breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 24,819 A→G D810G (GAC→GGC)  ileS → isoleucyl‑tRNA synthetase
RA 33,755 T→C V980A (GTG→GCG)  carB → carbamoyl‑phosphate synthase large subunit
RA 34,120 (T)9→8 intergenic (+82/‑180) carB → / → caiF carbamoyl‑phosphate synthase large subunit/cai operon transcriptional activator
RA 39,079 C→T A13T (GCC→ACC)  caiB ← crotonobetainyl CoA:carnitine CoA transferase
RA 51,996 C→T V145V (GTG→GTA rsmA ← 16S rRNA m(6)A1518, m(6)A1519 dimethyltransferase, SAM‑dependent
RA 124,759 A→G A581A (GCA→GCG aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
RA 158,411 T→C N239S (AAC→AGC)  pcnB ← poly(A) polymerase
RA 158,903 C→T G75D (GGC→GAC)  pcnB ← poly(A) polymerase
RA 216,672 A→G V111A (GTC→GCC)  yaeF ← putative lipoprotein
RA 231,852 T→C V244A (GTA→GCA)  yafD → endo/exonuclease/phosphatase family protein
RA 250,026 A→G pseudogene (45/1713 nt) lfhA ← pseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis
MC JC 257,908 Δ776 bp [crl] [crl]
RA 267,378 A→G W210R (TGG→CGG)  yafZ ← CP4‑6 prophage; conserved protein
RA 268,643 C→T A106A (GCG→GCA ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 276,808 T→C C32R (TGT→CGT)  mmuM → CP4‑6 prophage; S‑methylmethionine:homocysteine methyltransferase
RA 282,361 C→T G28G (GGC→GGT yagE → 2‑keto‑3‑deoxy gluconate (KDG) aldolase; CP4‑6 prophage
RA 304,787 G→A P140L (CCG→CTG)  ecpE ← ECP production pilus chaperone
RA 356,227 Δ1 bp coding (57/1284 nt) codA → cytosine/isoguanine deaminase
RA 380,022 (G)10→11 intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein
RA 384,316 C→G A101P (GCC→CCC)  yaiS ← putative PIG‑L family deacetylase
RA 393,298 T→C pseudogene (329/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 409,043 T→C intergenic (+93/+65) ykiA → / ← rdgC pseudogene/nucleoid‑associated ssDNA and dsDNA binding protein; competitive inhibitor of RecA function
RA 411,433 A→G L350P (CTG→CCG)  araJ ← L‑arabinose‑inducible putative transporter, MFS family
RA 415,168 C→T A196T (GCC→ACC)  sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 447,954 A→G I31I (ATT→ATC cyoD ← cytochrome o ubiquinol oxidase subunit IV
RA 448,605 T→C H19R (CAC→CGC)  cyoC ← cytochrome o ubiquinol oxidase subunit III
RA 459,446 T→C S187P (TCC→CCC)  lon → DNA‑binding ATP‑dependent protease La
RA 471,711 A→G N359S (AAC→AGC)  mdlB → putative multidrug ABC transporter ATPase
RA 547,005 A→G D109G (GAC→GGC)  fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 551,295 C→T T286M (ACG→ATG)  ybcF → putative carbonate kinase
RA 562,582 A→G T83A (ACT→GCT)  sfmH → FimA homolog, function unknown
RA 602,667 G→A D237N (GAT→AAT)  pheP → phenylalanine transporter
RA 624,671 A→G intergenic (‑161/‑214) fepB ← / → entC ferrienterobactin ABC transporter periplasmic binding protein/isochorismate synthase 1
RA 641,704 A→G V55A (GTG→GCG)  uspG ← universal stress protein UP12
RA 642,942 C→T G285G (GGC→GGT ybdR → putative Zn‑dependent NAD(P)‑binding oxidoreductase
RA 648,555 A→G R339R (CGT→CGC citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
RA 649,250 C→T V108I (GTA→ATA)  citF ← citrate lyase, citrate‑ACP transferase (alpha) subunit
RA 649,679 A→G V271A (GTG→GCG)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 671,974 G→A D71D (GAC→GAT lptE ← LPS assembly OM complex LptDE, lipoprotein component
RA 748,804 T→C D60G (GAC→GGC)  ybgO ← putative fimbrial protein
RA 789,246 A→G V245A (GTC→GCC)  galK ← galactokinase
RA 914,373 A→G V162A (GTC→GCC)  ybjE ← putative transporter
JC JC 944,912 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp intergenic (+16/+14) dmsC → / ← ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
RA 953,431 A→G S42P (TCC→CCC)  pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 1,022,355 A→G L510S (TTA→TCA)  yccS ← putative transporter, FUSC superfamily inner membrane protein
RA 1,038,253 A→G S172G (AGC→GGC)  cbdA → cytochrome bd‑II oxidase, subunit I
RA 1,070,398 A→G R186R (CGT→CGC rutE ← putative malonic semialdehyde reductase
RA 1,091,158 T→C T378A (ACC→GCC)  pgaA ← biofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein
RA 1,097,218 (G)7→8 intergenic (+389/+347) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,109,893 C→T R187C (CGC→TGC)  opgG → OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase
RA 1,124,108 (G)6→7 intergenic (‑54/+10) grxB ← / ← mdtH glutaredoxin 2 (Grx2)/multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
RA 1,142,200 C→T R723H (CGT→CAT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,232,757 T→C T92A (ACC→GCC)  dsbB ← oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
RA 1,257,682 A→G V44A (GTA→GCA)  ychF ← catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
RA 1,274,435 A→G T218A (ACA→GCA)  ychO → putative invasin
RA 1,307,511 A→G W379R (TGG→CGG)  clsA ← cardiolipin synthase 1
RA 1,356,140 T→C N324S (AAC→AGC)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein
JC JC 1,393,457 IS186 (–) +5 bp coding (231‑235/1614 nt) mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,400,721 A→G F351F (TTT→TTC abgT ← p‑aminobenzoyl‑glutamate transporter; membrane protein
RA 1,405,951 C→T intergenic (+302/‑28) abgR → / → smrA putative DNA‑binding transcriptional regulator of abgABT operon/DNA endonuclease
RA 1,429,684 A→G S212S (TCA→TCG stfR → Rac prophage; putative tail fiber protein
RA 1,496,196 A→G R303R (CGA→CGG ydcJ → putative metalloenzyme
RA 1,568,898 A→G intergenic (‑75/+56) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
RA 1,636,093 G→A pseudogene (275/528 nt) nohQ ← pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin
RA 1,670,120 T→C V141A (GTG→GCG)  ynfM → putative arabinose efflux transporter
RA 1,686,077 A→G L171P (CTG→CCG)  fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,696,856 T→C D66G (GAC→GGC)  uidR ← transcriptional repressor
RA 1,756,709 G→A V338M (GTG→ATG)  pykF → pyruvate kinase I
RA 1,768,945 A→G intergenic (‑260/‑129) ydiJ ← / → ydiK putative FAD‑linked oxidoreductase/UPF0118 family inner membrane protein
RA 1,787,381 A→G intergenic (‑269/‑64) ppsA ← / → ppsR phosphoenolpyruvate synthase/PEP synthase kinase and PEP synthase pyrophosphorylase
RA 1,811,829 T→C V299A (GTG→GCG)  ydjN → putative transporter
RA 1,840,491 A→G H342R (CAC→CGC)  ynjE → molybdopterin synthase sulfurtransferase
RA 1,931,374 A→G Q165R (CAG→CGG)  purT → phosphoribosylglycinamide formyltransferase 2
RA 1,941,604 G→A A11V (GCG→GTG)  mepM ← murein DD‑endopeptidase, space‑maker hydrolase, septation protein
RA 1,951,184 G→A R118H (CGC→CAC)  yecD → isochorismatase family protein
RA 1,969,428 T→C A519A (GCA→GCG tap ← methyl‑accepting protein IV
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,032,972 C→T T36T (ACG→ACA yedJ ← putative HD superfamily phosphohydrolase
RA 2,042,112 T→C intergenic (+87/‑256) zinT → / → yodB zinc and cadmium binding protein, periplasmic/cytochrome b561 homolog
RA 2,049,656 T→C A1573A (GCT→GCC yeeJ → putative adhesin
RA 2,074,695 A→G intergenic (+37/‑84) flu → / → yeeR CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
RA 2,088,853 T→C V92V (GTA→GTG yeeZ ← putative epimerase
RA 2,130,545 G→A R112C (CGC→TGC)  wcaD ← putative colanic acid polymerase
RA 2,150,746 T→C G80G (GGA→GGG yegI ← protein kinase‑related putative non‑specific DNA‑binding protein
RA 2,168,019 G→A V96V (GTC→GTT yegR ← uncharacterized protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,178,383 C→T R61H (CGT→CAT)  fbaB ← fructose‑bisphosphate aldolase class I
RA 2,197,150 T→C pseudogene (677/2847 nt) yehH → DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
RA 2,209,024 T→C L353P (CTG→CCG)  yehP → VMA domain putative YehL ATPase stimulator
RA 2,221,366 T→C K208K (AAA→AAG bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,224,823 A→G A16A (GCT→GCC pbpG ← D‑alanyl‑D‑alanine endopeptidase
RA 2,232,230 T→C C129C (TGT→TGC cdd → cytidine/deoxycytidine deaminase
RA 2,306,693 G→A A21V (GCG→GTG)  mqo ← malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,346,802 G→A A646A (GCG→GCA nrdA → ribonucleoside‑diphosphate reductase 1, alpha subunit
RA 2,366,486 G→A A187T (GCT→ACT)  arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
RA 2,376,592 A→G G76G (GGT→GGC menB ← dihydroxynaphthoic acid synthetase
RA 2,380,055 T→C T197A (ACT→GCT)  menF ← isochorismate synthase 2
RA 2,386,502 T→C S215P (TCC→CCC)  yfbL → putative M28A family peptidase
RA 2,438,860 A→G D304G (GAC→GGC)  flk → putative flagella assembly protein
RA 2,448,157 T→C N95S (AAC→AGC)  prmB ← N5‑glutamine methyltransferase
RA 2,474,212 A→G T61A (ACT→GCT)  yfdQ → CPS‑53 (KpLE1) prophage; uncharacterized protein
RA 2,503,689 A→G I266T (ATT→ACT)  fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,511,901 A→G V269A (GTT→GCT)  mntH ← manganese/divalent cation transporter
RA 2,559,936 T→C V360A (GTC→GCC)  intZ → CPZ‑55 prophage; putative phage integrase
RA 2,704,016 A→G T16T (ACT→ACC rnc ← RNase III
RA 2,715,479 G→A T19T (ACG→ACA eamB → cysteine and O‑acetylserine exporter
RA 2,720,149 T→C L66S (TTG→TCG)  pka → protein lysine acetyltransferase
RA 2,762,176 C→T V455V (GTG→GTA yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
RA 2,786,205 T→C intergenic (‑368/‑192) ileY ← / → ygaQ tRNA‑Ile/uncharacterized protein; putative enzyme
RA 2,794,796 G→A E182K (GAA→AAA)  gabP → gamma‑aminobutyrate transporter
RA 2,799,269 A→G M36V (ATG→GTG)  alaE → alanine exporter, alanine‑inducible, stress‑responsive
RA 2,809,774 (T)7→6 coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 2,894,005 G→A R29R (CGG→CGA ygcO → putative 4Fe‑4S cluster‑containing protein
RA 2,912,885 A→G I256T (ATC→ACC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,935,849 G→A C89Y (TGC→TAC)  fucI → L‑fucose isomerase
RA 3,005,111 A→G S226P (TCA→CCA)  ygeV ← putative sigma‑54‑interacting transcriptional activator
RA 3,009,378 A→G E205E (GAA→GAG ygeY → putative peptidase
RA 3,061,828 G→A E327K (GAA→AAA)  scpA → methylmalonyl‑CoA mutase
RA 3,062,799 G→A T650T (ACG→ACA scpA → methylmalonyl‑CoA mutase
RA 3,101,039 (C)7→8 coding (585/720 nt) yggN ← DUF2884 family putative periplasmic protein
RA 3,157,770 A→G T41A (ACT→GCT)  yqhG → DUF3828 family putative periplasmic protein
RA 3,264,789 C→T G77S (GGC→AGC)  tdcC ← L‑threonine/L‑serine transporter
RA 3,280,131 A→G E406E (GAA→GAG kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit
RA 3,335,436 A→G I353I (ATT→ATC murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,339,346 T→C V240V (GTA→GTG mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
RA 3,343,858 T→C V160A (GTA→GCA)  lptA → lipopolysaccharide export ABC transporter periplasmic binding protein; Lipid A binding protein; LPS export and assembly protein
RA 3,373,078 T→C T167A (ACC→GCC)  nanA ← N‑acetylneuraminate lyase
RA 3,376,927 A→G L165P (CTG→CCG)  sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
RA 3,386,861 A→G L443P (CTG→CCG)  aaeB ← p‑hydroxybenzoic acid efflux system component
RA 3,423,401 A→G intergenic (+207/+22) yhdZ → / ← rrfF putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
RA 3,431,635 G→A R110W (CGG→TGG)  yrdD ← ssDNA‑binding protein, function unknown
RA 3,438,469 (A)6→5 coding (181/219 nt) arfA ← alternate ribosome‑rescue factor A
RA 3,505,488 A→G V178A (GTG→GCG)  yhfS ← FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,575,211 T→C A47A (GCT→GCC yhgN → UPF0056 family inner membrane protein
RA 3,592,873 T→C T189A (ACT→GCT)  livF ← branched‑chain amino acid ABC transporter ATPase
RA 3,619,262 C→T A24V (GCC→GTC)  rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 3,622,455 T→C S1088S (AGT→AGC rhsB → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 3,623,539 T→C G47G (GGT→GGC yhhH → putative NTF2 fold immunity protein for polymorphic toxin RhsB
RA 3,645,539 G→A S52N (AGC→AAC)  rlmJ → 23S rRNA m(6)A2030 methyltransferase, SAM‑dependent
RA 3,783,056 C→T A202A (GCG→GCA gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)
RA 3,811,334 A→G R28R (CGT→CGC rpmG ← 50S ribosomal subunit protein L33
RA 3,815,808 (A)8→7 intergenic (‑40/+26) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,816,407 G→A pseudogene (143/669 nt) rph ← ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,819,035 T→C Y54Y (TAT→TAC yicG → UPF0126 family inner membrane protein
RA 3,840,839 A→G W301R (TGG→CGG)  nepI ← putative transporter
RA 3,856,193 G→A L39F (CTT→TTT)  yidH ← DUF202 family inner membrane protein
RA 3,899,764 C→T G272S (GGC→AGC)  yieL ← putative xylanase
RA 3,929,378 A→G C76R (TGC→CGC)  viaA ← stimulator of RavA ATPase activity; von Willebrand factor domain protein
RA 3,931,561 (G)7→8 coding (246/1869 nt) kup → potassium transporter
RA 4,040,701 A→G intergenic (+65/+205) rrfA → / ← mobB 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,128,197 T→C T67A (ACC→GCC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding
RA 4,150,087 A→G V60A (GTA→GCA)  eptC ← LPS heptose I phosphoethanolamine transferase
RA 4,150,739 A→G V787A (GTA→GCA)  ppc ← phosphoenolpyruvate carboxylase
RA 4,223,586 C→T V15I (GTT→ATT)  iclR ← transcriptional repressor
RA 4,254,807 C→T D554N (GAT→AAT)  plsB ← glycerol‑3‑phosphate O‑acyltransferase
RA 4,270,398 A→G N54S (AAC→AGC)  yjbQ → thiamine phosphate synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,337,788 A→G S48P (TCC→CCC)  adiY ← adi system transcriptional activator
RA 4,390,985 C→T E175E (GAG→GAA rsgA ← ribosome small subunit‑dependent GTPase A
RA 4,399,151 Δ1 bp coding (1740/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,408,885 (A)6→5 coding (2232/2442 nt) rnr → exoribonuclease R, RNase R
RA 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
RA 4,431,878 T→C T36A (ACG→GCG)  ytfE ← iron‑sulfur cluster repair protein RIC
RA 4,503,797 A→G intergenic (‑366/‑261) insG ← / → yjhB IS4 transposase/putative MFS transporter, membrane protein
RA 4,529,852 A→G V131A (GTG→GCG)  sgcC ← putative PTS system EIIC permease component
RA 4,550,829 C→T L156L (CTG→CTA gntP ← fructuronate transporter
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423703–3424234 3424583–3424238 5–881 89 [84] [84] 86 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3944107–3946170 3946170 1–2064 87 [85] [85] 87 rrlC 23S ribosomal RNA of rrnC operon

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)125 (0.920) 108/290 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein