Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,554,511 T→C L476P (CTG→CCG)  malT → mal regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,554,5110TC100.0% 208.9 / NA 59L476P (CTG→CCG) malTmal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/23);  total (36/23)

AGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTCTG  >  NC_000913/3554365‑3554655
                                                                                                                                                  |                                                                                                                                                
aGTTAACACCCTGCTATCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGTCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGACACCGGa                                                                                                                                               >  2:721448/1‑150 (MQ=255)
    aaCACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAagag                                                                                                                                           <  2:226361/150‑1 (MQ=255)
    aaCACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGc                                                                                                                                          >  1:206860/1‑151 (MQ=255)
     acacacTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCt                                                                                                                                         <  1:562412/151‑1 (MQ=255)
     acacCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCt                                                                                                                                         >  1:375355/1‑151 (MQ=255)
         ccTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgccg                                                                                                                                     <  2:14953/151‑1 (MQ=255)
           tGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgccgcc                                                                                                                                   >  2:78093/1‑151 (MQ=255)
             cTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCgg                                                                                                                                 >  2:821841/1‑151 (MQ=255)
             cTAGCCAGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCgg                                                                                                                                 >  2:519310/1‑151 (MQ=255)
                 cccGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTg                                                                                                                             >  2:311134/1‑151 (MQ=255)
                    gTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGtt                                                                                                                          >  2:370477/1‑151 (MQ=255)
                       cTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCt                                                                                                                        >  1:512845/1‑150 (MQ=255)
                         gAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCtat                                                                                                                      <  2:861381/150‑1 (MQ=255)
                             aTGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCg                                                                                                                 <  2:853262/151‑1 (MQ=255)
                               gAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGc                                                                                                                >  1:858653/1‑150 (MQ=255)
                                 aaTCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCAtt                                                                                                             >  2:735718/1‑151 (MQ=255)
                                    cAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATtgt                                                                                                           <  1:788708/150‑1 (MQ=255)
                                     aaGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTgg                                                                                                         <  1:754972/151‑1 (MQ=255)
                                       ggACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCa                                                                                                       <  2:510258/151‑1 (MQ=255)
                                               gagaAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGt                                                                                               >  2:438576/1‑151 (MQ=255)
                                                agaagaCACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCggcg                                                                                              <  2:259118/151‑3 (MQ=255)
                                                agaagaCACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTg                                                                                              <  2:375329/151‑1 (MQ=255)
                                                agaagaCACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTg                                                                                              >  2:270514/1‑151 (MQ=255)
                                                  aagaCACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCt                                                                                            >  1:514747/1‑151 (MQ=255)
                                                     acacCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGATCTATAGCCGCATTGTGGCAACCTCGGTGCTggg                                                                                         >  1:672787/1‑151 (MQ=255)
                                                         cATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGa                                                                                      >  1:178715/1‑150 (MQ=255)
                                                            gCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGtg                                                                                  >  2:790061/1‑151 (MQ=255)
                                                             cACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGtgc                                                                                 >  1:70830/1‑151 (MQ=255)
                                                              aCGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGtgct                                                                                >  1:537110/1‑151 (MQ=255)
                                                              aCGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGtgct                                                                                <  1:787257/151‑1 (MQ=255)
                                                               cGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGtgctg                                                                               >  2:178115/1‑151 (MQ=255)
                                                                 cAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGAGGCAACCTCGGTGCTGGGTGAAGtgctgc                                                                              >  1:77352/1‑150 (MQ=255)
                                                                  aGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCAGCGGAAGAGCTGCCGCCGGGATGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGctgcac                                                                            <  2:832474/151‑1 (MQ=255)
                                                                    aaTTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGctgcactg                                                                          >  2:36089/1‑151 (MQ=255)
                                                                        tAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCaa                                                                       >  2:877379/1‑150 (MQ=255)
                                                                        tAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCaa                                                                       >  1:134431/1‑150 (MQ=255)
                                                                        tAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCaa                                                                       >  2:684330/1‑150 (MQ=255)
                                                                           cGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGc                                                                   <  1:77424/151‑1 (MQ=255)
                                                                              tctGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGa                                                                 >  2:229276/1‑150 (MQ=255)
                                                                                tGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAAtt                                                              >  1:796347/1‑151 (MQ=255)
                                                                                tGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAAtt                                                              >  1:903913/1‑151 (MQ=255)
                                                                                 gcgcgcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAAtt                                                              <  1:877450/150‑1 (MQ=255)
                                                                                   gcgcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGAc                                                           >  2:113434/1‑151 (MQ=255)
                                                                                     gcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGAccc                                                         >  1:224684/1‑151 (MQ=255)
                                                                                        cAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCt                                                      <  2:888699/151‑1 (MQ=255)
                                                                                         aGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTc                                                     <  1:832113/151‑1 (MQ=255)
                                                                                           gTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCAc                                                   >  2:501227/1‑151 (MQ=255)
                                                                                                   tAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCt                                            >  2:276940/1‑150 (MQ=255)
                                                                                                           gTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATgcagc                                    >  1:600873/1‑150 (MQ=255)
                                                                                                             aaTCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCaaa                                 <  2:903840/151‑1 (MQ=255)
                                                                                                             aaTCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCaa                                  <  2:464257/150‑1 (MQ=255)
                                                                                                             aaTCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCaa                                  <  1:438623/150‑1 (MQ=255)
                                                                                                                    aTGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAAc                           >  1:172506/1‑150 (MQ=255)
                                                                                                                        aGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATg                      <  1:638011/151‑1 (MQ=255)
                                                                                                                           ggAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGc                    >  1:632897/1‑150 (MQ=255)
                                                                                                                              aCGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGc                >  1:290790/1‑151 (MQ=255)
                                                                                                                                 gCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAg             <  2:516870/151‑1 (MQ=255)
                                                                                                                                             ggCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTCTg  <  1:438838/150‑1 (MQ=255)
                                                                                                                                             ggCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGAGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTCt   <  2:514697/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTCTG  >  NC_000913/3554365‑3554655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A