Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	914,373	A→G	V162A (GTC→GCC)	ybjE ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	914,373	0	A	G	100.0%	161.9 / NA	42	V162A (GTC→GCC)	ybjE	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/25); total (17/25)

ACCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGACAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGC  >  NC_000913/914228‑914520

aCCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCa                                                                                                                                                   >  2:86490/1‑148 (MQ=255)

aCCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCa                                                                                                                                                   <  1:86491/148‑1 (MQ=255)

   ggAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGc                                                                                                                                             <  2:613826/151‑1 (MQ=255)

    gAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCt                                                                                                                                            <  1:345515/151‑1 (MQ=255)

     aaaGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCtt                                                                                                                                           <  1:701218/151‑1 (MQ=255)

     aaaGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCt                                                                                                                                            <  2:766608/150‑1 (MQ=255)

            aTACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTc                                                                                                                                    <  2:765540/151‑1 (MQ=255)

               ccAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATg                                                                                                                                 >  1:463072/1‑151 (MQ=255)

                           ggAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTgcgc                                                                                                                     >  1:134854/1‑151 (MQ=255)

                             aGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCaa                                                                                                                   <  2:48332/151‑1 (MQ=255)

                                 cATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGa                                                                                                               <  1:755989/151‑1 (MQ=255)

                                     gCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTTCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATAc                                                                                                           >  1:115959/1‑151 (MQ=255)

                                       cAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCa                                                                                                         <  2:291664/151‑1 (MQ=255)

                                           gcgGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGa                                                                                                     <  2:662526/151‑1 (MQ=255)

                                            cgGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACg                                                                                                      >  1:618866/1‑149 (MQ=255)

                                                                 gAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACgt                                                                               >  2:460052/1‑151 (MQ=255)

                                                                  aaTAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACgtg                                                                              <  1:296928/151‑1 (MQ=255)

                                                                     aaaGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTAt                                                                           <  1:42738/151‑1 (MQ=255)

                                                                       aGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTc                                                                         <  1:440083/151‑1 (MQ=255)

                                                                            ttaattaaACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTgg                                                                    >  1:16533/1‑151 (MQ=255)

                                                                               attaaACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCtt                                                                 <  1:134350/151‑1 (MQ=255)

                                                                                  aaaCCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTc                                                                <  2:170600/149‑1 (MQ=255)

                                                                                  aaaCCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCgg                                                              <  1:166553/151‑1 (MQ=255)

                                                                                        ccaATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCgt                                                        <  1:608985/151‑1 (MQ=255)

                                                                                         caATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCgtg                                                       >  1:154689/1‑151 (MQ=255)

                                                                                           aTTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGtgtt                                                     >  1:406518/1‑151 (MQ=255)

                                                                                                   aCTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGaaa                                             <  2:424880/151‑1 (MQ=255)

                                                                                                       aCAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCa                                         <  2:480051/151‑1 (MQ=255)

                                                                                                         aaCCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGt                                       >  2:136334/1‑151 (MQ=255)

                                                                                                          accaccaccACGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTc                                      <  2:126263/151‑1 (MQ=255)

                                                                                                            caccaccacGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTcc                                     >  1:89719/1‑150 (MQ=255)

                                                                                                             accaccacGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCAc                                   >  1:729858/1‑151 (MQ=255)

                                                                                                                 ccacGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAg                               <  1:249599/151‑1 (MQ=255)

                                                                                                                    cGGCGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGAcc                            <  1:288056/151‑1 (MQ=255)

                                                                                                                       cGACAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAAt                         >  1:43979/1‑151 (MQ=255)

                                                                                                                         aCAATCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATgg                       >  2:36223/1‑151 (MQ=255)

                                                                                                                           aaTCATTCCCCGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCa                     <  2:167735/151‑1 (MQ=255)

                                                                                                                                  ccccGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCa               <  1:108269/150‑1 (MQ=255)

                                                                                                                                  ccccGGCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCa               <  2:133215/150‑1 (MQ=255)

                                                                                                                                      ggCGATTAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCac          >  1:753201/1‑151 (MQ=255)

                                                                                                                                           ttAAGGGCAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCactact      >  1:222228/1‑150 (MQ=255)

                                                                                                                                              aGGGCAATCTGCTTTAAGGACATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGCAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTAcgc  >  1:54841/1‑151 (MQ=255)

ACCGGAAAGAGAATACCAGCCGAAACCGGAGGCCATTGCCAGCGCGGTATTGATGGGGAGATCAAGAATAAAGGCGTTAATTAAACCACCAATTAATGAACTGACAACCACCACCACGGCGACAATCATTCCCCGGCGATTAAGGACAATCTGCTTTAAGGTCATGCCATTATTGCGCAACTGAATACCAACGAGGAAAAGTAGCAAAATTAACGTGTATTCACTGGCTTCGGTCGCGTGTTGTAAGAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGC  >  NC_000913/914228‑914520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A