Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F50 I1 R1
|
300 |
54.7 |
1718999 |
98.4% |
1691495 |
149.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,457,566 |
C→T |
A100A (GCG→GCA) |
yjgA ← |
ribosome‑associated UPF0307 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,457,566 | 0 | C | T | 98.0%
| 179.3
/ NA
| 50 | A100A (GCG→GCA) | yjgA | ribosome‑associated UPF0307 family protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (28/21); total (29/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGT > NC_000913/4457420‑4457711
|
cGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTACTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCt < 2:192707/151‑1 (MQ=255)
gCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAAt > 1:737334/1‑151 (MQ=255)
tGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAAt > 2:77875/1‑149 (MQ=255)
cGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGc < 1:299147/150‑1 (MQ=255)
tCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGctct > 2:804280/1‑150 (MQ=255)
ggCATCGGGCCACAGATTTAATCCCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATGGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGTTTGTGACGG‑TTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGt < 1:503226/151‑1 (MQ=255)
cATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTc < 2:552699/151‑1 (MQ=255)
gggCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCgcgc < 1:277563/151‑1 (MQ=255)
ccACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTgg < 2:756984/151‑1 (MQ=255)
acaGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGgcg > 2:636925/1‑151 (MQ=255)
aTACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCCGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCt > 1:794824/1‑150 (MQ=255)
aCCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTAc > 2:381699/1‑151 (MQ=255)
tCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCg > 2:476790/1‑150 (MQ=255)
gCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATg > 2:485863/1‑150 (MQ=255)
gCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGa < 2:154783/151‑1 (MQ=255)
ggCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGGTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGGCGCGCTGGCGCAGCATCTTACCGATGAGCTGc > 2:636962/1‑151 (MQ=255)
gCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCa < 1:223045/151‑1 (MQ=255)
gTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAAc < 1:15272/151‑1 (MQ=255)
atcaCCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTgg > 1:695103/1‑151 (MQ=255)
aCCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggcgg > 1:561966/1‑151 (MQ=255)
ccTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATGCAGTGCCTGACGAATAGGCTCTTCGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTggctgc < 1:327545/151‑1 (MQ=255)
gatcgatcAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGgcggcggcg > 2:429239/1‑151 (MQ=255)
cgatcAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAcc > 2:315451/1‑151 (MQ=255)
cgatcAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGAcc < 2:715934/151‑1 (MQ=255)
cAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTc < 2:202638/150‑1 (MQ=255)
gACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCAt > 2:95471/1‑151 (MQ=255)
aCGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATc > 2:635745/1‑151 (MQ=255)
aaTCGCGCAGGATTTCTAGTTTATGGAAGAGCACCACCCGCTGGTTGTGACGGAATTTCAGCTTATCCAGCGCCTGACGAATAGTCTCTACGTCGCGCTGGCCCAGCATCTTCCCGTTGACCTGCAACTGGCGGCGGCGACCTTCCATc > 1:706815/2‑149 (MQ=255)
cgcgCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAAt < 2:782059/151‑1 (MQ=255)
ttCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGc < 1:369187/150‑1 (MQ=255)
ccAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCg > 1:68997/1‑149 (MQ=255)
cAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGc > 2:734270/1‑151 (MQ=255)
ttATGGAACAGCACCACCTGCTGGTTGTGACGGCTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCAATGAGCTGCAACTGGCGGCGGCGACCTTCCCTCTTAATACGCTGGGCGAGCTCa > 1:201769/1‑150 (MQ=255)
aTGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAAt < 2:206807/150‑1 (MQ=255)
aTGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAAt > 2:252037/1‑150 (MQ=255)
aTGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATa < 2:700257/151‑1 (MQ=255)
gAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGcc < 2:235975/151‑1 (MQ=255)
gCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCcgcgcg < 1:216450/151‑1 (MQ=255)
ccTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCgcgcgc > 2:344602/1‑146 (MQ=255)
ccTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCgcgcgc > 2:220157/1‑146 (MQ=255)
tgctgGTTGTGACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCg > 1:200632/1‑151 (MQ=255)
gtgACGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTaa < 1:175764/150‑1 (MQ=255)
aCGGTTTTTCAGCTTATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACggg > 2:384631/1‑151 (MQ=255)
gTTTTTCAGCTTATCCAGTGCCTGACGTATCGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGCCCTTCCATCTTAATACGTTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATc > 1:429804/1‑151 (MQ=255)
ttATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgcg > 1:815608/1‑151 (MQ=255)
tATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgcgc > 1:250894/1‑151 (MQ=255)
tATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgcg < 1:635703/150‑1 (MQ=255)
tATCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAgc < 1:350389/149‑1 (MQ=255)
aTCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGcgcg > 1:183409/1‑151 (MQ=255)
tCCAGTGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGt > 1:378088/1‑151 (MQ=255)
|
CGCAGTTGCTGACGATCGGCATCGGGCCACAGATTTAATACCTCAGCGATGGCGTCATCACCTTGATCGATCAGACGATCGCGCAGGTTTTCCAGTTTATGGAACAGCACCACCTGCTGGTTGTGACGGTTTTTCAGCTTATCCAGCGCCTGACGAATAGGCTCTACGTCGCGCTGGCGCAGCATCTTACCGATGAGCTGCAACTGGCGGCGGCGACCTTCCATCTTAATACGCTGGGCGAGCTCAATAGCCGCGCGCAGATCCGCATCTAACGGGATCTTATCCAGCGCGT > NC_000913/4457420‑4457711
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
