Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,636,796	C→A	Q205H (CAG→CAT)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,796	0	C	A	100.0%	133.8 / NA	37	Q205H (CAG→CAT)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/21); total (16/21)

AATTCCCCCGGTTGCC‑AGTAGCTTGAAATCTGCAACACCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGC  >  NC_000913/3636656‑3636939

aaTTCCCCCGGTTTCCAAGTGGTTAAAATTCTGAACCCCCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCCCGAAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTa                                                                                                                                             <  2:344854/146‑1 (MQ=255)

      cccGGGTGCC‑AGTTATTTAAAATTCGCCAACCCCCCGGTTCCAAACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGAAAAACGGGGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGc                                                                                                                                  <  2:765419/151‑1 (MQ=255)

         ggTTGCC‑AGTAGCTTGAAATCTGCAACACCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACg                                                                                                                              >  2:347691/1‑151 (MQ=255)

                                       cgccgG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGAccc                                                                                                 >  1:743141/1‑151 (MQ=255)

                                          cgG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcg                                                                                              >  2:827241/1‑151 (MQ=255)

                                            g‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCcgcgcg                                                                                            >  1:348511/1‑151 (MQ=255)

                                                   aCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCgg                                                                                      >  2:295587/1‑151 (MQ=255)

                                                   aCAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGAAAACCGGTGCCGTTTTCAGCGGT‑AACCACGAAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTc                                                                                        <  2:439702/149‑1 (MQ=255)

                                                    cAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGgc                                                                                     >  1:514123/1‑151 (MQ=255)

                                                      acccccgGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCCGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGt                                                                                         >  1:425007/6‑145 (MQ=255)

                                                         cgcgGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCAc                                                                                >  1:531727/1‑151 (MQ=255)

                                                            ggtgggtgAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                                                                             >  2:534468/1‑151 (MQ=255)

                                                            ggtgggtgAAGAGAAAGTTCCCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACg                                                                               <  2:301190/149‑1 (MQ=255)

                                                            ggtgggtaAAGAGTAAGTTCCCAGGAAACCCGCCGCTGTTTACAGCGGC‑ATCCCCGGAAGACCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGtt                                                                             <  2:649306/151‑1 (MQ=255)

                                                                    aaGAGTAAGTTCTCACGGAAACGGGTGCCGTTTTCAGCGTT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGcc                                                                       <  1:213221/149‑1 (MQ=255)

                                                                    aaGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCa                                                                      >  2:738814/1‑150 (MQ=255)

                                                                    aaGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCa                                                                      >  1:7086/1‑150 (MQ=255)

                                                                    aaGAGTAAGTTCCCACGGAAACCGGTGCCGTTTTCAGCGTT‑AACCACGAAAGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                                                                     <  2:648990/151‑1 (MQ=255)

                                                                     agagTAAGTTCGCCCGAAAACGGGTCCCGTTTACACCGGT‑AGCCACGGAAGTCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCaa                                                                     <  2:202016/150‑1 (MQ=255)

                                                                          agtttccccgggAAAACGGTGCCGTTTTCAGCGTT‑AATCACGGAAGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGt                                                                <  1:54697/140‑1 (MQ=255)

                                                                          aaGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGAAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                                                               <  2:601489/151‑1 (MQ=255)

                                                                          aaGTTCTCACGAAAACCGGTCCCTTTTTCGGCGGT‑AATCCGGGAGGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGtt                                                               <  2:319352/151‑1 (MQ=255)

                                                                           attcctgaCGAAATACTGTCCCTTTTTCTTCTGT‑AATCGGGGACGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCCACGCTTCACGCAGAGTCAATGCCCCCACACCCCCCCGCGTCCGCAGCCCGTTGAGGCCAAAtt                                                                  <  1:200773/140‑1 (MQ=255)

                                                                                tcACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCa                                                          >  2:153951/1‑150 (MQ=255)

                                                                                 cACGGAAAAGGGTGCCGTTTCCAGCGGT‑AACCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAAt                                                        <  2:531764/151‑1 (MQ=255)

                                                                                      aaaaGGGTGCC‑TTTTCCGGGGTAAATCACGAAAGCCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATc                                                       <  1:336543/147‑1 (MQ=255)

                                                                                      aaaaCGGTCCCTTTTTAGGGGGT‑AATCCCGAAGGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTtat                                                   <  1:558855/151‑1 (MQ=255)

                                                                                           ggTGCCTTTTTCAGCGTT‑AATCCCGAAGGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAAc                                                <  2:602419/149‑1 (MQ=255)

                                                                                           ggTCCCTTTTCCGGCGGA‑AACCACGGAAGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAAcc                                               <  1:23449/150‑1 (MQ=255)

                                                                                               ccGTTTCCAGGGTA‑AACCACGAAGGGCCCCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCaaa                                            <  1:625115/149‑1 (MQ=255)

                                                                                                cGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACTCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGAGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACg                                          >  1:94167/1‑150 (MQ=255)

                                                                                                 gTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGc                                        >  1:283833/1‑151 (MQ=255)

                                                                                                          gtt‑AATCCGGGAAGGCACCGCCCCGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGa                                      <  2:803998/142‑1 (MQ=255)

                                                                                                                    ggAAGGCACCGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGc                      >  2:663222/1‑151 (MQ=255)

                                                                                                                        gcccccGCCACGCCCGCCAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTg                  <  1:534431/147‑1 (MQ=255)

                                                                                                                                      cgccAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTc    <  2:438311/151‑1 (MQ=255)

                                                                                                                                        ccAGCACATGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTcgc  >  1:224223/1‑151 (MQ=255)

AATTCCCCCGGTTGCC‑AGTAGCTTGAAATCTGCAACACCGCCGG‑TCCAGACAAGCCGCGGTGGGTGAAGAGTAAGTTCTCACGGAAAACGGTGCCGTTTTCAGCGGT‑AATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGC  >  NC_000913/3636656‑3636939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A