Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I3 R1	283	58.3	1819865	98.8%	1798026	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,246	T→C	V277A (GTT→GCT)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,246	0	T	C	100.0%	184.4 / NA	51	V277A (GTT→GCT)	rho	transcription termination factor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/26); total (25/26)

GCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTA  >  NC_000913/3967101‑3967392

gCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTc                                                                                                                                               <  1:875057/151‑1 (MQ=255)

gCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTc                                                                                                                                               >  1:575701/1‑151 (MQ=255)

  ttCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCg                                                                                                                                             >  2:171533/1‑151 (MQ=255)

   tCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCgg                                                                                                                                            >  1:149494/1‑151 (MQ=255)

        cTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGGTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTACGGCg                                                                                                                                          >  2:786125/1‑148 (MQ=255)

           tGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTACGGCGTCAGGt                                                                                                                                    >  2:718641/1‑151 (MQ=255)

             aCGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTa                                                                                                                                   >  1:888224/1‑150 (MQ=255)

                aaCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAgt                                                                                                                               <  1:693684/151‑1 (MQ=255)

                 aCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAgtg                                                                                                                              <  2:577089/151‑1 (MQ=255)

                 aCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAgtg                                                                                                                              <  2:516224/151‑1 (MQ=255)

                          tcGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggt                                                                                                                     >  1:243457/1‑151 (MQ=255)

                           cGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggtg                                                                                                                    <  2:888153/151‑1 (MQ=255)

                           cGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggtg                                                                                                                    <  2:276106/151‑1 (MQ=255)

                                    cAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATg                                                                                                           <  2:183774/151‑1 (MQ=255)

                                            ggAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGcc                                                                                                   <  2:863610/151‑1 (MQ=255)

                                              aaaTGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCt                                                                                                 <  1:90262/151‑1 (MQ=255)

                                               aaTGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTg                                                                                                >  1:194713/1‑151 (MQ=255)

                                                     gATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACGCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCg                                                                                           <  2:787047/150‑1 (MQ=255)

                                                      aTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTc                                                                                         >  2:479193/1‑151 (MQ=255)

                                                        cGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCg                                                                                       >  2:297272/1‑151 (MQ=255)

                                                        cGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCg                                                                                       <  1:871334/151‑1 (MQ=255)

                                                          agaAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGa                                                                                      >  1:415149/1‑150 (MQ=255)

                                                           gaAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGaaa                                                                                    <  2:76574/151‑1 (MQ=255)

                                                              ggCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGc                                                                                 >  1:594676/1‑151 (MQ=255)

                                                               gCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGct                                                                                >  1:164670/1‑151 (MQ=255)

                                                                      gCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTgg                                                                         >  2:907765/1‑151 (MQ=255)

                                                                        cTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTg                                                                       >  1:846631/1‑151 (MQ=255)

                                                                          ggTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTg                                                                       <  1:627263/149‑1 (MQ=255)

                                                                                   caAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAAc                                                            >  1:526818/1‑151 (MQ=255)

                                                                                            cGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAag                                                     >  2:426784/1‑149 (MQ=255)

                                                                                                   aTTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCa                                            <  1:56289/151‑1 (MQ=255)

                                                                                                      cTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGcc                                         >  1:854851/1‑151 (MQ=255)

                                                                                                           cGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGAcc                                    >  2:44023/1‑151 (MQ=255)

                                                                                                                 cATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATc                              >  1:288937/1‑151 (MQ=255)

                                                                                                                        cGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACgg                       <  2:409471/151‑1 (MQ=255)

                                                                                                                         gTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGgc                      <  1:656550/151‑1 (MQ=255)

                                                                                                                         gTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGgc                      <  1:329849/151‑1 (MQ=255)

                                                                                                                         gTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGgc                      <  1:789621/151‑1 (MQ=255)

                                                                                                                             ggCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGgcg                     <  2:594627/148‑1 (MQ=255)

                                                                                                                             ggCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCt                   <  2:367537/150‑1 (MQ=255)

                                                                                                                             ggCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCt                   <  2:215822/150‑1 (MQ=255)

                                                                                                                               cgcgcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTAt                <  2:323959/151‑1 (MQ=255)

                                                                                                                                gcgcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATc               >  2:856240/1‑151 (MQ=255)

                                                                                                                                    gcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATa           <  2:72151/151‑1 (MQ=255)

                                                                                                                                       tACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATAc          <  1:556036/149‑1 (MQ=255)

                                                                                                                                         caacaCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGt      >  2:685582/1‑151 (MQ=255)

                                                                                                                                         caacaCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGt      >  1:752844/1‑151 (MQ=255)

                                                                                                                                         caacaCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGt      <  2:142985/151‑1 (MQ=255)

                                                                                                                                          aacaCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGtt     <  1:713921/151‑1 (MQ=255)

                                                                                                                                          aacaCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGtt     >  1:748962/1‑151 (MQ=255)

                                                                                                                                              ccGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGTGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTa  >  1:207573/1‑150 (MQ=255)

GCTTCTACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTA  >  NC_000913/3967101‑3967392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A