Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,913,871	T→C	T444A (ACT→GCT)	glmU ←	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,913,871	0	T	C	100.0%	146.9 / NA	41	T444A (ACT→GCT)	glmU	fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/26); total (15/26)

GGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGT  >  NC_000913/3913723‑3914018

gggCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGCGATTATGTTATCTACTCATCCCATGTGACCGGGTTAGCCGGCCAGCATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCt                                                                                                                                                   >  1:457824/1‑151 (MQ=255)

 ggCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTg                                                                                                                                                  <  2:759826/151‑1 (MQ=255)

   cgcCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCg                                                                                                                                                <  2:723029/151‑1 (MQ=255)

         gAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCacac                                                                                                                                          <  2:144142/151‑1 (MQ=255)

               tGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTg                                                                                                                                     <  2:287312/150‑1 (MQ=255)

               tGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTg                                                                                                                                     <  1:124145/150‑1 (MQ=255)

                gTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGAt                                                                                                                                   >  1:353772/1‑151 (MQ=255)

                      agTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTaa                                                                                                                             <  2:535002/151‑1 (MQ=255)

                             ttATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCAttt                                                                                                                      >  1:636009/1‑151 (MQ=255)

                               aTTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCAtttt                                                                                                                     <  2:746752/150‑1 (MQ=255)

                               aTTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTc                                                                                                                    >  2:678794/1‑151 (MQ=255)

                               aTTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTc                                                                                                                    <  1:621913/151‑1 (MQ=255)

                                       aGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACa                                                                                                             <  1:446513/150‑1 (MQ=255)

                                       aGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAt                                                                                                            <  2:78860/151‑1 (MQ=255)

                                       aGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAACGCATTTTCGCCGACAt                                                                                                            <  1:677833/151‑1 (MQ=255)

                                        gTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAtt                                                                                                           <  1:500930/151‑1 (MQ=255)

                                        gTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAtt                                                                                                           <  2:434359/151‑1 (MQ=255)

                                         ttACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACAtt                                                                                                           >  2:186898/1‑150 (MQ=255)

                                          tACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTa                                                                                                          <  2:262896/150‑1 (MQ=255)

                                            cTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTAcg                                                                                                        <  1:759471/150‑1 (MQ=255)

                                              gCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGTACACGGCTGATAGCTAATGCATTTTCGCCGACATTAcgc                                                                                                       <  2:772509/149‑1 (MQ=255)

                                               cTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTc                                                                                                    >  2:575744/1‑151 (MQ=255)

                                                               tATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCCGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCg                                                                                       >  1:561098/1‑148 (MQ=255)

                                                                 tGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCgcagc                                                                                   >  1:742104/1‑150 (MQ=255)

                                                                        tcctcATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTcg                                                                           >  1:540567/1‑151 (MQ=255)

                                                                            cATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGcc                                                                       >  1:731874/1‑151 (MQ=255)

                                                                                ccATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTg                                                                   <  2:396922/151‑1 (MQ=255)

                                                                                     tgACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTAc                                                              >  2:587061/1‑151 (MQ=255)

                                                                                            gTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTAcc                                                       <  2:206763/151‑1 (MQ=255)

                                                                                                  cGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGcc                                                 >  2:52983/1‑151 (MQ=255)

                                                                                                         aaTCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCt                                          <  1:333668/151‑1 (MQ=255)

                                                                                                         aaTCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCt                                          <  1:302833/151‑1 (MQ=255)

                                                                                                           tCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTg                                         >  2:550219/1‑150 (MQ=255)

                                                                                                            cACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGa                                        <  1:278094/150‑1 (MQ=255)

                                                                                                                ttttCTTTACCGGACGACGCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTc                                   >  1:759520/1‑151 (MQ=255)

                                                                                                                                acgCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAAcaca                   <  2:561618/151‑1 (MQ=255)

                                                                                                                                  gCCAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtc                 >  1:23604/1‑151 (MQ=255)

                                                                                                                                    cAGCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtcgt               <  2:636066/151‑1 (MQ=255)

                                                                                                                                      gCCTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACAtcgtcg             <  2:687722/151‑1 (MQ=255)

                                                                                                                                       ccTTCTTTCTGAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGc            <  2:42386/151‑1 (MQ=255)

                                                                                                                                                 gAGCCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGt  <  2:291071/151‑1 (MQ=255)

GGGCGCCCCGAGCCTTGTAGAGAGTGGGGTTATTTTTATAGTTACTGCTTGTGGGAGGGAGATTATGTTATCTCCTCATCCCATGTGACCGGGTTAGCCGGCCAGAATCACTTTTTCTTTACCGGACGACGCCAGCCTTCTTTCTGAGTCTGCGGCACACGGCTGATAGCTAATGCATTTTCGCCGACATTACGCGTCACAGTTGTACCCGCAGCAATGGTCGCGCCTTTGCCTACTGTTACCGGGGCCACCAGCTGAGTGTCGGAACCAACAAACACATCGTCGCCGATAATGGT  >  NC_000913/3913723‑3914018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A