Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,559,131	C→T	M132I (ATG→ATA)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,131	0	C	T	100.0%	170.7 / NA	44	M132I (ATG→ATA)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/18); total (26/18)

CATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGG  >  NC_000913/4558984‑4559274

cATATTGGCGAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATc                                                                                                                                              <  1:455967/151‑1 (MQ=255)

   atTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCa                                                                                                                                            <  2:308204/150‑1 (MQ=255)

            gTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATg                                                                                                                                  >  1:747177/1‑151 (MQ=255)

               aTAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGGCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGcc                                                                                                                                <  1:126026/150‑1 (MQ=255)

                  aaCGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCcttct                                                                                                                            <  2:142481/151‑1 (MQ=255)

                       ccGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCAtt                                                                                                                       <  2:91877/151‑1 (MQ=255)

                                   aaCGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATc                                                                                                                                              >  1:483021/1‑116 (MQ=255)

                                   aaCGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATc                                                                                                                                              <  2:483073/116‑1 (MQ=255)

                                           tCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCg                                                                                                    >  1:169296/1‑150 (MQ=255)

                                               agaTGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAg                                                                                               <  1:653298/151‑1 (MQ=255)

                                                gaTGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAgg                                                                                              >  2:648703/1‑151 (MQ=255)

                                                   ggCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGAt                                                                                           >  2:266901/1‑151 (MQ=255)

                                                           tCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTg                                                                                   >  1:319541/1‑151 (MQ=255)

                                                            cACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTgg                                                                                  >  1:219598/1‑151 (MQ=255)

                                                             aCGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGc                                                                                 >  1:574409/1‑151 (MQ=255)

                                                             aCGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGc                                                                                 >  2:636789/1‑151 (MQ=255)

                                                                     cacaCGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                                                          <  1:88571/150‑1 (MQ=255)

                                                                     cacaCGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATa                                                                         <  2:238794/151‑1 (MQ=255)

                                                                      acacGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                                                          >  2:420625/1‑149 (MQ=255)

                                                                       cacGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGAt                                                                          >  1:192635/1‑148 (MQ=255)

                                                                            tcgatAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTc                                                                    <  2:88448/149‑1 (MQ=255)

                                                                                         cGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGacc                                                     <  2:393525/151‑1 (MQ=255)

                                                                                              ttttCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacc                                                 >  1:658531/1‑150 (MQ=255)

                                                                                               tttCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacca                                                <  2:362062/150‑1 (MQ=255)

                                                                                               tttCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGaccaacca                                                <  1:136929/150‑1 (MQ=255)

                                                                                                 tCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtg                                             >  1:412843/1‑151 (MQ=255)

                                                                                                     cGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacg                                          >  2:110107/1‑150 (MQ=255)

                                                                                                      ggAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtg                                        >  1:412953/1‑151 (MQ=255)

                                                                                                       gAACCCGTAACGGGGCGGGGAGGGACATGAAAAGCTCCGGACAGTATCCAGGCGCTTATGGCTTCTTCATTGAGCGCTCGGGTCTAGGCGAGCAGGGATTGCGGGTGGAGAGAGATAGAGTCGGAGCCCAGCAGACCAACCACtgacgt                                         >  1:108511/1‑149 (MQ=255)

                                                                                                        aaCCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACtgacgtgac                                      >  1:96442/1‑151 (MQ=255)

                                                                                                            cGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGccc                                  >  1:84062/1‑151 (MQ=255)

                                                                                                              tAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGc                                >  1:371003/1‑151 (MQ=255)

                                                                                                                aTGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCtt                              <  1:396703/151‑1 (MQ=255)

                                                                                                                 tGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTc                             >  1:374116/1‑151 (MQ=255)

                                                                                                                 tGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTc                             >  1:66363/1‑151 (MQ=255)

                                                                                                                    tGCGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGt                          >  1:261160/1‑151 (MQ=255)

                                                                                                                      cGGGAAGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCa                        <  2:371050/151‑1 (MQ=255)

                                                                                                                          aaGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCg                    <  2:139307/151‑1 (MQ=255)

                                                                                                                           aGGGACATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCg                    >  1:510316/1‑150 (MQ=255)

                                                                                                                              gACATGATAAGCGCCGGTCAGTGTCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACtt                <  2:218218/151‑1 (MQ=255)

                                                                                                                                cATGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACtt                >  2:379919/1‑149 (MQ=255)

                                                                                                                                 aTGATAAGCGCCGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAgc             >  2:293485/1‑151 (MQ=255)

                                                                                                                                            cGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCgg  <  1:544042/151‑1 (MQ=255)

                                                                                                                                            cGGTCAGTATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCgg  >  2:236024/1‑151 (MQ=255)

CATATTGGCCAGGTGATAAACGTCCGGTGCGGCAGAACGGTGATCAGAGATGGCGCATTTCACGCCAATCACACGATCGATAATCGCCACGTCTTTTTCCACGGAACCCGTAATGGTGCGGGAAGGGACATGATAAGCGCCGGTCAGCATCCAGGCGCTGATGCCTTCTTCATTGAGCGCACGGGTCTTGGCGAGCAGGGATTCCGGGTGGCGAGAGATAGAGTCGGTGCCCAGCAGACCAACCACTGACGTGACGCCCGCTTCCGTCAGGCGACTTAGCGCCACTTCCGG  >  NC_000913/4558984‑4559274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A