Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I1 R1	300	54.7	1718999	98.4%	1691495	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,550,166	A→G	T377T (ACT→ACC)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,550,166	0	A	G	100.0%	152.2 / NA	43	T377T (ACT→ACC)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/25); total (18/25)

GGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAC  >  NC_000913/4550025‑4550307

ggTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTgg                                                                                                                                       >  2:505059/1‑150 (MQ=255)

 gTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGAt                                                                                                                                     >  2:172076/1‑151 (MQ=255)

        aCGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGa                                                                                                                               >  1:498952/1‑150 (MQ=255)

         cGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGAtt                                                                                                                             <  1:543827/151‑1 (MQ=255)

                tttACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCAc                                                                                                                      >  1:566231/1‑151 (MQ=255)

                tttACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCAc                                                                                                                      >  2:221916/1‑151 (MQ=255)

                tttACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCAc                                                                                                                      >  2:497319/1‑151 (MQ=255)

                     tGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGAt                                                                                                                  >  1:365661/1‑150 (MQ=255)

                       aCAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAAt                                                                                                               >  1:236426/1‑151 (MQ=255)

                           gTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcg                                                                                                           <  2:61135/151‑1 (MQ=255)

                              aaaGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTgca                                                                                                                          <  2:304968/133‑1 (MQ=255)

                              aaaGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTgca                                                                                                                          >  1:304943/1‑133 (MQ=255)

                              aaaGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcggcg                                                                                                        <  2:56548/151‑1 (MQ=255)

                                   aaCCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCcggcggcgg                                                                                                    <  1:588247/150‑1 (MQ=255)

                                        ttGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCg                                                                                              >  1:694344/1‑151 (MQ=255)

                                           aacaacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCg                                                                                           >  1:591356/1‑151 (MQ=255)

                                             caacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGaa                                                                                         <  1:193277/151‑1 (MQ=255)

                                             caacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGa                                                                                          >  1:497207/1‑150 (MQ=255)

                                              aacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGaaa                                                                                        <  2:485109/151‑1 (MQ=255)

                                                          gCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGAc                                                                            >  2:30351/1‑151 (MQ=255)

                                                            aTCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGAccc                                                                          <  2:666401/151‑1 (MQ=255)

                                                              cGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGt                                                                        <  2:185563/151‑1 (MQ=255)

                                                               gTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTc                                                                       <  1:5959/151‑1 (MQ=255)

                                                                     gtgGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAg                                                                  <  1:497283/150‑1 (MQ=255)

                                                                           gAGGGTGTTGGAATCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAAt                                                            <  1:127553/150‑1 (MQ=255)

                                                                                      aaCCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGAt                                                <  2:110457/151‑1 (MQ=255)

                                                                                       aCCCGCAGCCGTCGCCAGTACCAGCAGCACCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGAt                                                <  1:672226/150‑1 (MQ=255)

                                                                                         ccGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATc                                               >  1:314029/1‑149 (MQ=255)

                                                                                                   cGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgccat                                    <  1:453231/150‑1 (MQ=255)

                                                                                                   cGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGccatgccatg                                   >  2:295388/1‑151 (MQ=255)

                                                                                                   cGCCAGTACCAGCAGCGACGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAgccatgccat                                    <  2:114399/150‑1 (MQ=255)

                                                                                                     ccAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCatgat                                 <  1:161670/151‑1 (MQ=255)

                                                                                                      cAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCatgatg                                <  2:255138/151‑1 (MQ=255)

                                                                                                          aCCAGCAGCGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAgg                            >  2:492635/1‑151 (MQ=255)

                                                                                                                agcGCCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCg                         >  2:167856/1‑148 (MQ=255)

                                                                                                                 gcgcCGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGat                     <  2:486939/151‑1 (MQ=255)

                                                                                                                    ccGGATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATAtt                  <  2:515333/151‑1 (MQ=255)

                                                                                                                      ggATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGc                <  1:1630/151‑1 (MQ=255)

                                                                                                                      ggATTCACGCCAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGc                <  2:764309/151‑1 (MQ=255)

                                                                                                                               ccAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGAc        >  1:669547/1‑150 (MQ=255)

                                                                                                                               ccAACCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACa       <  2:23846/151‑1 (MQ=255)

                                                                                                                                  aCCAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCa    <  1:408823/151‑1 (MQ=255)

                                                                                                                                    cAGCTGACCGGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGCCATCGCCGAAACGACACCCTGACCCGTCGCCAGACTAATTAGCACAGTGATCCGCCATGCCATGATGTAGCGCGTGATATTGCCGTGGGACATCacc  <  1:57829/151‑3 (MQ=255)

GGTTTTCAACGTGTCTTTTACTGACAGGTCAAAGTAACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAC  >  NC_000913/4550025‑4550307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A