Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I2 R1 173 150.8 4954114 96.5% 4780720 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,254,920 G→A G89G (GGC→GGT)  rihB ← ribonucleoside hydrolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,9200GA98.5% 504.5 / ‑4.5 134G89G (GGC→GGT) rihBribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (60/71);  total (63/71)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 134 total aligned reads displayed.

GGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTG  >  NC_000913/2254773‑2255066
                                                                                                                                                   |                                                                                                                                                  
gggTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGAcc                                                                                                                                                  >  1:1241118/1‑150 (MQ=255)
     gCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCa                                                                                                                                             <  1:135644/150‑1 (MQ=255)
      cATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGt                                                                                                                                           <  1:931068/151‑1 (MQ=255)
         aCGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTcc                                                                                                                                         <  4:1579797/150‑1 (MQ=255)
            cATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGtt                                                                                                                                     <  1:39894/151‑1 (MQ=255)
               tGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTc                                                                                                                                   <  4:230958/150‑1 (MQ=255)
                  cACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCg                                                                                                                               >  3:407747/1‑151 (MQ=255)
                    ccGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGCCCATCCAGTCCGGTTTCACCGTg                                                                                                                             >  1:1232727/1‑151 (MQ=255)
                        gATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAat                                                                                                                          >  1:1456867/1‑150 (MQ=255)
                         atatTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAatat                                                                                                                        <  4:1764379/151‑1 (MQ=255)
                           atTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAAtatt                                                                                                                       >  1:1880206/1‑150 (MQ=255)
                           atTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAAtatt                                                                                                                       >  4:440209/1‑150 (MQ=255)
                           atTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAAtatt                                                                                                                       >  1:1065421/1‑150 (MQ=255)
                             ttGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAAtattat                                                                                                                     <  1:1925362/150‑1 (MQ=255)
                             ttGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATc                                                                                                                    >  4:1135424/1‑151 (MQ=255)
                              tGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCCGTCCGGTTTCACCGTGAATATTATc                                                                                                                    >  1:1571212/1‑150 (MQ=255)
                              tGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCg                                                                                                                   >  4:1365104/1‑151 (MQ=255)
                               gAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCgg                                                                                                                  >  1:1011818/1‑151 (MQ=255)
                                aaaGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGc                                                                                                                 <  4:313942/151‑1 (MQ=255)
                                  aGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCaa                                                                                                               <  1:285218/151‑1 (MQ=255)
                                    cGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACg                                                                                                             <  2:90297/151‑1 (MQ=255)
                                     ggACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACg                                                                                                             >  2:473474/1‑150 (MQ=255)
                                      gACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCgg                                                                                                                                       <  2:412309/123‑1 (MQ=255)
                                        ccAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGAttt                                                                                                         <  1:1701801/151‑1 (MQ=255)
                                           aCCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATttgtt                                                                                                      >  1:711524/1‑151 (MQ=255)
                                             cGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGa                                                                                                    <  4:239609/151‑1 (MQ=255)
                                              ggCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGAc                                                                                                   <  1:1392976/151‑1 (MQ=255)
                                               gCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGAct                                                                                                  <  4:531477/151‑2 (MQ=255)
                                                cACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGc                                                                                                 >  4:657950/1‑151 (MQ=255)
                                                cACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGc                                                                                                 <  4:1646603/151‑1 (MQ=255)
                                                cACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGc                                                                                                 >  1:616582/1‑151 (MQ=255)
                                                   cAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCAt                                                                                               >  1:50517/1‑150 (MQ=255)
                                                    agagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATaa                                                                                             <  3:202298/151‑1 (MQ=255)
                                                    agagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATaa                                                                                             <  2:454339/151‑1 (MQ=255)
                                                    agagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATa                                                                                              >  1:1553895/1‑150 (MQ=255)
                                                     gagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAAt                                                                                            <  4:465231/151‑1 (MQ=255)
                                                     gagTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAAt                                                                                            <  3:224509/151‑1 (MQ=255)
                                                       gTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATgg                                                                                          <  4:1872624/151‑1 (MQ=255)
                                                                 cATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCa                                                                                 >  1:1796023/1‑150 (MQ=255)
                                                                  aTCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATc                                                                               >  4:1830064/1‑151 (MQ=255)
                                                                    cGCTTGCCATCAGGGCATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATccc                                                                             <  3:148546/151‑1 (MQ=255)
                                                                     gCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATcccc                                                                            >  4:520875/1‑151 (MQ=255)
                                                                       ttGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGc                                                                          >  2:148485/1‑151 (MQ=255)
                                                                          ccATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCAt                                                                        >  2:257163/1‑150 (MQ=255)
                                                                              cAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAAcc                                                                   >  2:358438/1‑151 (MQ=255)
                                                                               aGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCg                                                                  <  4:1347653/151‑1 (MQ=255)
                                                                                      cAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGCATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACAtt                                                           >  1:1188713/1‑151 (MQ=255)
                                                                                      cAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACAtt                                                           >  4:1686311/1‑151 (MQ=255)
                                                                                       aaTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATGCCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTa                                                          <  1:879214/151‑1 (MQ=255)
                                                                                        aTGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTaa                                                         <  4:27965/151‑1 (MQ=255)
                                                                                          gATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAAt                                                        <  3:321845/150‑1 (MQ=255)
                                                                                           atatatTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAAtct                                                      >  1:601013/1‑151 (MQ=255)
                                                                                           atatatTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAAtct                                                      >  4:1134002/1‑151 (MQ=255)
                                                                                           atatatTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAAtc                                                       >  1:828755/1‑150 (MQ=255)
                                                                                               atTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTcc                                                    >  4:1525852/1‑149 (MQ=255)
                                                                                               atTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCa                                                   <  4:789580/150‑1 (MQ=255)
                                                                                               atTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAg                                                  <  3:152757/151‑1 (MQ=255)
                                                                                                tttCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGt                                                 >  4:713082/1‑151 (MQ=255)
                                                                                                  tCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGtt                                                <  1:1122197/150‑1 (MQ=255)
                                                                                                   cACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGttt                                               >  1:430122/1‑150 (MQ=255)
                                                                                                     ccGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTcc                                                    >  4:1743571/1‑143 (MQ=255)
                                                                                                     ccGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCa                                                   <  1:1742887/144‑1 (MQ=255)
                                                                                                     ccGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCt                                             <  1:385295/150‑1 (MQ=255)
                                                                                                     ccGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCt                                             <  1:461670/150‑1 (MQ=255)
                                                                                                     ccGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCt                                             <  1:1874931/150‑1 (MQ=255)
                                                                                                      cgcgTGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTgg                                           <  1:1522589/151‑1 (MQ=255)
                                                                                                      cGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTgg                                           >  2:295546/1‑151 (MQ=255)
                                                                                                       gCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGc                                          >  1:670853/1‑151 (MQ=255)
                                                                                                        cATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCa                                         <  3:227306/151‑1 (MQ=255)
                                                                                                         aTGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCaa                                        <  1:466545/151‑1 (MQ=255)
                                                                                                            aGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACa                                     <  1:1334433/151‑1 (MQ=255)
                                                                                                              tGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACAt                                    <  4:491021/150‑1 (MQ=255)
                                                                                                               gCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTc                                  <  2:130705/151‑1 (MQ=255)
                                                                                                                cTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTc                                  >  1:1060278/1‑150 (MQ=255)
                                                                                                                  ttCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCa                                 <  1:1135600/149‑1 (MQ=255)
                                                                                                                  ttCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCa                                 <  1:943244/149‑1 (MQ=255)
                                                                                                                  ttCTGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAg                                <  1:1896279/150‑1 (MQ=255)
                                                                                                                    cTGCCTGGCGGGTCAGCGGCTCGAATATCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAgg                               >  1:1348599/1‑149 (MQ=255)
                                                                                                                     tGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGcc                             >  4:1142347/1‑150 (MQ=255)
                                                                                                                     tGCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGcc                             >  1:81660/1‑150 (MQ=255)
                                                                                                                      gCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGc                              <  4:598198/148‑1 (MQ=255)
                                                                                                                      gCCTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGc                              <  1:4772/148‑1 (MQ=255)
                                                                                                                       ccTGGCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCatt                          <  4:104406/151‑1 (MQ=255)
                                                                                                                           gCGGGTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGTCCattaatt                      <  1:1780198/151‑1 (MQ=255)
                                                                                                                             gggTCAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCattaatta                     >  3:28151/1‑150 (MQ=255)
                                                                                                                                 cAGCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGtt                <  1:764293/151‑1 (MQ=255)
                                                                                                                                  aGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGttt               >  1:110248/1‑151 (MQ=255)
                                                                                                                                   gCGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGtttt              >  1:723399/1‑151 (MQ=255)
                                                                                                                                    cGGCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGtttt              >  4:288973/1‑150 (MQ=255)
                                                                                                                                     ggCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTAt            >  4:506004/1‑151 (MQ=255)
                                                                                                                                     ggCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTAt            >  2:28530/1‑151 (MQ=255)
                                                                                                                                     ggCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTAt            <  4:1457449/151‑1 (MQ=255)
                                                                                                                                      gCTCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATc           >  2:154597/1‑151 (MQ=255)
                                                                                                                                        tCGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCa          >  4:749883/1‑150 (MQ=255)
                                                                                                                                         cGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCaa         <  4:493068/150‑1 (MQ=255)
                                                                                                                                         cGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCaa         <  3:433478/150‑1 (MQ=255)
                                                                                                                                         cGAATACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCa          <  3:60908/149‑1 (MQ=255)
                                                                                                                                            aTACCGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGt     >  4:522808/1‑151 (MQ=255)
                                                                                                                                               ccGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCt   <  2:208265/150‑1 (MQ=255)
                                                                                                                                                cGGACCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTg  >  1:290272/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGTTGCATACGCATTGCCACCGCGATATTTGAAAGCGGACCAACCGGCACCAGAGTGATATCGCCATCGCTTGCCATCAGGGTATCAATGATATATTTCACCGCATGAGTGCTTTCTGCCTGGCGGGTCAGCGGCTCGAATACCGGGCCATCCAGTCCGGTTTCACCGTGAATATTATCGGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTG  >  NC_000913/2254773‑2255066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A