Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I1 R1 300 54.7 1718999 98.4% 1691495 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,967,714 C→T intergenic (+38/‑202) rho → / → wecA transcription termination factor/UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,967,7140CT100.0% 206.5 / NA 56intergenic (+38/‑202)rho/wecAtranscription termination factor/UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/37);  total (19/37)

CGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTG  >  NC_000913/3967566‑3967859
                                                                                                                                                    |                                                                                                                                                 
cgcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGgtgt                                                                                                                                                 <  1:716239/151‑1 (MQ=255)
 gcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGtt                                                                                                                                                <  1:607400/151‑1 (MQ=255)
 gcAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGtt                                                                                                                                                <  1:464251/151‑1 (MQ=255)
      aTCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCt                                                                                                                                           <  2:321722/151‑1 (MQ=255)
           tCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTta                                                                                                                                       <  2:441588/150‑1 (MQ=255)
           tCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTta                                                                                                                                       <  1:20073/150‑1 (MQ=255)
            cACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTtata                                                                                                                                     <  2:676921/151‑1 (MQ=255)
             aCCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTatat                                                                                                                                    <  1:31068/151‑1 (MQ=255)
                 gATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGt                                                                                                                                >  2:454056/1‑151 (MQ=255)
                  aTGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGt                                                                                                                                >  1:176053/1‑150 (MQ=255)
                  aTGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTa                                                                                                                               <  1:498161/151‑1 (MQ=255)
                       cGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCtt                                                                                                                          <  2:779542/151‑1 (MQ=255)
                         aaaTCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTa                                                                                                                         >  1:223699/1‑150 (MQ=255)
                           aTCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATg                                                                                                                      <  2:12883/151‑1 (MQ=255)
                           aTCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATg                                                                                                                      >  2:281313/1‑151 (MQ=255)
                                      ggAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGc                                                                                                            <  1:593054/150‑1 (MQ=255)
                                      ggAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCg                                                                                                           <  1:318996/151‑1 (MQ=255)
                                           tCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGtt                                                                                                      >  2:741759/1‑151 (MQ=255)
                                            ccTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTa                                                                                                     <  2:724044/151‑1 (MQ=255)
                                             cTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGtt                                                                                                      >  1:594431/1‑149 (MQ=255)
                                                aTTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGaa                                                                                                 <  1:824895/151‑1 (MQ=255)
                                                   aataaACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAAt                                                                                              <  2:636366/151‑1 (MQ=255)
                                                      aaaCTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTcc                                                                                           >  1:261139/1‑151 (MQ=255)
                                                       aaCTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCt                                                                                          <  1:855564/151‑1 (MQ=255)
                                                        aCTTGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTg                                                                                         <  1:55901/151‑1 (MQ=255)
                                                           ggCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGa                                                                                       <  1:125845/150‑1 (MQ=255)
                                                              aaTGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAAttt                                                                                   <  2:757084/151‑1 (MQ=255)
                                                               aTGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTg                                                                                  <  1:179995/151‑1 (MQ=255)
                                                                 gaccaagaccaaTGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCt                                                                                >  2:162895/1‑151 (MQ=255)
                                                                 gaccaagaccaaTGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCt                                                                                <  1:59254/151‑1 (MQ=255)
                                                                   ccaagaccaaTGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTgg                                                                              >  2:796008/1‑151 (MQ=255)
                                                                           aaTGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTAt                                                                      <  2:265628/151‑1 (MQ=255)
                                                                                 gATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAGTCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAAtt                                                                <  1:498300/151‑1 (MQ=255)
                                                                                     tcttcGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGc                                                             >  1:12745/1‑150 (MQ=255)
                                                                                     tcttcGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGc                                                             >  2:676709/1‑150 (MQ=255)
                                                                                        tcGAAATGATGAAACGCTCATAGATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCa                                                            <  2:561145/148‑1 (MQ=255)
                                                                                        tcGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCatat                                                         >  2:560608/1‑151 (MQ=255)
                                                                                        tcGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCata                                                          <  1:553291/150‑1 (MQ=255)
                                                                                        tcGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCa                                                            <  1:551899/148‑1 (MQ=255)
                                                                                          gAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATc                                                        >  1:615008/1‑150 (MQ=255)
                                                                                            aaTGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCaaa                                                     <  1:577323/151‑1 (MQ=255)
                                                                                                       gCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAAtttt                                          <  2:434340/151‑1 (MQ=255)
                                                                                                           aTAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGc                                       >  2:205550/1‑150 (MQ=255)
                                                                                                             aaaTTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGcaca                                    <  1:361062/151‑1 (MQ=255)
                                                                                                              aaTTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCaa                                                                  >  1:50357/1‑120 (MQ=255)
                                                                                                              aaTTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCaa                                                                  <  2:50379/120‑1 (MQ=255)
                                                                                                                 ttGTCTTATGCCAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGa                                 <  1:429042/150‑1 (MQ=255)
                                                                                                                   gTCTTATGCCAACAACGCCACGTGTGTACGGGGTGCTTTGCTTTTATATCTGTAATCTTAATGCCTCGCTGGCGATGTTACGAAAATTCCTGGAATTTGCTGGCATGTTATGCCAGTTTCATGTCAAATGGTTAATTTTTGCACAGGagt                               <  1:46072/150‑3 (MQ=255)
                                                                                                                            cAAAAACGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGCGGGttt                      >  1:664175/1‑150 (MQ=255)
                                                                                                                             aaaaaCGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTgg                    <  1:290938/151‑1 (MQ=255)
                                                                                                                              aaaaCGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTgg                    >  1:15852/1‑150 (MQ=255)
                                                                                                                               aaaCGCCACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGaa                  <  2:662059/151‑1 (MQ=255)
                                                                                                                                     cACGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACt            >  1:363492/1‑151 (MQ=255)
                                                                                                                                       cGTGTTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCGAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACttt          >  2:375859/1‑151 (MQ=255)
                                                                                                                                        gtgtTTACGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTc         <  1:647700/151‑1 (MQ=255)
                                                                                                                                              aCGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGGGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTc    <  1:480424/150‑1 (MQ=255)
                                                                                                                                               cGTGGTGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTg  <  1:426827/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGCAAAATCATTCACCCGATGGGCGAAATCGATGCAATGGAATTCCTCATTAATAAACTGGCAATGACCAAGACCAATGACGATTTCTTCGAAATGATGAAACGCTCATAAATTTGTCTTATGCCAAAAACGCCACGTGTTTACGTGGCGTTTTGCTTTTATATCTGTAATCTTAATGCCGCGCTGGCGATGTTAGGAAAATTCCTGGAATTTGCTGGCATGTTATGCAATTTGCATATCAAATGGTTAATTTTTGCACAGGACTGGTGGGTTTGGAACGGACTTTCCCTTCTG  >  NC_000913/3967566‑3967859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A