Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 283 58.3 1819865 98.8% 1798026 150.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,930,513 T→C Q194R (CAG→CGG)  ravA ← hexameric AAA+ MoxR family ATPase, putative molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,5130TC100.0% 178.0 / NA 49Q194R (CAG→CGG) ravAhexameric AAA+ MoxR family ATPase, putative molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/22);  total (27/22)

GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATC  >  NC_000913/3930366‑3930656
                                                                                                                                                   |                                                                                                                                               
ggTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGtt                                                                                                                                              <  2:212773/151‑1 (MQ=255)
 gTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTg                                                                                                                                             >  1:130110/1‑151 (MQ=255)
   aaTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGa                                                                                                                                            <  1:482209/150‑1 (MQ=255)
   aaTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGAc                                                                                                                                           >  1:764637/1‑151 (MQ=255)
    aTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACt                                                                                                                                          <  2:756447/151‑1 (MQ=255)
        aTCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGt                                                                                                                                       <  2:391694/150‑1 (MQ=255)
          ccAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCa                                                                                                                                     <  1:574678/150‑1 (MQ=255)
                gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                              <  2:466935/151‑1 (MQ=255)
                gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                              <  2:194031/151‑1 (MQ=255)
                  tcgcgcAGCATAAAAATCAGCTCAAATACATGATCGGCCAGTGTAATTTCACCCATCTCTTTCTGCCAGTGTTCATATTCTACATCTGTGACCTGCAAGGCATCAGGAACGGGTTTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                              >  2:10396/3‑149 (MQ=255)
                    gcgcAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCgg                                                                                                                          <  1:9806/151‑1 (MQ=255)
                          caTAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTc                                                                                                                    >  2:716565/1‑151 (MQ=255)
                                 aTCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTAt                                                                                                             >  2:372563/1‑151 (MQ=255)
                                        cAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCAc                                                                                                      >  2:703320/1‑151 (MQ=255)
                                         aaaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACt                                                                                                     >  1:37452/1‑151 (MQ=255)
                                         aaaTACATGATCGGGCAGCGAAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCAGTGACCTGCAAGGCATCAGAAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACt                                                                                                     >  2:43492/1‑151 (MQ=255)
                                          aaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACtt                                                                                                    <  2:38417/151‑1 (MQ=255)
                                           aTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACttt                                                                                                   >  2:484215/1‑151 (MQ=255)
                                             aCATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTAt                                                                                                 >  2:147851/1‑151 (MQ=255)
                                              cATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATc                                                                                                >  1:314013/1‑151 (MQ=255)
                                               aTGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCt                                                                                               <  1:858537/151‑1 (MQ=255)
                                                 gATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCAACTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCAACCCGTTGACTGATCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTAATCTa                                                                                              <  2:858603/150‑1 (MQ=255)
                                                  aTCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAc                                                                                            >  1:279058/1‑151 (MQ=255)
                                                  aTCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAc                                                                                            >  1:720279/1‑151 (MQ=255)
                                                         aGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGAc                                                                                     <  1:281714/151‑1 (MQ=255)
                                                                   cACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCAt                                                                           >  1:50809/1‑151 (MQ=255)
                                                                    aCCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATg                                                                          >  2:667689/1‑151 (MQ=255)
                                                                      cAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCg                                                                        <  1:628742/151‑1 (MQ=255)
                                                                        aTCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGt                                                                      >  1:862452/1‑151 (MQ=255)
                                                                         tctcTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTc                                                                     >  1:633720/1‑151 (MQ=255)
                                                                              ttCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCata                                                                  <  2:409672/149‑1 (MQ=255)
                                                                                cTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAAc                                                              >  2:876462/1‑151 (MQ=255)
                                                                                 tGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACg                                                             >  1:229634/1‑151 (MQ=255)
                                                                                  gCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAAc                                                              <  1:753135/149‑1 (MQ=255)
                                                                                          tCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGAc                                                    >  2:209977/1‑151 (MQ=255)
                                                                                                tcttcATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGActgctg                                               <  2:879468/150‑1 (MQ=255)
                                                                                                tcttcATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGActgctg                                               <  1:524915/150‑1 (MQ=255)
                                                                                                    cATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGc                                          <  1:103112/151‑1 (MQ=255)
                                                                                                     aTCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCATAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCt                                         >  1:9667/1‑151 (MQ=255)
                                                                                                      tCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCtt                                        >  2:635917/1‑151 (MQ=255)
                                                                                                                gCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCt                               <  2:857316/150‑1 (MQ=255)
                                                                                                                     gCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTgg                         <  1:876533/151‑1 (MQ=255)
                                                                                                                         cAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgc                     >  2:722084/1‑151 (MQ=255)
                                                                                                                             aaCCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgccgcc                 >  1:70949/1‑151 (MQ=255)
                                                                                                                               ccGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGccac               >  2:88128/1‑151 (MQ=255)
                                                                                                                                 ggATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGccacc              >  1:828966/1‑150 (MQ=255)
                                                                                                                                      gTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACcagcag         <  1:815133/150‑1 (MQ=255)
                                                                                                                                        cGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGAc       <  2:512754/150‑1 (MQ=255)
                                                                                                                                            ttCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATc  >  1:615901/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATC  >  NC_000913/3930366‑3930656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A