Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,554,511	T→C	L476P (CTG→CCG)	malT →	mal regulon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,554,511	0	T	C	100.0%	160.9 / NA	48	L476P (CTG→CCG)	malT	mal regulon transcriptional activator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/17); total (31/17)

GAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTC  >  NC_000913/3554363‑3554653

gAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCgg                                                                                                                                              >  1:380073/1‑151 (MQ=255)

  aGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGa                                                                                                                                             >  1:558201/1‑150 (MQ=255)

           ccTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgcc                                                                                                                                    >  1:5534/1‑150 (MQ=255)

            cTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTgccgc                                                                                                                                  >  1:104311/1‑151 (MQ=255)

               cTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAAAAGACACCATGCACGCAGAATTTAACGATCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCgg                                                                                                                               >  2:322473/1‑151 (MQ=255)

                  gCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGc                                                                                                                             >  1:257260/1‑150 (MQ=255)

                           gAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCtat                                                                                                                    >  2:790905/1‑150 (MQ=255)

                           gAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCta                                                                                                                     <  1:559123/149‑1 (MQ=255)

                             aCATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGc                                                                                                                 <  1:447872/151‑1 (MQ=255)

                              cATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGcc                                                                                                                >  1:813786/1‑151 (MQ=255)

                                      cAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATtgtg                                                                                                        >  2:547118/1‑151 (MQ=255)

                                      cAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAAAGCTGCCGCCGGGCTGGTTCTATAGCCGCATtgtg                                                                                                        >  2:817078/1‑151 (MQ=255)

                                           aCATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAAc                                                                                                   >  2:596295/1‑151 (MQ=255)

                                             aTCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCt                                                                                                 <  1:592730/151‑1 (MQ=255)

                                               cAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCg                                                                                               <  1:850893/151‑1 (MQ=255)

                                                 gagaAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGt                                                                                             >  1:291010/1‑151 (MQ=255)

                                                       acacCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTggg                                                                                       >  2:366595/1‑151 (MQ=255)

                                                       acacCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTggg                                                                                       <  1:841056/151‑1 (MQ=255)

                                                          ccATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGa                                                                                    <  2:193434/151‑1 (MQ=255)

                                                           cATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGa                                                                                    >  1:438833/1‑150 (MQ=255)

                                                             tGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGt                                                                                 >  2:680803/1‑151 (MQ=255)

                                                                         ttAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGt                                                                                 <  2:373123/139‑1 (MQ=255)

                                                                         ttAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGt                                                                                 >  1:373099/1‑139 (MQ=255)

                                                                           aaCGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCaa                                                                     >  2:173636/1‑149 (MQ=255)

                                                                           aaCGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGGGCTGCACTGCAAAg                                                                   <  2:440430/151‑1 (MQ=255)

                                                                             cGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGc                                                                 >  1:825373/1‑151 (MQ=255)

                                                                             cGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGAGGCAACATCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGc                                                                 >  2:575276/1‑151 (MQ=255)

                                                                               ctctGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCg                                                                >  2:327601/1‑150 (MQ=255)

                                                                                 ctGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAAt                                                             >  1:125707/1‑151 (MQ=255)

                                                                                      cgcCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGAcc                                                        <  2:700594/151‑1 (MQ=255)

                                                                                         ccAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGc                                                     <  1:775439/151‑1 (MQ=255)

                                                                                          cAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCt                                                    >  1:121212/1‑151 (MQ=255)

                                                                                           aGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTc                                                   <  1:105858/151‑1 (MQ=255)

                                                                                             gTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCAc                                                 >  2:675118/1‑151 (MQ=255)

                                                                                                   aTTAACGCTGGTAATCCGGATGAAGCTGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGGCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAACTGACCCGCGCACTgg                                              >  1:240596/1‑148 (MQ=255)

                                                                                                      aaCGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTa                                         >  2:141690/1‑150 (MQ=255)

                                                                                                         gATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATgc                                     >  2:591227/1‑151 (MQ=255)

                                                                                                              tAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATgcagca                                 >  1:605834/1‑150 (MQ=255)

                                                                                                                  ccGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAAc                              <  1:315133/149‑1 (MQ=255)

                                                                                                                  ccGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCg                            <  2:456424/151‑1 (MQ=255)

                                                                                                                  ccGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCg                            >  2:671285/1‑151 (MQ=255)

                                                                                                                   cGGATGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCg                            >  2:470801/1‑150 (MQ=255)

                                                                                                                      aTGAAGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAAc                         <  1:795772/150‑1 (MQ=255)

                                                                                                                          aGCGGAACGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATg                    >  2:740456/1‑151 (MQ=255)

                                                                                                                                 cGGCTGGCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGcc             >  2:600381/1‑151 (MQ=255)

                                                                                                                                      ggCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCa         <  1:183313/150‑1 (MQ=255)

                                                                                                                                      ggCAAAACTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCAc        <  2:121235/151‑1 (MQ=255)

                                                                                                                                            aCTGGCACCGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTc  <  2:135227/151‑1 (MQ=255)

GAAGTTAACACCCTGCTAGCCCGTGCTGAACATGAAATCAAGGACATCAGAGAAGACACCATGCACGCAGAATTTAACGCTCTGCGCGCCCAGGTGGCGATTAACGATGGTAATCCGGATGAAGCGGAACGGCTGGCAAAACTGGCACTGGAAGAGCTGCCGCCGGGCTGGTTCTATAGCCGCATTGTGGCAACCTCGGTGCTGGGTGAAGTGCTGCACTGCAAAGGCGAATTGACCCGCTCACTGGCGCTAATGCAGCAAACCGAACAGATGGCACGCCAGCACGATGTC  >  NC_000913/3554363‑3554653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A