Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I3 R1
|
283 |
58.3 |
1819865 |
98.8% |
1798026 |
150.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,556,769 |
(T)9→10 |
intergenic (‑449/‑224) |
yjiC ← / → iraD |
uncharacterized protein/RpoS stabilzer after DNA damage, anti‑RssB factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,556,760 | 1 | . | T | 98.0%
| 189.6
/ ‑2.9
| 50 | intergenic (‑440/‑233) | yjiC/iraD | uncharacterized protein/RpoS stabilzer after DNA damage, anti‑RssB factor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (25/24); total (25/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 |
ATCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGG > NC_000913/4556612‑4556905
|
aTCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑t < 1:758139/150‑1 (MQ=255)
ttATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑ttttttt < 2:338346/150‑1 (MQ=255)
ttATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑ttttttt < 2:376397/150‑1 (MQ=255)
atatTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑tttttttttt < 2:704908/151‑2 (MQ=255)
attTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑tttttttttttgagt < 1:177573/151‑7 (MQ=255)
ttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGttt < 1:525851/150‑1 (MQ=255)
ttACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTtg > 1:449610/1‑151 (MQ=255)
aaCTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTgatgat > 1:325279/1‑151 (MQ=255)
gTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCa < 1:757777/151‑1 (MQ=255)
tCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTTCCACCGTAATTAAGTAGCAGCGGTGACAAGTATTTTAATCTACGTAACTATTCGGAATATTTAGAATATACTAGCGGT‑TTTTTTTTTTAGTTTGATGATTTTTTTCaaa > 2:700767/1‑150 (MQ=255)
aataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATTTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGa < 2:194456/150‑1 (MQ=255)
aataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGa < 2:539728/150‑1 (MQ=255)
ataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACt > 2:375693/1‑151 (MQ=255)
taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtt > 2:404379/1‑151 (MQ=255)
tGGGTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtttttt > 1:84790/1‑151 (MQ=255)
tGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACtttttt > 2:184573/1‑151 (MQ=255)
gCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTcc < 1:18844/151‑1 (MQ=255)
gATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCt < 2:838070/149‑1 (MQ=255)
gATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTg > 2:840473/1‑150 (MQ=255)
tATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaaa < 2:884955/150‑1 (MQ=255)
gCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGt > 1:475244/1‑150 (MQ=255)
cGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc > 2:298199/1‑150 (MQ=255)
ggAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc > 1:218768/1‑149 (MQ=255)
gAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGt < 1:401589/151‑1 (MQ=255)
aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat < 1:185693/151‑1 (MQ=255)
aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat < 1:886423/150‑1 (MQ=255)
aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGatat < 2:900184/151‑1 (MQ=255)
aaataaTTTCCCACCGGAATTAAGTAGCAGCCGTGGAAAGTATTTTCATCTACGTAACTAATCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGata < 2:155370/151‑1 (MQ=255)
cACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCaa < 2:16246/151‑1 (MQ=255)
cACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCaa < 1:473208/151‑1 (MQ=255)
cGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGt > 1:494253/1‑150 (MQ=255)
gAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAg < 1:22559/151‑1 (MQ=255)
aaGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGGGTTTGATGATTTTTATAAAATAATAAATTTTTTCCTGAAAAGTAGGTAGTTATTCAAAGGAAAGTAAGGttt > 1:417917/1‑150 (MQ=255)
gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat < 2:449060/151‑1 (MQ=255)
agcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACttt < 1:758278/151‑1 (MQ=255)
aTTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTTCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGAtt > 2:314283/1‑150 (MQ=255)
aTTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTc < 1:720574/151‑1 (MQ=255)
ttttTATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTTTGTGTTTTATGGTTTTTTTCAAATCATGCCTTTTTTCCTTAAAAGTCAGTAGATATTCATAGGCAAGAAAGGCTTTATACTTTTCTGACAGGAtt > 2:545623/1‑150 (MQ=255)
ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAg < 1:559152/150‑1 (MQ=255)
tCTACGTAACTATTCAGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTg > 2:385852/1‑151 (MQ=255)
cTACGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGt > 2:882792/1‑151 (MQ=255)
aCGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct > 1:461764/1‑151 (MQ=255)
aCGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtc > 1:270363/1‑150 (MQ=255)
aCGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGt < 2:494204/149‑1 (MQ=255)
cGTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtctc < 1:679738/151‑1 (MQ=255)
gTAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa < 2:690021/151‑1 (MQ=255)
tAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATATGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa < 2:482860/150‑1 (MQ=255)
tAACTATTCGGAATATTCAGAATATACTAGCGGT‑TTTTTTTTTGAGCTTGATGATTTTTATCAAATAATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATCGGCAAGTAAGGGTTTATACTTTGCTGACAGGATTCGGGCATGtacc > 1:218996/1‑146 (MQ=255)
atTCAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGt < 1:370438/151‑1 (MQ=255)
cAGAATATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAAt > 1:823527/1‑151 (MQ=255)
aaTATACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGa > 1:218285/1‑150 (MQ=255)
atatACTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAAc < 2:420068/151‑1 (MQ=255)
taCTAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACaa > 2:775075/1‑150 (MQ=255)
tAGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTTAAAAGTCATTCGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACACAAgg > 2:741187/1‑151 (MQ=255)
aGCGGT‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAggg > 1:59980/1‑151 (MQ=255)
|
ATCAATTTATATTATTTACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑‑TTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATGAACAAAAGGG > NC_000913/4556612‑4556905
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
