Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,246	T→C	V277A (GTT→GCT)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,246	0	T	C	100.0%	170.5 / NA	46	V277A (GTT→GCT)	rho	transcription termination factor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/25); total (21/25)

ACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGT  >  NC_000913/3967107‑3967388

aCCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCg                                                                                                                                      >  1:92519/1‑150 (MQ=255)

  cTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTc                                                                                                                                    >  1:182095/1‑150 (MQ=255)

     tGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGt                                                                                                                                >  1:39854/1‑151 (MQ=255)

      gACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGt                                                                                                                                >  1:211358/1‑150 (MQ=255)

           aCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAgtg                                                                                                                          <  1:369400/151‑1 (MQ=255)

           aCCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCACTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAgtg                                                                                                                          >  2:668167/1‑151 (MQ=255)

             ccGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGtt                                                                                                                        <  2:150128/151‑1 (MQ=255)

                    tcGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggt                                                                                                                 >  1:211901/1‑151 (MQ=255)

                     cGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGAcc                                                                                                                    <  1:775358/147‑1 (MQ=255)

                     cGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggtg                                                                                                                >  2:593925/1‑151 (MQ=255)

                       ccACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCggtggt                                                                                                              <  2:595951/151‑1 (MQ=255)

                              cAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGAt                                                                                                        >  2:165286/1‑150 (MQ=255)

                               aGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGc                                                                                                      <  2:601546/151‑1 (MQ=255)

                                 gTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCa                                                                                                    <  1:69816/151‑1 (MQ=255)

                                 gTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGCGGTGTGGATGcc                                                                                                     >  1:696556/1‑150 (MQ=255)

                                          aTGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGc                                                                                           >  2:581088/1‑151 (MQ=255)

                                            ggTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCAt                                                                                         <  2:445473/151‑1 (MQ=255)

                                             gTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATc                                                                                        <  2:518132/151‑1 (MQ=255)

                                               gATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCg                                                                                       <  1:252903/150‑1 (MQ=255)

                                                aTCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTc                                                                                     <  2:745993/151‑1 (MQ=255)

                                                 tCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTcc                                                                                    >  2:556329/1‑151 (MQ=255)

                                                               cGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTg                                                                      >  2:49291/1‑151 (MQ=255)

                                                               cGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTg                                                                      >  1:94980/1‑151 (MQ=255)

                                                                        gAGCGCAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGTCCGGTGGTGTGGATGCCTACGCCCTGAATCGTCCGAAACGCTTCTTTAGTgcggcg                                                              <  1:330108/150‑1 (MQ=255)

                                                                         aGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGcgcg                                                            <  2:506933/151‑1 (MQ=255)

                                                                          gCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGt                                                           <  2:480755/151‑1 (MQ=255)

                                                                           cacaAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTa                                                          <  2:422191/151‑1 (MQ=255)

                                                                             caAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAAc                                                        >  1:45406/1‑151 (MQ=255)

                                                                               agaaagaCGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGt                                                      <  1:713690/151‑1 (MQ=255)

                                                                                         tATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAggg                                             <  1:548842/150‑1 (MQ=255)

                                                                                          aTCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGggcg                                           <  1:183622/151‑1 (MQ=255)

                                                                                              ttCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGggcgg                                          >  1:392874/1‑148 (MQ=255)

                                                                                                   cTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTg                                   >  2:597538/1‑150 (MQ=255)

                                                                                                     cGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGAcc                                >  1:24864/1‑151 (MQ=255)

                                                                                                         tCCATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCAtt                             >  1:671505/1‑150 (MQ=255)

                                                                                                          ccATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTa                            <  2:329258/150‑1 (MQ=255)

                                                                                                          ccATCACTCGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTAt                           <  1:540491/151‑1 (MQ=255)

                                                                                                                  cGTCTGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACg                    <  2:84388/150‑1 (MQ=255)

                                                                                                                      tGGCGCGCGCTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGgcgc                >  1:746353/1‑150 (MQ=255)

                                                                                                                         cgcgcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTAt            <  2:17461/151‑1 (MQ=255)

                                                                                                                          gcgcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATc           <  2:37763/151‑1 (MQ=255)

                                                                                                                            gcgcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGa         <  1:668109/151‑1 (MQ=255)

                                                                                                                              gcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCGGCCTGACCATTATCGCGACGGCGCTTATCGATa       <  1:91651/151‑1 (MQ=255)

                                                                                                                              gcTTACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATa       <  2:397597/151‑1 (MQ=255)

                                                                                                                                ttACAACACCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATAcc     >  1:147350/1‑151 (MQ=255)

                                                                                                                                   caacaCCGCTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGt  >  2:403709/1‑151 (MQ=255)

ACCTTTGACGAACCCGCATCTCGCCACGTTCAGGTTGCGGAAATGGTGATCGAGAAGGCCAAACGCCTGGTTGAGCACAAGAAAGACGTTATCATTCTGCTCGACTCCATCACTCGTCTGGCGCGCGCTTACAACACCGTTGTTCCGGCGTCAGGTAAAGTGTTGACCGGTGGTGTGGATGCCAACGCCCTGCATCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGT  >  NC_000913/3967107‑3967388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A