (18/27)

AAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAT  >  NC_000913/4186013‑4186296

aaaCTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTcgcg                                                                                                                                       <  2:433015/151‑1 (MQ=255)

        ggAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAAc                                                                                                                               >  1:310023/1‑151 (MQ=255)

                tcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaaca                                                                                                                        <  2:48535/150‑1 (MQ=255)

                tcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaaca                                                                                                                        <  1:396376/150‑1 (MQ=255)

                 cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaacaac                                                                                                                      <  2:655092/151‑1 (MQ=255)

                 cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaacaac                                                                                                                      <  2:36766/151‑1 (MQ=255)

                             tAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGt                                                                                                          >  1:405531/1‑151 (MQ=255)

                              aaCAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGt                                                                                                          <  2:504652/150‑1 (MQ=255)

                                      agagTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGAt                                                                                                 <  2:290881/151‑1 (MQ=255)

                                      agagTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGAt                                                                                                 <  1:76088/151‑1 (MQ=255)

                                               gatCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTgcg                                                                                        >  2:199882/1‑151 (MQ=255)

                                                    tGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCgg                                                                                    >  1:657614/1‑150 (MQ=255)

                                                     gACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAc                                                                                  >  2:449823/1‑151 (MQ=255)

                                                         gTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAcatc                                                                               >  2:646882/1‑150 (MQ=255)

                                                              gCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgt                                                                          >  2:15980/1‑150 (MQ=255)

                                                              gCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcg                                                                           <  2:53683/149‑1 (MQ=255)

                                                                  gTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgtacgta                                                                     <  2:17300/151‑1 (MQ=255)

                                                                       gccgccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACg                                                                  <  1:348019/149‑1 (MQ=255)

                                                                         cgccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACTTATGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaaa                                                              <  1:6474/151‑1 (MQ=255)

                                                                         ccccAGATCTGCGTCCGGTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATGGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaaa                                                              <  1:199934/149‑1 (MQ=255)

                                                                            cAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACg                                                           >  1:556238/1‑151 (MQ=255)

                                                                             aGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGt                                                          >  2:506794/1‑151 (MQ=255)

                                                                                 cTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGATATCATCGTACGTAACGAAAAACGTAtgc                                                      >  1:659079/1‑151 (MQ=255)

                                                                                   gCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTAtgctg                                                    <  1:556428/151‑1 (MQ=255)

                                                                                    cGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTAtgctgc                                                   <  2:579454/151‑1 (MQ=255)

                                                                                           tGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                            >  2:142335/1‑151 (MQ=255)

                                                                                            ggTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                            <  1:14264/150‑1 (MQ=255)

                                                                                            ggTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                            <  1:244757/150‑1 (MQ=255)

                                                                                            ggTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCg                                           <  2:294274/151‑1 (MQ=255)

                                                                                                ccGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTa                                        <  2:117970/150‑1 (MQ=255)

                                                                                                ccGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAg                                       <  2:515023/151‑1 (MQ=255)

                                                                                                ccGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAg                                       <  1:499235/151‑1 (MQ=255)

                                                                                                      gATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGccc                                 >  2:239251/1‑151 (MQ=255)

                                                                                                              tCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGAt                         >  2:396380/1‑151 (MQ=255)

                                                                                                                    cgcgACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGgt                   <  1:116730/151‑1 (MQ=255)

                                                                                                                    cccgACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGgt                   >  1:144733/3‑151 (MQ=255)

                                                                                                                      cgACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGgtcg                 >  1:299851/1‑151 (MQ=255)

                                                                                                                           tCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAAACGCCCTGCTGGATAACGGTCGTcgcg            >  1:172536/1‑151 (MQ=255)

                                                                                                                              gACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTc         <  2:488891/151‑1 (MQ=255)

                                                                                                                                ccTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGt       <  1:685119/151‑1 (MQ=255)

                                                                                                                                ccTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGt       >  1:91358/1‑151 (MQ=255)

                                                                                                                                   gAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTg      <  2:556275/149‑1 (MQ=255)

                                                                                                                                   gAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGc     <  1:646834/150‑1 (MQ=255)

                                                                                                                                    aaCGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGc     <  2:495768/149‑1 (MQ=255)

                                                                                                                                     aCGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAt  >  2:62079/1‑151 (MQ=255)

AAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGAT  >  NC_000913/4186013‑4186296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A