Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,930,513 T→C Q194R (CAG→CGG)  ravA ← hexameric AAA+ MoxR family ATPase, putative molecular chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,5130TC100.0% 151.5 / NA 42Q194R (CAG→CGG) ravAhexameric AAA+ MoxR family ATPase, putative molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/20);  total (22/20)

GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCGGG  >  NC_000913/3930366‑3930659
                                                                                                                                                   |                                                                                                                                                  
ggTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGtt                                                                                                                                                 >  2:102697/1‑151 (MQ=255)
   aaTTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGAc                                                                                                                                              >  2:98650/1‑151 (MQ=255)
       tATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTgg                                                                                                                                           <  2:585830/150‑1 (MQ=255)
        aTCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACAAGATCGGGCAGCGTAATTTCCCCAATCGCATTCGGCCGGCGTTCATATTCTTCATCTGTGCCCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGt                                                                                                                                          <  1:121925/150‑1 (MQ=255)
            aGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCa                                                                                                                                     <  2:659412/151‑1 (MQ=255)
                gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATgg                                                                                                                                  >  1:27672/1‑150 (MQ=255)
                gCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGa                                                                                                                                 <  2:701680/151‑1 (MQ=255)
                   ggCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAg                                                                                                                                <  2:145735/149‑1 (MQ=255)
                          caTAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTc                                                                                                                       >  1:483442/1‑151 (MQ=255)
                           aTAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCg                                                                                                                      >  2:615068/1‑151 (MQ=255)
                             aaaaaTCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCt                                                                                                                    <  1:92809/151‑1 (MQ=255)
                               aaaTCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCttt                                                                                                                  <  2:22845/151‑1 (MQ=255)
                                  tCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATc                                                                                                               >  2:680757/1‑151 (MQ=255)
                                          aaTACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACtt                                                                                                       >  2:461769/1‑151 (MQ=255)
                                             aCATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTa                                                                                                     <  1:546427/150‑1 (MQ=255)
                                                 gATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTaa                                                                                                <  1:372050/151‑1 (MQ=255)
                                                 gATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTa                                                                                                 <  1:44051/150‑1 (MQ=255)
                                                   tCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAAcc                                                                                              >  1:292160/1‑151 (MQ=255)
                                                       gCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACcac                                                                                            >  2:13676/1‑149 (MQ=255)
                                                         aGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGAc                                                                                        <  2:26838/151‑1 (MQ=255)
                                                              aaTTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAAt                                                                                    <  2:735575/150‑1 (MQ=255)
                                                              aaTTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATc                                                                                   >  1:29657/1‑151 (MQ=255)
                                                                   cACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCAt                                                                              <  1:199703/151‑1 (MQ=255)
                                                                                 tGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACg                                                                >  1:538951/1‑151 (MQ=255)
                                                                                  gCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATaa                                                                  <  2:417190/148‑1 (MQ=255)
                                                                                   ccAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCt                                                              <  1:484706/151‑1 (MQ=255)
                                                                                    cAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTa                                                                                                 >  2:236266/1‑115 (MQ=255)
                                                                                    cAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTa                                                                                                 <  1:236233/115‑1 (MQ=255)
                                                                                    cAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCtt                                                             >  2:675452/1‑151 (MQ=255)
                                                                                            atatTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGActg                                                     >  1:412425/1‑151 (MQ=255)
                                                                                                    cATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTg                                              <  1:173623/150‑1 (MQ=255)
                                                                                                      tCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCtt                                           >  2:643075/1‑151 (MQ=255)
                                                                                                         gtgACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCg                                        <  2:75150/151‑1 (MQ=255)
                                                                                                           gACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGc                                      >  1:14344/1‑151 (MQ=255)
                                                                                                           gACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGc                                      <  2:137497/151‑1 (MQ=255)
                                                                                                            aCCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCa                                     <  1:39746/151‑1 (MQ=255)
                                                                                                                gCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTc                                 >  2:522001/1‑151 (MQ=255)
                                                                                                                 cAAGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCg                                >  1:222168/1‑151 (MQ=255)
                                                                                                                  aaGGCATCAGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGt                               >  2:504395/1‑151 (MQ=255)
                                                                                                                          aGGAACCGGATTGTCGTTTTCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGgcc                       >  1:551516/1‑151 (MQ=255)
                                                                                                                                            ttCATCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATc     >  2:169686/1‑151 (MQ=255)
                                                                                                                                               aTCCCGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCTACCAGCAGACGCATCggg  >  1:407217/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTAATTTATCCAGTTGCTGGCGCAGCATAAAAATCAGCTCAAATACATGATCGGGCAGCGTAATTTCACCAATCTCTTTCTGCCAGCGTTCATATTCTTCATCTGTGACCTGCAAGGCATCAGGAACCGGATTGTCGTTTTCATCCTGTTGACTGGTCAGCATGGAGCGGAAATTCGCTTTATCCTGCACTTTATCTAACCACAGACGAATCAGCATGCGGTCATATAACGCTTCCAGACTGCTGTCTGCTTCCGGCAGCTCGTTGGAGGCCGCCACCAGCAGACGCATCGGG  >  NC_000913/3930366‑3930659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A