Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,538,396 A→G T338A (ACT→GCT)  yhgF → putative transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,538,3960AG100.0% 185.0 / NA 52T338A (ACT→GCT) yhgFputative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

GCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGT  >  NC_000913/3538251‑3538532
                                                                                                                                                 |                                                                                                                                          
gCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTggg                                                                                                                                       <  1:287800/151‑1 (MQ=255)
gCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTgg                                                                                                                                        >  2:439788/1‑150 (MQ=255)
   tCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGt                                                                                                                                     >  1:457494/1‑150 (MQ=255)
     tGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTaa                                                                                                                                   >  2:110690/1‑150 (MQ=255)
       gAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTaaa                                                                                                                                  >  2:12468/1‑149 (MQ=255)
                 tGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCggtg                                                                                                                        >  1:219942/1‑149 (MQ=255)
                  gATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCggtggt                                                                                                                      <  1:69631/150‑1 (MQ=255)
                      ggCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATg                                                                                                                 <  1:887565/151‑1 (MQ=255)
                           cGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCggtggggggaa                                                                                                                 >  2:458758/1‑140 (MQ=255)
                            gTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTg                                                                                                          <  1:265076/151‑1 (MQ=255)
                                     cGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACtgg                                                                                                 <  2:219085/151‑1 (MQ=255)
                                      gTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGATGCC‑CCCGGGaaa                                                                                                     >  2:128480/1‑146 (MQ=255)
                                          ggAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGATGCC‑CCTGGCAAACtggggggg                                                                                            >  1:796942/1‑149 (MQ=255)
                                               aTGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGAGGCC‑ACTCGCAAACtggtg                                                                                               >  1:831084/1‑143 (MQ=255)
                                                tGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGGTGCC‑ACTTGCAAACTGGTGGcg                                                                                            >  1:145274/1‑145 (MQ=255)
                                                 gAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGac‑a                                                                                    <  1:663440/151‑1 (MQ=255)
                                                   aGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACCGGCCAACAGGGGGGGACCGAC‑Acc                                                                                  >  1:886634/1‑151 (MQ=255)
                                                    gCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑Acc                                                                                  <  2:773714/150‑1 (MQ=255)
                                                    gCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGTGGTGCC‑CCTGGCCAACCTGTGGggaccggc                                                                                      >  2:369240/1‑145 (MQ=255)
                                                     cAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGGTGCC‑ACTGGCAAACTGGTGGGGCCCGCC‑Acc                                                                                  >  1:555646/1‑149 (MQ=255)
                                                      aaTCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGGGGGCGGGGGC‑CCTGGGAAACTGGGGGGGGCCGCC‑Acc                                                                                  >  2:86691/1‑148 (MQ=255)
                                                          aaCGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGGGGGCGGTGCC‑CCTGGGAAACCGGGGGCGGCCCCC‑Acccatt                                                                              >  2:714433/1‑144 (MQ=255)
                                                                 ttGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGcac                                                                      <  1:41751/149‑1 (MQ=255)
                                                                   gCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGCTGACAGTGCC‑CCTGGGAAACTGGGGGCGCGCGACAACCATTAAccacacccc                                                                    >  1:703386/1‑143 (MQ=255)
                                                                      cGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGGGGCCTCCCGGCAAACGGGGGGGGACCG‑C‑ACCAtttttccccccccc                                                                   >  2:219043/1‑136 (MQ=255)
                                                                       gTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGATGCC‑ACCTGCAAACTGGTGGGGGCCGGC‑ACCCTTTTCCCGCACAcc                                                                   >  1:145642/1‑146 (MQ=255)
                                                                       gTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGCGTAAAAGTGGCGGTGGGCGGTGGC‑CCACGCAAAACGGTTGCGACCGAC‑ACCACTTATCCGcccgcccgcc                                                               >  2:440887/1‑141 (MQ=255)
                                                                         aaCCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAgg                                                            <  2:664538/151‑1 (MQ=255)
                                                                              gCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCa                                                       <  2:457447/151‑1 (MQ=255)
                                                                                       gctgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCaaaa                                                    <  2:839967/145‑1 (MQ=255)
                                                                                       gctgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAagcagc                                               <  2:395999/150‑1 (MQ=255)
                                                                                         tgATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGGTGCC‑CCCGGCCAACAGGGGGGGGCCGAC‑ACCATTTTCCCGCCCCCCggggcgggcgccaaaa                                                   >  2:826000/1‑130 (MQ=255)
                                                                                          gATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACCCCGGACAGGCCGCAAAAGCAGCGATg                                           <  2:389752/151‑1 (MQ=255)
                                                                                                    ccTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGACGATGCC‑ACTTGCAAAATGGTGGCGACCGAC‑AACCTTTACCCGCCCACCCGGCAGGCCCCAAAAACAGCGATGaac                                        >  1:883498/1‑142 (MQ=255)
                                                                                                       gCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTtgctgc                                <  2:184700/149‑1 (MQ=255)
                                                                                                       gCCGGACGGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACGGGTGGCGACCGAC‑ACCATTTCCCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTtgctgc                                <  2:880360/149‑1 (MQ=255)
                                                                                                         cGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑CCTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAACAGCGATGACCggt                                     >  1:544675/1‑140 (MQ=255)
                                                                                                         cGGACTGCGTGCAACGATGGGCCGCGATCCGGGTCGGCGTGCTGGGGTAAAAGTGGCGGGGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCt                             <  2:410057/150‑1 (MQ=255)
                                                                                                          ggACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgt                           <  1:447053/151‑1 (MQ=255)
                                                                                                            aCTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgtgt                         <  2:260028/151‑1 (MQ=255)
                                                                                                            aCTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGGCGGTGCC‑ACTGGCAAACTGGTGGGGCCCGGC‑ACCACTTAACCGCACACCCGACAGGCCGCCAAAACAGCGCGGACCCTTGGTGCGCtgtg                          >  2:150597/1‑150 (MQ=255)
                                                                                                                cGTGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgtgtg                        <  2:428549/148‑1 (MQ=255)
                                                                                                                  tGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑CCTGGCAAACTGGTGGCGGCCGAC‑ACCCATTAACCGCCCACCGGGCAGGCCGCAAAAACAGCGGTGACCGTTGCTGCGCtgtg                          >  2:176228/1‑144 (MQ=255)
                                                                                                                  tGCAACGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCtgtg                          <  1:176252/144‑1 (MQ=255)
                                                                                                                     aaCGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑CCTGGCAAAATGGGGGCGGCCGAC‑ACCAATTAACCGCACACCCGACAGGGCGCAAAAAAAGCGGTGACCGTTGCTGCGCTGTGTGgaaaa                   >  2:508207/1‑148 (MQ=255)
                                                                                                                      aCGATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATa               <  1:656653/151‑1 (MQ=255)
                                                                                                                        gATGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAAc             <  1:785353/151‑1 (MQ=255)
                                                                                                                         aTGGGCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACg            <  1:599051/151‑1 (MQ=255)
                                                                                                                           gggCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGtt          <  2:608084/151‑1 (MQ=255)
                                                                                                                           gggCCTCGATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGt           <  2:160992/150‑1 (MQ=255)
                                                                                                                                  gATCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACCGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCCGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAAAATAACGTTGAAATgg   >  1:125667/1‑151 (MQ=255)
                                                                                                                                   aTCCGGGTCTGCGTGCTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGt  <  1:175026/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                          
GCATCTGGAAACCGAACTGATGGGCACCGTGCGCGAACGTGCGGAAGATGAAGCAATCAACGTCTTTGCCCGTAACCTGCACGATCTGCTGATGGCGGCCCCTGCCGGACTGCGTGCAACGATGGGCCTCGATCCGGGTCTGCGTACTGGGGTAAAAGTGGCGGTGGTCGATGCC‑ACTGGCAAACTGGTGGCGACCGAC‑ACCATTTACCCGCACACCGGACAGGCCGCAAAAGCAGCGATGACCGTTGCTGCGCTGTGTGAAAAACATAACGTTGAACTGGT  >  NC_000913/3538251‑3538532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A