Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F50 I1 R1
|
163 |
55.0 |
1534755 |
98.6% |
1513268 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
624,671 |
A→G |
intergenic (‑161/‑214) |
fepB ← / → entC |
ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 624,671 | 0 | A | G | 100.0%
| 16.2
/ NA
| 6 | intergenic (‑161/‑214) | fepB/entC | ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (1/5); total (1/5) |
AATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTT > NC_000913/624530‑624818
|
aataaGGTTAAGGGCAAAAAGACAAATACGACAACGCCCACACTCCGCCCCCTCGCCCCTGGGGGGAGGGGGTTGGGGGGGGGGGACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAgggg < 1:42170/148‑1 (MQ=255)
ccTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCAtt > 1:326874/1‑151 (MQ=255)
ggggggggccccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAc < 2:712567/138‑1 (MQ=255)
gggggggggccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc < 1:451632/139‑1 (MQ=255)
ggggggggccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCa < 1:3202/140‑1 (MQ=255)
cACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAagag < 1:763276/149‑1 (MQ=255)
ggggGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCtttt < 2:293044/151‑1 (MQ=255)
ggggGAGGGGGCAGAACGGCGCAGGACATCACATTGAGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCCCAAGAGTTGACATAGTGCGCGTTTGCtttt < 2:279829/151‑1 (MQ=255)
|
AATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTT > NC_000913/624530‑624818
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
