Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,887,033	G→A	G69D (GGC→GAC)	mnmE →	tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,887,033	0	G	A	100.0%	157.0 / NA	43	G69D (GGC→GAC)	mnmE	tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/18); total (25/18)

GGCATCCTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGGCCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAACGCGCGTTTCTT  >  NC_000913/3886885‑3887178

ggCATCCTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGAcc                                                                                                                                                 >  1:345927/1‑151 (MQ=255)

  cATCCTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCg                                                                                                                                               <  2:55111/151‑1 (MQ=255)

         cgcATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGt                                                                                                                                        >  2:239514/1‑151 (MQ=255)

                 cGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGc                                                                                                                                <  1:145458/151‑1 (MQ=255)

                      tCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAg                                                                                                                            <  1:556141/150‑1 (MQ=255)

                       cAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGa                                                                                                                              >  1:850799/1‑147 (MQ=255)

                          aGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGAtgtg                                                                                                                       >  1:599433/1‑151 (MQ=255)

                            cccGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCt                                                                                                                     >  2:11167/1‑151 (MQ=255)

                               gTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTg                                                                                                                    <  2:836529/149‑1 (MQ=255)

                               gTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTg                                                                                                                    >  1:836471/1‑149 (MQ=255)

                                  aaGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACt                                                                                                               <  2:548797/151‑1 (MQ=255)

                                   aGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTg                                                                                                              <  2:427986/151‑1 (MQ=255)

                                     ttGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGc                                                                                                             >  1:759750/1‑150 (MQ=255)

                                       gCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAg                                                                                                          <  2:405026/151‑1 (MQ=255)

                                         cGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGt                                                                                                        <  1:47824/151‑1 (MQ=255)

                                          gAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTc                                                                                                       >  1:283563/1‑151 (MQ=255)

                                            aaCCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCAt                                                                                                     >  1:152982/1‑151 (MQ=255)

                                                gTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCg                                                                                                 <  1:829426/151‑1 (MQ=255)

                                                             tGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATcc                                                                                     >  1:586318/1‑150 (MQ=255)

                                                                  aaGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGAcc                                                                               >  2:273891/1‑151 (MQ=255)

                                                                    gCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACctg                                                                             <  1:443888/151‑1 (MQ=255)

                                                                        cgcTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGGGATCCTAGACCTGCTGt                                                                         >  2:626635/1‑151 (MQ=255)

                                                                            aCGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGCCCTGCTGTTa                                                                       <  1:225113/149‑1 (MQ=255)

                                                                              gCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTaaaa                                                                    >  2:607115/1‑150 (MQ=255)

                                                                                cGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGAGATCCTCGACCTGCTGTTAAAACGc                                                                 >  1:332226/1‑151 (MQ=255)

                                                                                         tCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCt                                                            <  1:307325/147‑1 (MQ=255)

                                                                                         tCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGAcc                                                        >  1:14567/1‑151 (MQ=255)

                                                                                             tttAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAAGTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGACCGGGGAACCTCCAACTGCTGTTTAAACGAACTATGACCAtt                                                     >  1:188120/1‑150 (MQ=255)

                                                                                              ttAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTcc                                                   >  2:120422/1‑151 (MQ=255)

                                                                                                aaaGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTccc                                                  <  1:484573/150‑1 (MQ=255)

                                                                                                 aaGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGGGATTCTCGACCTGCTGTTAAAACGCATTCTGACCATTTCCgg                                                >  1:129411/1‑151 (MQ=255)

                                                                                                    aCGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCt                                             <  2:383535/151‑1 (MQ=255)

                                                                                                         gACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCgg                                         >  2:129123/1‑150 (MQ=255)

                                                                                                                    gCTCGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGGGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGccc                              >  1:773949/1‑150 (MQ=255)

                                                                                                                       cGATCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTgg                           >  2:421918/1‑150 (MQ=255)

                                                                                                                          tCAGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTg                         >  2:486683/1‑149 (MQ=255)

                                                                                                                            aGGGGATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGtt                     <  1:392778/151‑1 (MQ=255)

                                                                                                                                gATTGCGCTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGGCCGGTGCTCCTCGACCTGCTGTTAAAACGCATTCTGACCCTTCCCGGCCTGCGGATTGCTCGCCCCGGTTGGGTTTcc                 >  1:827306/1‑151 (MQ=255)

                                                                                                                                    gcgcTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAAc             <  2:606039/151‑1 (MQ=255)

                                                                                                                                      gcTATGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAAcgc           <  2:425535/151‑1 (MQ=255)

                                                                                                                                         aTGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAAcgcgcg        <  1:667307/151‑1 (MQ=255)

                                                                                                                                         aTGGTTCCCTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCCGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGGGAGTTTTCCGAAcgcgcg        >  1:752963/1‑151 (MQ=255)

                                                                                                                                                 cTGACCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGGGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAACGCGCGTTTCtt  >  1:413452/1‑149 (MQ=255)

GGCATCCTGCGCATCTCCGGCTTCAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACGCCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGGCCCGAACTCGTTCACCGGCGAAGATGTGCTGGAACTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCGCCCTGGTGAGTTTTCCGAACGCGCGTTTCTT  >  NC_000913/3886885‑3887178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A