Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,244,134 A→G V75V (GTA→GTG)  yeiG → S‑formylglutathione hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,244,1340AG99.3% 503.9 / NA 151V75V (GTA→GTG) yeiGS‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (71/79);  minor base T (0/1);  total (71/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 151 total aligned reads displayed.

GCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAT  >  NC_000913/2243986‑2244278
                                                                                                                                                    |                                                                                                                                                 
  ccGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTgg                                                                                                                                               <  1:408974/151‑1 (MQ=255)
      tGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTg                                                                                                                                             >  1:1603724/1‑149 (MQ=255)
        aCGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGc                                                                                                                                         >  4:409615/1‑151 (MQ=255)
              aGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGac                                                                                                                                    >  4:563542/1‑150 (MQ=255)
              aGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGGGCCCAGAGGGTAGCGGCGGAACTGGGGAGTGTGCCGGTGATTcccggc                                                                                                                                    >  2:133652/1‑146 (MQ=255)
                 aTCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACAcc                                                                                                                                 >  4:1020095/1‑150 (MQ=255)
                  tCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGACCTGGGGATTGTGCTGGTGATGCCAGACAcc                                                                                                                                 <  3:384232/149‑1 (MQ=255)
                  tCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACAcc                                                                                                                                 >  2:460003/1‑149 (MQ=255)
                     ttCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCa                                                                                                                                <  3:153895/147‑1 (MQ=255)
                     ttCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCa                                                                                                                                <  1:1868650/147‑1 (MQ=255)
                      tctcCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGccc                                                                                                                            >  4:1077844/1‑150 (MQ=255)
                             ccACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTTTGCTGGGGATGCCAGACACCAGCCcg                                                                                                                           >  2:51785/1‑144 (MQ=255)
                             ccACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCgcggcg                                                                                                                    <  1:1793560/151‑1 (MQ=255)
                             ccACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCgcgg                                                                                                                      <  4:1758477/149‑1 (MQ=255)
                               aCCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCgcggcg                                                                                                                    >  1:815884/1‑149 (MQ=255)
                                ccTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAGCTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTTTGCTGGTGATGCCAGACACCAGCACGCGCGGCGaaa                                                                                                                 <  1:832578/151‑1 (MQ=255)
                                ccTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGaa                                                                                                                  >  2:544490/1‑150 (MQ=255)
                                   cGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAg                                                                                                               <  1:961212/150‑1 (MQ=255)
                                    gTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGt                                                                                                             <  1:1408026/151‑1 (MQ=255)
                                      gATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTg                                                                                                           <  4:1460559/151‑1 (MQ=255)
                                         cacaCTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGcc                                                                                                         >  1:615056/1‑150 (MQ=255)
                                         cacaCTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCa                                                                                                        <  1:592832/151‑1 (MQ=255)
                                          acacTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCaa                                                                                                       <  4:363745/151‑1 (MQ=255)
                                            acTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAac                                                                                                      >  4:1250655/1‑150 (MQ=255)
                                               ccGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAac‑gacg                                                                                                 <  1:33423/151‑1 (MQ=255)
                                               ccGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAac‑gac                                                                                                  <  1:1480145/150‑1 (MQ=255)
                                                cGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGATGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAac‑gacg                                                                                                 >  2:56121/1‑150 (MQ=255)
                                                  ccaccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATg                                                                                              <  4:867182/151‑1 (MQ=255)
                                                  ccaccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATg                                                                                              <  4:1672532/151‑1 (MQ=255)
                                                  ccaccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATg                                                                                              <  4:1760261/151‑1 (MQ=255)
                                                   caccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACT‑GGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGc                                                                                            <  4:163012/151‑1 (MQ=255)
                                                     ccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCCGACACCAGCCCGCGCGGCGAAAAGGGTGCCAAC‑GACGATGGc                                                                                            >  3:467972/1‑150 (MQ=255)
                                                     ccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCt                                                                                           <  1:1570496/151‑1 (MQ=255)
                                                     ccaGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGGGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGc                                                                                            >  1:1417234/1‑150 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGa                                                                                       <  1:962439/150‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGa                                                                                       <  4:275991/150‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGAt                                                                                      <  1:481803/151‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGAt                                                                                      <  4:1553352/151‑1 (MQ=255)
                                                          gCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGAGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAA‑GACGATGGCTACGAt                                                                                      >  2:414001/1‑151 (MQ=255)
                                                            tGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGGTGCCAAC‑GACGATGGCTACGAttt                                                                                    >  1:739061/1‑151 (MQ=255)
                                                              tACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGAttt                                                                                    >  2:343239/1‑149 (MQ=255)
                                                               aCTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAgg                                                                                 >  2:339487/1‑151 (MQ=255)
                                                               aCTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAg                                                                                  <  1:1129786/150‑1 (MQ=255)
                                                               aCTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAAATGGGGATTGTGCTGGTGATGCCCGACACCAGCCCGCGCGGCGGAAAGGTTGCCAAC‑GACGATGGCTACGATTTAgg                                                                                 >  1:1340840/1‑151 (MQ=255)
                                                                cTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAgg                                                                                 >  2:386147/1‑150 (MQ=255)
                                                                 tGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGcc                                                                               >  4:1360532/1‑151 (MQ=255)
                                                                  ggCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGcc                                                                               >  4:749010/1‑150 (MQ=255)
                                                                   gCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGcc                                                                               >  1:525449/1‑149 (MQ=255)
                                                                     tttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAggg                                                                           >  4:596390/1‑151 (MQ=255)
                                                                      ttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAggg                                                                           <  4:780870/150‑1 (MQ=255)
                                                                      ttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAggg                                                                           >  1:1664896/1‑150 (MQ=255)
                                                                      ttCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAgg                                                                            <  1:282735/149‑1 (MQ=255)
                                                                        ccGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCCGGgcg                                                                         >  1:1903588/1‑150 (MQ=255)
                                                                        ccGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGgcgc                                                                        <  1:922880/151‑1 (MQ=255)
                                                                        ccGGATAAACCTGCAATGACGAGAACGTCGCCACCAAGGCGGGTGCCCAGCGGGCAGGGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGGGCGGCGAAAAGGTTGCCAAC‑TACGATGGCTCCGATTTAGGCCAGGgc                                                                          <  3:52583/149‑1 (MQ=255)
                                                                         cGGATTAACCTGCAATGACGATAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGTTTGCCAAC‑GACGATTGCTACGATTTAGGCCAGGGAGCa                                                                       <  3:390303/151‑1 (MQ=255)
                                                                          ggATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGgcg                                                                         >  4:1463134/1‑148 (MQ=255)
                                                                              tAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCt                                                                   <  2:413871/150‑1 (MQ=255)
                                                                               aaCCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGTGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCt                                                                   <  2:20453/149‑1 (MQ=255)
                                                                               aaCCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCttt                                                                 >  1:1053330/1‑151 (MQ=255)
                                                                               aaCCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCttt                                                                 <  2:163834/151‑1 (MQ=255)
                                                                                 ccTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTa                                                               <  4:1823562/151‑1 (MQ=255)
                                                                                 ccTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTa                                                               <  4:1915089/151‑1 (MQ=255)
                                                                                   tGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTAt                                                              >  4:1080351/1‑150 (MQ=255)
                                                                                    gCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCt                                                            >  4:1743180/1‑151 (MQ=255)
                                                                                     cAATGACGAGAACTTCACCACCAAGGCGGGTGCCCCGCGGGTAGCGGCGGAAATGGGGATTTCGCCGGTGATGCCAGACACCAGCCCGCGCGGGGAAAAGGTTGCCCAC‑GACGATGGCTTAAAATTAGGCCAGGGCGGAGGCCTTTTTCtt                                                           >  3:2276/1‑151 (MQ=255)
                                                                                       aTGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGGGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTa                                                          >  4:298615/1‑150 (MQ=255)
                                                                                         gACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTaa                                                         >  4:1409527/1‑149 (MQ=255)
                                                                                         gACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAAt                                                        <  1:1376085/150‑1 (MQ=255)
                                                                                         gACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATg                                                       <  4:1654457/151‑1 (MQ=255)
                                                                                             agaACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCAc                                                   >  1:186229/1‑151 (MQ=255)
                                                                                              gaACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACg                                                  <  4:1837280/151‑1 (MQ=255)
                                                                                                aCTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCa                                                <  4:1404741/151‑1 (MQ=255)
                                                                                                aCTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCa                                                <  2:161443/151‑1 (MQ=255)
                                                                                                   tCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCaa                                               <  1:438868/149‑1 (MQ=255)
                                                                                                      ccaccaAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAAccgcc                                          <  4:748335/151‑1 (MQ=255)
                                                                                                           aggggggggccccAGCGGGTGGGGGCGGAACTGGGGATTGTGCGGGGGATGCCAGACACCATCCCGCGGGGCGAAAAGGTTGCCAAC‑GACGATGCCTGCATTTGAGGCCGGGGCCCAGGTTTTTTTCTAAATCCCCCGCAACCGCCGTgg                                      <  1:1239473/140‑1 (MQ=255)
                                                                                                           aaGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTggg                                     >  4:1749492/1‑151 (MQ=255)
                                                                                                                   tGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCAt                             >  1:1420390/1‑151 (MQ=255)
                                                                                                                     cccGGCGGGTGGCGGCGGAACTGGGGATTGTGCGGGTAATGCCAGACCCCACCCCGCGCGGAGAAAAGGTTGCCAAC‑GACGAGGGCTACGATTTAGGCGGGGGCGCAGGTTTTTATCTTAAGCCCACGCAGCCGCCGTGGGCGACGCATTa                           <  1:390603/151‑1 (MQ=255)
                                                                                                                     cccAGCGGGTGGCGGCGGAACGGGGGATTGTGCTGGTGATGCCAGACCCCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATCCCACGCAACCGCCGTGGGCGACGCAt                             <  2:284626/149‑1 (MQ=255)
                                                                                                                       cAGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTAcc                         >  4:1289176/1‑151 (MQ=255)
                                                                                                                        aGCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCg                        >  1:345887/1‑151 (MQ=255)
                                                                                                                         gggggTAGCGGCGGAACGGGGGATGGTGCGGGTGAGGCCAGACACCAGCCCGCGCGGCGAAAGGGTTGCCAAC‑GACGATGGCTACGTTTTAGGCCGGGGCGCAGGCTTTTATTTAAAGGCCACGCAACCGCCGTGGGCGACGCATTAcc                         <  1:1046945/147‑1 (MQ=255)
                                                                                                                         gCGGGTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCgg                       >  4:64591/1‑151 (MQ=255)
                                                                                                                          tgggTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCgg                       >  1:1504832/2‑150 (MQ=255)
                                                                                                                           gggTGGCGGCGGAACTGGGGATTGTGCGGGTGATCCCAGACCCCAGCCCGCGCGGAGAAAAGGTGCCCAAC‑GACGATGGCTCCGATTTAGGCCGGGGCGCAGGTTTTTCTCTTACTCCCCCGCAACCGCCGTGGGCGACGCATTACCg                        <  1:780130/148‑1 (MQ=255)
                                                                                                                           gggTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGa                      >  1:921888/1‑150 (MQ=255)
                                                                                                                           gggTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGAt                     >  4:1057012/1‑151 (MQ=255)
                                                                                                                             gTAGCGGCGGAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTAAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGt                   <  2:126441/151‑1 (MQ=255)
                                                                                                                               aGCGGGGAAACGGGGGATTGTGCTGGTGATGCCACACACCAGCCCGCGCGGAAAAAAGGTGGCAAAC‑GAGGATGGCTAGGATTGAGGCCGGGGCGAGGGTTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTa                  <  1:510352/150‑1 (MQ=255)
                                                                                                                                  ggcggAACGGGGGTTTGGGCTGGTGAGGCCAGACACCGGCCCGCGGGGCGAAAGGGTGCCCACC‑GACGAGGGCTAGGATTTAGGCCGGGGCGAAGGCTTTTATTTTAAGGCCAGGCAACCGCCGTGGGCGACGCATTACCGGATGTATg                <  1:545034/149‑1 (MQ=255)
                                                                                                                                      gAACTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATc          >  1:770730/1‑151 (MQ=255)
                                                                                                                                        aCTGGGGATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTg        >  1:357728/1‑151 (MQ=255)
                                                                                                                                           ggggATTGTGCTGGTGATGCCAGACACCAGCCGGCGGGGCGAAAAGGTTGCCAAC‑GACGGTGGCTACGATTTAGGCCGGGGCGCGGGTTTTTATCTTAACGCCACGAAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgc       <  4:977530/149‑1 (MQ=255)
                                                                                                                                           ggggATTGTGCTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgcgc     >  4:1234261/1‑151 (MQ=255)
                                                                                                                                            gggTTTGTGCGGGGGAAGCAAGACCCGAGCCCGCG‑GGGAAAAGGGGTTCCAACTGAAGATGGCTAACATTAAGCCGCGGGCGAAGCTTTTTATTTAAATGCCACGAAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTgcg      <  4:199952/149‑1 (MQ=255)
                                                                                                                                            gggATGGTGCTGGTGATGCCAGACCCCAGCCGGCGCGGCGAAAAGTTGGCAAAC‑GACGATGGCTACGATTTAGGCCGGGGCGCAGGCTTTTATCTTAATGCCACGCAGCCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGcgcg    <  1:1396668/151‑1 (MQ=255)
                                                                                                                                                ttGTGCTGGGGATCCCAGACACCGCCCCGCGGGGGGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTGGGCCGGGGGGAAGGTTTTTATCTTAATCCCCCGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAt  <  4:719567/149‑1 (MQ=255)
                                                                                                                                                ttGTGCGGGTGATGCCAGACCCCAGCCCGCGCGGCAAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCGGGGCGCAGGTTTTTATTTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAt  <  1:710117/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCCCGATGACGTTCAGTATCTTTCTCCCTCCACCTCGTGATCACACTCCGCCACCAGTGCTGTACTGGCTTTCCGGATTAACCTGCAATGACGAGAACTTCACCACCAAGGCGGGTGCCCAGCGGGTAGCGGCGGAACTGGGGATTGTACTGGTGATGCCAGACACCAGCCCGCGCGGCGAAAAGGTTGCCAAC‑GACGATGGCTACGATTTAGGCCAGGGCGCAGGCTTTTATCTTAATGCCACGCAACCGCCGTGGGCGACGCATTACCGGATGTATGATTATCTGCGCGAT  >  NC_000913/2243986‑2244278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A