Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	953,431	A→G	S42P (TCC→CCC)	pflB ←	formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,431	0	A	G	100.0%	150.7 / NA	43	S42P (TCC→CCC)	pflB	formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/21); total (22/21)

TTGATGTAGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGT  >  NC_000913/953283‑953575

ttgATGTAGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCt                                                                                                                                                >  1:4842/1‑151 (MQ=255)

       aGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCTCCAGCCAGTAAGGGCTCGTCAcc                                                                                                                                         <  2:449668/151‑1 (MQ=255)

         ccAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCt                                                                                                                                       >  2:194761/1‑151 (MQ=255)

          cAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTc                                                                                                                                      <  2:339152/151‑1 (MQ=255)

            tcgtcgtGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGt                                                                                                                                    >  1:395580/2‑151 (MQ=255)

                          gATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCGGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGttt                                                                                                                      >  2:632503/1‑151 (MQ=255)

                             ggtggAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGttttt                                                                                                                    <  1:297253/150‑1 (MQ=255)

                               tggAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCtg                                                                                                                 >  2:442601/1‑151 (MQ=255)

                                      aaCAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCtgaatga                                                                                                            >  2:513708/1‑149 (MQ=255)

                                         aGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCa                                                                                                       <  1:562359/151‑1 (MQ=255)

                                          gCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCAc                                                                                                      >  2:734946/1‑151 (MQ=255)

                                            ggTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACgg                                                                                                    >  2:330058/1‑151 (MQ=255)

                                             gtgtCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACgg                                                                                                    >  1:406629/1‑150 (MQ=255)

                                                     gTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACt                                                                                           <  2:571150/151‑1 (MQ=255)

                                                       cAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTc                                                                                         <  1:596770/151‑1 (MQ=255)

                                                         aCTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCAt                                                                                       <  2:427810/151‑1 (MQ=255)

                                                          cTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCAtt                                                                                      >  2:592782/1‑151 (MQ=255)

                                                          cTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCAtt                                                                                      >  1:359891/1‑151 (MQ=255)

                                                                 gTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAg                                                                               <  1:761447/151‑1 (MQ=255)

                                                                  tgtgTGCGGATTTCCAGGTTAACGCCGTCCATTACTTTGTCCCACAGGGTGGAGTTCGCTTCAGTAGCGCCAGCCAGGAAGTGCTGATCACCCTCGTACGGAGTGTATTTTTCCTGACTAAAGTCACGAACGTTTTCTTCATTCTTCCAGt                                                                              >  2:420614/4‑151 (MQ=255)

                                                                       gCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCAcc                                                                          <  1:451990/150‑1 (MQ=255)

                                                                         ggTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCttt                                                                       >  2:443065/1‑151 (MQ=255)

                                                                          gTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTg                                                                      <  2:646663/151‑1 (MQ=255)

                                                                                   tttAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCt                                                                                    >  2:322157/1‑128 (MQ=255)

                                                                                   tttAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCt                                                                                    <  1:322170/128‑1 (MQ=255)

                                                                                    ttAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCtt                                                            >  1:230212/1‑151 (MQ=255)

                                                                                         gCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTccc                                                         >  1:629140/1‑149 (MQ=255)

                                                                                                aTTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTgg                                                <  1:51440/151‑1 (MQ=255)

                                                                                                       tGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACtt                                         >  1:45142/1‑151 (MQ=255)

                                                                                                        gTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACttt                                        >  2:662383/1‑151 (MQ=255)

                                                                                                           ccACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTc                                      >  2:56810/1‑150 (MQ=255)

                                                                                                             acaGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCAtt                                   >  2:34009/1‑151 (MQ=255)

                                                                                                              caGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCAtt                                   >  1:481085/1‑150 (MQ=255)

                                                                                                               aGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTaa                                 <  1:330071/151‑1 (MQ=255)

                                                                                                                    ggtggtCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCg                            <  2:424453/151‑1 (MQ=255)

                                                                                                                       ggtCGCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTCTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGa                          <  2:478592/150‑1 (MQ=255)

                                                                                                                           gCTTCAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGt                     <  2:239794/151‑1 (MQ=255)

                                                                                                                               cAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTaa                   <  2:684935/149‑1 (MQ=255)

                                                                                                                               cAGTAGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAac                  <  2:580800/150‑1 (MQ=255)

                                                                                                                                   aGCGCCAGCCAGGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCGTTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACaccta             <  2:370605/151‑1 (MQ=255)

                                                                                                                                         agccagGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACcttct       >  1:321343/1‑151 (MQ=255)

                                                                                                                                          gccagGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACcttctt      <  2:321330/151‑1 (MQ=255)

                                                                                                                                            cagGAAGGGCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTaa    <  1:330685/151‑1 (MQ=255)

                                                                                                                                              ggaagggCTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGt  <  1:497013/151‑1 (MQ=255)

TTGATGTAGCCAGCGTCGTGAGAGGTGATGGTGGAAGCAACAGCGGTGTCAAAGTCAACTGGCGCGTGAGTGCGGTTTTCCAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGT  >  NC_000913/953283‑953575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A