Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 257 49.9 1554294 99.0% 1538751 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,626,537 T→C G89G (GGA→GGG)  yhhJ ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,626,5370TC100.0% 152.7 / NA 43G89G (GGA→GGG) yhhJputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/21);  total (22/21)

TCTGGATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGTAACGG  >  NC_000913/3626392‑3626682
                                                                                                                                                 |                                                                                                                                                 
tCTGGATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGc                                                                                                                                              >  1:2619/1‑151 (MQ=255)
   ggATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGcatc                                                                                                                                           >  1:386776/1‑151 (MQ=255)
            cATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATc                                                                                                                                  >  1:330948/1‑151 (MQ=255)
               tGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATcgg                                                                                                                                <  1:725989/150‑1 (MQ=255)
                 ccGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATcggcgg                                                                                                                             >  2:610264/1‑151 (MQ=255)
                  cGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGt                                                                                                                            <  2:495954/151‑1 (MQ=255)
                               gCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCgg                                                                                                                >  1:303768/1‑150 (MQ=255)
                                  cATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTgg                                                                                                             <  1:472/150‑1 (MQ=255)
                                     gcgcGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGTGGCaa                                                                                                          >  2:237989/1‑150 (MQ=255)
                                           ggCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCAc                                                                                                   <  2:274972/151‑1 (MQ=255)
                                            gCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATTATCTCCCGTGGCaaaaaa                                                                                                      >  1:625226/1‑146 (MQ=255)
                                              aTCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGa                                                                                                <  1:263143/151‑1 (MQ=255)
                                                cGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGTGGCAAAAACCACgg                                                                                                 >  1:515734/1‑148 (MQ=255)
                                                  aCGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATTTCCCGGGGCAAAAACCACgg                                                                                                 >  2:395611/1‑146 (MQ=255)
                                                    gTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAg                                                                                          <  2:235813/151‑1 (MQ=255)
                                                    gTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGGGATTATCTCCGGGGGCAAAAAacac                                                                                                   >  2:11943/1‑142 (MQ=255)
                                                        aCCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGGGATCATCTCCCGGGGCAAAAAACACGGACGAt                                                                                            >  1:665934/1‑145 (MQ=255)
                                                            gAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTg                                                                                    <  1:135581/149‑1 (MQ=255)
                                                                        ggCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAACCCACGGACGATAGAAGCTGTTAACGATCCGGtt                                                                      <  2:395255/151‑1 (MQ=255)
                                                                        ggCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCAGCAGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGCTCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGtt                                                                      <  1:325265/151‑1 (MQ=255)
                                                                             ccGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGAt                                                                  <  2:464233/150‑1 (MQ=255)
                                                                                  gAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAACCACGGGCGATAGAAGCTGTTAAAGATCCGGTTCGATAAATGc                                                            >  2:561654/1‑151 (MQ=255)
                                                                                      aCATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATTTCCCGTGGCAAAAACCACGAGCGATAAAAGCTGTTAACGGTCCGGTTTGATAACTGCGAtt                                                        >  2:127569/1‑151 (MQ=255)
                                                                                      aCATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGAtt                                                        <  2:571350/151‑1 (MQ=255)
                                                                                          cACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATTATCTCCGGGGGCAAAAAACACGGACGATAGAAACTGTTTACGATCCGGGTCGATAAATGCGAt                                                         >  1:403166/1‑146 (MQ=255)
                                                                                              cTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAAACCCGGACGAAAAAAGCTGGTAAAGAACCCGTTCGACAACTGCGATTGATCCa                                                  >  1:671538/1‑149 (MQ=255)
                                                                                                 aaaaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCg                                             <  2:88869/151‑1 (MQ=255)
                                                                                                 aaaaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAACCCCGGGAGATAGAAGCCGGTAAAGAACCGGGTCGATAACTGCGATTGATCCatat                                               >  2:220067/1‑149 (MQ=255)
                                                                                                   aaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGc                                           <  1:755592/151‑1 (MQ=255)
                                                                                                   aaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGc                                           <  2:400663/151‑1 (MQ=255)
                                                                                                   aaTTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGGGGGAAAAAACACCGGCGATAGAAGCTGTTTAAGATCCGGTTCGGTAACTGCGCTTGGTTCATATCgg                                            >  1:212497/1‑150 (MQ=255)
                                                                                                       aGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATg                                       <  2:212529/151‑1 (MQ=255)
                                                                                                              atatTTATCGCGAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTAAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCg                                <  1:650274/151‑1 (MQ=255)
                                                                                                                      cgcgAAGGTATAGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGGGGCAAAAAACACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAAATGCGATTGATCCATATCGGCAATGGCGGACGGCGCgggg                        >  2:438504/1‑151 (MQ=255)
                                                                                                                               ataGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGAt               <  2:611425/151‑1 (MQ=255)
                                                                                                                               ataGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAAACACGGACGATAGAAGCTGTTAACGATCCGGGTCGGTAACTGCGGTTGGTCCCTATCGGCAATGGGGATCGGCGCGGGGGTCAACGa                >  2:207124/1‑150 (MQ=255)
                                                                                                                               ataGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGGGGCAAAAAACACGGGCGGTAGAAGCTGTTAACCATCCGGTTCGGTAACTGCGGTTGATCCATATCGGCAATGGCGATCGGGGCGAGGTTCa                    >  2:424281/1‑146 (MQ=255)
                                                                                                                                 aGCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATcc             <  2:713554/151‑1 (MQ=255)
                                                                                                                                  gCGTCCGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCt            <  2:386825/151‑1 (MQ=255)
                                                                                                                                       cGGCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGt       <  2:500416/151‑1 (MQ=255)
                                                                                                                                        ggCGTCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGTa      <  2:640189/151‑1 (MQ=255)
                                                                                                                                           gTCCAGCCCGGCATCCATCTCATCGGCGGTGGTCATATCCCGGGGCAAAAAACACGGACGGTAGAAGCTGTTTACGATCCGGTTCGATAACTGCGGATTATCCCTATCGGCAATGGGGGTCGGCGCGAGGGTCAAAGATCCTGGCGTaa     >  1:533436/1‑149 (MQ=255)
                                                                                                                                            tCCAGCCCGGCATCCATCTCATCGGCGGTGATCATCTCCCGTGGCAAAAACCACCGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCCTATCGGCAATGGCGATCGGCGCGAGGGTCAACGATCCTGGCGTAAAgg  >  1:265410/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TCTGGATATACCCATTGCCGGTAAATGCCTGGCTCATGCGCGTGGCATCGACGTTCACCTGAATATCCGGCTGGCGTCCGGCGAGGACATCACGCTGAAAATTAGGCGGAATATTTATCGCGAAGGTATAGCGTCCGGCGTCCAGTCCGGCATCCATCTCATCGGCGGTGATCATCTCCGGTGGCAAAAACCACGGACGATAGAAGCTGTTAACGATCCGGTTCGATAACTGCGATTGATCCATATCGGCAATGGCGATCGGCGCGAGGTTCAACGATCCTGGCGTAACGG  >  NC_000913/3626392‑3626682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A