Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,906,128	A→G	V147A (GTG→GCG)	bglG ←	transcriptional antiterminator of the bgl operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,906,128	0	A	G	100.0%	179.7 / NA	53	V147A (GTG→GCG)	bglG	transcriptional antiterminator of the bgl operon
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/24); total (29/24)

ATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCA  >  NC_000913/3905986‑3906271

aTTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAAc                                                                                                                                         >  1:736162/1‑151 (MQ=255)

     ggCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGttt                                                                                                                                    >  1:861237/1‑151 (MQ=255)

          aactgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCa                                                                                                                                >  2:363772/1‑150 (MQ=255)

           actgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              <  1:360520/151‑1 (MQ=255)

            ctgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              >  2:207747/1‑150 (MQ=255)

            ctgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              >  2:748546/1‑150 (MQ=255)

            ctgaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaat                                                                                                                              >  1:519134/1‑150 (MQ=255)

              gaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaataat                                                                                                                           <  2:543314/151‑1 (MQ=255)

              gaaATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCaataa                                                                                                                            <  2:463920/150‑1 (MQ=255)

               aaaTTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATg                                                                                                                          <  1:258410/151‑1 (MQ=255)

                  ttttATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGtt                                                                                                                       >  2:133984/1‑151 (MQ=255)

                     tattaATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAAc                                                                                                                    >  1:563269/1‑151 (MQ=255)

                      attaATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAAc                                                                                                                    <  2:112037/150‑1 (MQ=255)

                      attaATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACg                                                                                                                   >  2:843639/1‑151 (MQ=255)

                         aaTTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGctt                                                                                                                <  2:380413/151‑1 (MQ=255)

                           ttGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGcttct                                                                                                              >  1:515439/1‑151 (MQ=255)

                             gcagcaTCTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTTACCACATGCATGGCAATAAAGCCCGCTTCATTTTTCTGTAACTGCGCGCCCCAGCGTTCATCAATAATGGTTAACActgctt                                                                                                             <  2:598932/150‑1 (MQ=255)

                                         gcATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGa                                                                                                 >  2:620737/1‑150 (MQ=255)

                                                  gCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCgg                                                                                       <  1:341612/151‑1 (MQ=255)

                                                           cTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCg                                                                              >  2:515143/1‑151 (MQ=255)

                                                             gCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGc                                                                             <  2:694555/150‑1 (MQ=255)

                                                               aaCATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTgg                                                                          >  2:716925/1‑151 (MQ=255)

                                                                aCATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGa                                                                         <  2:902650/151‑1 (MQ=255)

                                                                        cATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCcac                                                                 >  1:800486/1‑151 (MQ=255)

                                                                           atTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGc                                                              >  1:399199/1‑151 (MQ=255)

                                                                           atTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGc                                                              >  2:459577/1‑151 (MQ=255)

                                                                               ccGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAAc                                                           <  2:29781/150‑1 (MQ=255)

                                                                               ccGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACg                                                          <  2:756604/151‑1 (MQ=255)

                                                                                cGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACgg                                                         <  2:267175/151‑1 (MQ=255)

                                                                                 gCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACggg                                                        <  1:321137/151‑1 (MQ=255)

                                                                                    cATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTg                                                     >  1:588056/1‑151 (MQ=255)

                                                                                    cATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTg                                                     <  2:451935/151‑1 (MQ=255)

                                                                                        tGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGgc                                                  >  1:760454/1‑150 (MQ=255)

                                                                                           gCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGgcagc                                               >  1:742932/1‑150 (MQ=255)

                                                                                            cACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGgcagca                                              >  1:215731/1‑150 (MQ=255)

                                                                                                gACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACg                                         <  2:71314/151‑1 (MQ=255)

                                                                                                 aCCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGt                                        <  2:596026/151‑1 (MQ=255)

                                                                                                  ccAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGtt                                       <  2:774045/151‑1 (MQ=255)

                                                                                                   cAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGttt                                      <  2:61158/151‑1 (MQ=255)

                                                                                                    aGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGtttt                                     >  2:826284/1‑151 (MQ=255)

                                                                                                          aTGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCt                                  <  2:254062/148‑1 (MQ=255)

                                                                                                               aaTAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGt                          >  2:234553/1‑151 (MQ=255)

                                                                                                                 tAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTTACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGttt                        >  2:568387/1‑151 (MQ=255)

                                                                                                                 tAAAGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGttt                        >  2:890769/1‑151 (MQ=255)

                                                                                                                  aaaGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGttt                        <  1:427162/150‑1 (MQ=255)

                                                                                                                   aaGCCCACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTaa                      <  1:826351/151‑1 (MQ=255)

                                                                                                                       ccACTTCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGc                  >  2:399430/1‑151 (MQ=255)

                                                                                                                            tCATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACt             >  2:831852/1‑151 (MQ=255)

                                                                                                                             cATCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACt             <  2:588007/150‑1 (MQ=255)

                                                                                                                               tCTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGc          >  1:647275/1‑151 (MQ=255)

                                                                                                                                cTTTCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGc          >  2:289429/1‑150 (MQ=255)

                                                                                                                                   tCGGTAACTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATg      >  2:602317/1‑151 (MQ=255)

                                                                                                                                        aaCTGCGCGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCa  <  1:478949/150‑1 (MQ=255)

ATTAAGGCTGAACTGAAATTTTATTAATTGCAGCATTTCGCGCATTAACTGCGTGACACCTGCAACATCCTCCATATTTCCGCTCATTTGGGCACTGACCAGATGCATGGCAATAAAGCCCACTTCATCTTTCGGTAACTGCACGCCCAACCGTTTATCAATAATGGTTAACGCTTCTTCCCCTAGCTGGAACTCTTTCGGGTAAAGCCGCTGGATATCCCACAGCAACGGGTTGGGCAGCAACACGTTTTGCTGAAAGCGTTTAATCGCAAACTGGCAATGGTCA  >  NC_000913/3905986‑3906271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A