Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I3 R1 168 141.0 4346284 99.6% 4328898 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 624,671 A→G intergenic (‑161/‑214) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913624,6710AG99.0% 354.2 / ‑2.9 98intergenic (‑161/‑214)fepB/entCferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (69/28);  minor base . (0/1);  total (69/29)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 101 total aligned reads displayed.

TTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTT  >  NC_000913/624525‑624818
                                                                                                                                                   |                                                                                                                                                   
ttattaATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAgg                                                                                                                                                 >  2:1150565/1‑151 (MQ=255)
      ataaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGc                                                                                                                                             >  1:630421/1‑149 (MQ=255)
       taaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGc                                                                                                                                             >  1:2163879/1‑148 (MQ=255)
       taaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGc                                                                                                                                             >  2:2071764/1‑148 (MQ=255)
        aaGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGa                                                                                                                                          >  1:696963/1‑150 (MQ=255)
         aGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAAc                                                                                                                                        >  2:1418400/1‑151 (MQ=255)
            ttAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACgg                                                                                                                                      >  1:429280/1‑150 (MQ=255)
             tAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGgcg                                                                                                                                    >  2:2149468/1‑151 (MQ=255)
             tAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGgcg                                                                                                                                    >  2:2092757/1‑151 (MQ=255)
              aaGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGgcg                                                                                                                                    >  2:1203171/1‑150 (MQ=255)
               aGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGgcgc                                                                                                                                   >  1:1168680/1‑150 (MQ=255)
                    gTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGAc                                                                                                                              >  2:421444/1‑150 (MQ=255)
                        tGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATcac                                                                                                                         >  2:64482/1‑151 (MQ=255)
                               ttCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTgcgc                                                                                                                  >  2:128337/1‑151 (MQ=255)
                                 cGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCtt                                                                                                                >  2:2042186/1‑151 (MQ=255)
                                  gACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCtt                                                                                                                >  1:264785/1‑150 (MQ=255)
                                  gACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCtt                                                                                                                >  2:1826890/1‑150 (MQ=255)
                                  gACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCtt                                                                                                                >  1:1728951/1‑150 (MQ=255)
                                   aCAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTAt                                                                                                              >  1:1329954/1‑151 (MQ=255)
                                   aCAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTAt                                                                                                              >  1:2161465/1‑151 (MQ=255)
                                       aGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCg                                                                                                           >  2:1693817/1‑150 (MQ=255)
                                          gcACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATc                                                                                                       >  1:1197350/1‑151 (MQ=255)
                                            acaATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATcc                                                                                                      >  1:2091413/1‑150 (MQ=255)
                                             caATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCAt                                                                                                    >  2:139350/1‑151 (MQ=255)
                                               aTCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATc                                                                                                   >  1:1028907/1‑150 (MQ=255)
                                                  cccccccccGGCCCTTGGGGGGAGGGGGTGGGGGTGGGGGGACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATcc                                                                                                      <  1:758356/142‑1 (MQ=255)
                                                  cGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaat                                                                                                >  1:94317/1‑150 (MQ=255)
                                                   gTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaata                                                                                               >  1:1818545/1‑150 (MQ=255)
                                                    ccccccggcgggggt‑GGGGGGGGGGTGGGGGTGGGGCCACCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCaataa                                                                                              <  2:160686/136‑1 (MQ=255)
                                                      cccTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGc                                                                                           >  2:178776/1‑151 (MQ=255)
                                                       ccTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCt                                                                                          >  1:688640/1‑151 (MQ=255)
                                                           gCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtctc                                                                                      >  1:2113665/1‑151 (MQ=255)
                                                           gCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtctc                                                                                      >  1:60758/1‑151 (MQ=255)
                                                            ccccTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTtctc                                                                                      >  2:1133805/1‑150 (MQ=255)
                                                                  t‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTc                                                                                >  1:560968/1‑150 (MQ=255)
                                                                   ggggcAGGGGTTTAGGGGGAGTCAAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCa                                                                                     <  1:2067433/144‑1 (MQ=255)
                                                                    ggggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCAt                                                                              >  1:1325925/1‑151 (MQ=255)
                                                                      ggAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTg                                                                            >  1:476282/1‑151 (MQ=255)
                                                                         gagGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCAAATCCATCAATAATGCTTCTCATTTTCATTGTa                                                                          >  2:289133/1‑150 (MQ=255)
                                                                              ttAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACcac                                                                     >  1:963063/1‑150 (MQ=255)
                                                                              ttAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACcac                                                                     >  2:1270344/1‑150 (MQ=255)
                                                                              ttAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACcac                                                                     >  1:2120544/1‑150 (MQ=255)
                                                                               tggggcgtgacccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACcaca                                                                    <  2:1675611/136‑1 (MQ=255)
                                                                               gggggggggggccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACaa                                                                   <  2:1761099/137‑1 (MQ=255)
                                                                                 gggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc                                                                 >  1:2111440/1‑151 (MQ=255)
                                                                                 gggTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc                                                                 >  2:1271952/1‑151 (MQ=255)
                                                                                  ggggggcgacaCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCa                                                                <  1:1875312/141‑1 (MQ=255)
                                                                                   gtgggccccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCa                                                                <  2:246804/140‑1 (MQ=255)
                                                                                   gggcgccccccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc                                                                 <  2:1851082/139‑1 (MQ=255)
                                                                                    tGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAAcc                                                                 >  1:1796337/1‑148 (MQ=255)
                                                                                       ggggAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGc                                                           >  1:35872/1‑151 (MQ=255)
                                                                                          gAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAAc                                                        >  1:642414/1‑151 (MQ=255)
                                                                                            ccagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGc                                                           <  1:2081006/145‑1 (MQ=255)
                                                                                            aCAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAAccc                                                      >  2:1217883/1‑151 (MQ=255)
                                                                                             cagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGAt                                                             <  1:1608313/143‑1 (MQ=255)
                                                                                             cagccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATg                                                            <  2:493900/144‑1 (MQ=255)
                                                                                              agccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCg                                                    <  2:747861/151‑1 (MQ=255)
                                                                                              agccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCg                                                    >  1:1684970/1‑151 (MQ=255)
                                                                                               gccagcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGa                                                   <  1:519941/151‑1 (MQ=255)
                                                                                                  agcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGtt                                                <  1:863435/151‑1 (MQ=255)
                                                                                                  agcACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGtt                                                >  1:653752/1‑151 (MQ=255)
                                                                                                     aCTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGc                                              >  1:25167/1‑150 (MQ=255)
                                                                                                      cTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGc                                              >  1:1312170/1‑149 (MQ=255)
                                                                                                        ggTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGc                                              >  2:1151846/1‑147 (MQ=255)
                                                                                                          tGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTg                                        >  1:920444/1‑151 (MQ=255)
                                                                                                           gCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTg                                        <  1:916716/150‑1 (MQ=255)
                                                                                                           gCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTg                                        <  2:1153546/150‑1 (MQ=255)
                                                                                                           gCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTg                                        <  1:175105/150‑1 (MQ=255)
                                                                                                           gCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGc                                       <  1:1869091/151‑1 (MQ=255)
                                                                                                             gAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGt                                     <  1:889744/151‑1 (MQ=255)
                                                                                                              aaCATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGtt                                    <  1:1514338/151‑1 (MQ=255)
                                                                                                                cATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTAc                                  <  2:1953579/151‑1 (MQ=255)
                                                                                                                 aTTAACCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTAcc                                 >  1:1328586/1‑151 (MQ=255)
                                                                                                                    aaCCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTc                               >  2:1001454/1‑150 (MQ=255)
                                                                                                                    aaCCCTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTc                               >  1:224954/1‑150 (MQ=255)
                                                                                                                      cccTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAag                            <  1:764488/151‑1 (MQ=255)
                                                                                                                       ccTCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCa                              >  1:1355211/1‑148 (MQ=255)
                                                                                                                         tCACCCCAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGt                         >  1:1003351/1‑151 (MQ=255)
                                                                                                                             cccAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGAc                     >  1:163896/1‑151 (MQ=255)
                                                                                                                             cccAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGAc                     >  1:1818408/1‑151 (MQ=255)
                                                                                                                              ccAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGAc                     >  2:1319184/1‑150 (MQ=255)
                                                                                                                              ccAGCCCTCACCCTGGAAGGG‑GAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACAt                   <  1:550750/151‑1 (MQ=255)
                                                                                                                               cAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACAt                   <  2:1706766/151‑1 (MQ=255)
                                                                                                                               cAGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACAt                   >  2:404834/1‑151 (MQ=255)
                                                                                                                                aGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACAt                   >  1:1796509/1‑150 (MQ=255)
                                                                                                                                aGCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACAt                   >  1:61562/1‑150 (MQ=255)
                                                                                                                                 gCCCTCACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAg                 <  1:1157500/151‑1 (MQ=255)
                                                                                                                                      cACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTgcgc            <  2:369674/151‑1 (MQ=255)
                                                                                                                                      cACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTgcgc            <  2:52457/151‑1 (MQ=255)
                                                                                                                                      cACCCTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTgcgc            >  1:840151/1‑151 (MQ=255)
                                                                                                                                          cTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGttt        >  2:696583/1‑151 (MQ=255)
                                                                                                                                          cTGGAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGttt        >  1:1362374/1‑151 (MQ=255)
                                                                                                                                            ggAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGttt        <  2:2161419/149‑1 (MQ=255)
                                                                                                                                            ggAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTg       >  1:1260703/1‑150 (MQ=255)
                                                                                                                                            ggAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGc      >  2:600181/1‑151 (MQ=255)
                                                                                                                                             gAAGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCt     >  2:2085763/1‑151 (MQ=255)
                                                                                                                                              aaGGGGGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCtt    <  1:825659/151‑1 (MQ=255)
                                                                                                                                               agggggagggggCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCttt   <  2:410393/151‑1 (MQ=255)
                                                                                                                                                ggggGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCtttt  <  1:1832987/151‑1 (MQ=255)
                                                                                                                                                ggggGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCtttt  >  1:578670/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTATTAATAAGGTTAAGGGCGTAATGACAAATTCGACAAAGCGCACAATCCGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGTGAGGGGAACAGCCAGCACTGGTGCGAACATTAACCCTCACCCCAGCCCTCACCCTGGAAGGGAGAGGGGGCAGAACGGCGCAGGACATCACATTGCGCTTATGCGAATCCATCAATAATGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTT  >  NC_000913/624525‑624818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A