Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I1 R1
|
300 |
54.7 |
1718999 |
98.4% |
1691495 |
149.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,913,871 |
T→C |
T444A (ACT→GCT) |
glmU ← |
fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase |
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,913,871 | 0 | T | C | 100.0%
| 207.3
/ NA
| 59 | T444A (ACT→GCT) | glmU | fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/31); total (28/31) |
GGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGT > NC_000913/3913723‑3914018
|
gggCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCt < 1:389976/151‑1 (MQ=255)
gcCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCgg > 1:144013/1‑151 (MQ=255)
gAGGCTTGTAGAGAGGGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTAACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCacac > 1:605705/1‑151 (MQ=255)
aGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACg > 1:774981/1‑151 (MQ=255)
gCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACgg < 2:110377/151‑1 (MQ=255)
ccTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGc < 2:577063/151‑1 (MQ=255)
cTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCt > 2:279743/1‑151 (MQ=255)
tGTAGAGAGCGGGGTTATTTTTATAGTTACTGCTTGCGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTg > 1:415659/1‑150 (MQ=255)
agagTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCt < 1:370343/151‑1 (MQ=255)
agagTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCt < 1:518948/151‑1 (MQ=255)
gagTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCt > 2:839955/1‑150 (MQ=255)
gagTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTa < 2:357596/151‑1 (MQ=255)
tGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATg > 1:611792/1‑151 (MQ=255)
ggggTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGc < 1:632605/151‑1 (MQ=255)
ggTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCAt > 1:689910/1‑151 (MQ=255)
ggTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAAAGCAt > 2:804196/1‑151 (MQ=255)
gTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCAtt > 2:37886/1‑151 (MQ=255)
gTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCAtt < 1:694674/151‑1 (MQ=255)
tttttATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTc < 1:335072/150‑1 (MQ=255)
ttATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCg > 2:715645/1‑151 (MQ=255)
ataGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAc < 1:514654/151‑1 (MQ=255)
ataGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAc < 1:850174/151‑1 (MQ=255)
ataGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAc > 1:808724/1‑151 (MQ=255)
ataGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAc < 1:622449/151‑1 (MQ=255)
ataGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGAc > 1:486185/1‑151 (MQ=255)
aCTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTCCGCCGACATTAc < 2:573138/150‑1 (MQ=255)
tgtgGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTc < 2:588585/149‑1 (MQ=255)
tgtgGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTc < 2:144036/149‑1 (MQ=255)
tgGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTcac > 1:651536/1‑149 (MQ=255)
gggagggaGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAg < 2:87667/150‑1 (MQ=255)
ggagggagATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGtt > 1:469459/1‑151 (MQ=255)
gggagATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTa < 2:405777/151‑1 (MQ=255)
gagaTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTAcc > 1:793344/1‑151 (MQ=255)
aTTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCgc > 1:497219/1‑151 (MQ=255)
aTGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCgcagc < 1:360290/151‑1 (MQ=255)
aTGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCgcagc > 2:332571/1‑151 (MQ=255)
ttATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAAt > 1:493281/1‑151 (MQ=255)
tATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATg > 1:242232/1‑151 (MQ=255)
cctcATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTcg < 2:808780/150‑1 (MQ=255)
cccATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCttt < 2:403021/151‑1 (MQ=255)
cccATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCttt > 2:584987/1‑151 (MQ=255)
gtgACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCt < 2:675881/150‑1 (MQ=255)
aCCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTg > 2:93197/1‑151 (MQ=255)
ccGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGt > 2:304255/1‑151 (MQ=255)
cGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGtt < 2:611832/151‑1 (MQ=255)
gggTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTa < 1:181321/151‑1 (MQ=255)
cGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGcc > 2:499309/1‑151 (MQ=255)
cAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGccacca < 2:829465/151‑1 (MQ=255)
aGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAg < 1:472797/151‑1 (MQ=255)
ttCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCg < 2:487611/150‑1 (MQ=255)
tCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCgg > 2:518457/1‑150 (MQ=255)
cGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACcaaca < 2:668134/151‑1 (MQ=255)
acgacgCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCaacaaac < 1:406885/151‑1 (MQ=255)
gCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtc < 2:51597/151‑1 (MQ=255)
gCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtc > 1:585663/1‑151 (MQ=255)
ccTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGc < 2:806240/151‑1 (MQ=255)
tctttctGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGcc < 1:770897/149‑1 (MQ=255)
tctttctGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCg > 2:588245/1‑150 (MQ=255)
gAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGt > 1:153049/1‑151 (MQ=255)
|
GGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGT > NC_000913/3913723‑3914018
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A