Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	257	49.9	1554294	99.0%	1538751	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,887,033	G→A	G69D (GGC→GAC)	mnmE →	tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,887,033	0	G	A	100.0%	147.6 / NA	40	G69D (GGC→GAC)	mnmE	tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/16); total (24/16)

CATCCTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGGCCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAACGCGCGTTTCT  >  NC_000913/3886887‑3887177

cATCCTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGAccc                                                                                                                                               >  1:563736/1‑150 (MQ=255)

   ccTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAAc                                                                                                                                           <  2:603106/151‑1 (MQ=255)

      gcgcATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACt                                                                                                                                          <  1:287465/149‑1 (MQ=255)

      gcgcATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTc                                                                                                                                         <  2:769684/150‑1 (MQ=255)

              ccGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCg                                                                                                                                 <  1:485222/150‑1 (MQ=255)

                  cTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGa                                                                                                                             >  2:418340/1‑150 (MQ=255)

                    tCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGa                                                                                                                             >  2:293298/1‑148 (MQ=255)

                     cAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGa                                                                                                                             >  2:175148/1‑147 (MQ=255)

                       aaGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGAtgt                                                                                                                       >  1:550244/1‑151 (MQ=255)

                          cccGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCt                                                                                                                    >  2:454759/1‑151 (MQ=255)

                          cccGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCt                                                                                                                    <  1:245964/151‑1 (MQ=255)

                                   ttGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGc                                                                                                            >  1:649638/1‑150 (MQ=255)

                                          aaCCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCAt                                                                                                    >  1:46482/1‑151 (MQ=255)

                                           aCCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATg                                                                                                   <  1:353531/151‑1 (MQ=255)

                                              gTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCg                                                                                                <  1:407335/151‑1 (MQ=255)

                                                gCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGt                                                                                              >  2:243677/1‑151 (MQ=255)

                                                       aaaaTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCgggg                                                                                        >  1:500616/1‑148 (MQ=255)

                                                           tGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATcc                                                                                    >  2:114707/1‑150 (MQ=255)

                                                                    cgcgCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACctgct                                                                          <  2:724548/151‑1 (MQ=255)

                                                                        cTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGtt                                                                       <  2:586565/150‑1 (MQ=255)

                                                                         tACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTa                                                                      >  1:211349/1‑150 (MQ=255)

                                                                          aCGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTaa                                                                     >  2:660424/1‑150 (MQ=255)

                                                                           cGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTaaaa                                                                   <  2:645403/151‑1 (MQ=255)

                                                                           cGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCCCGACCTGCTGTTaaa                                                                    >  1:276376/1‑150 (MQ=255)

                                                                                aTTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCa                                                               >  2:472983/1‑150 (MQ=255)

                                                                                aTTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCAt                                                              >  2:595146/1‑151 (MQ=255)

                                                                                   aTCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTc                                                            <  2:74103/150‑1 (MQ=255)

                                                                                          gTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGATTCCGTTCACCGGCGAAGATGTGCTGGAACTGCCAGCTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGAccct                                                     <  1:499119/150‑3 (MQ=255)

                                                                                             tAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGGGATCCTCGACCTGCTGTTAAAACGCATTCTGACCAATccc                                                 >  1:653286/1‑151 (MQ=255)

                                                                                              aaaGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCg                                                <  1:400280/151‑1 (MQ=255)

                                                                                               aaGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTccc                                                 >  2:755340/1‑149 (MQ=255)

                                                                                                  aCGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGAACTGCTGTTAAAACGAATTCTGACCATTCCCGGcc                                             >  1:491603/1‑150 (MQ=255)

                                                                                                                  gCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGGAGATGTGCTGGAACTGCAAGGTCATGGCGGGTCCGAGATCCTCGAACTGCTGTTAAAACGCACTCTGATCCTTCCCGGCCTGCGGATTGCTAGccc                             >  1:624023/1‑150 (MQ=255)

                                                                                                                         cAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAg                      >  2:470588/1‑150 (MQ=255)

                                                                                                                           ggggATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGttt                   <  1:188025/151‑1 (MQ=255)

                                                                                                                                    gcTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAAcg           >  2:331298/1‑150 (MQ=255)

                                                                                                                                     cTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAAcgc          >  2:336027/1‑150 (MQ=255)

                                                                                                                                       aTGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAAcgcgcg       <  1:114675/151‑1 (MQ=255)

                                                                                                                                             cccTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAACGCGCGTTTCt  <  2:20626/150‑1 (MQ=255)

                                                                                                                                               cTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCAATCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAACGCGCGGTTCt  >  1:440226/1‑148 (MQ=255)

CATCCTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGGCCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAACGCGCGTTTCT  >  NC_000913/3886887‑3887177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A