Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F50 I1 R1
|
163 |
55.0 |
1534755 |
98.6% |
1513268 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,198,749 |
A→G |
I19V (ATT→GTT) |
lit → |
T4 phage exclusion protein; cell death peptidase, e14 prophage |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,198,749 | 0 | A | G | 100.0%
| 22.7
/ NA
| 7 | I19V (ATT→GTT) | lit | T4 phage exclusion protein; cell death peptidase, e14 prophage |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (5/2); total (5/2) |
ACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAC > NC_000913/1198622‑1198824
|
aCATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCa > 2:562998/1‑147 (MQ=255)
aaaCTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACg > 2:165716/1‑151 (MQ=255)
aTCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTgacgac > 1:472550/1‑150 (MQ=255)
gTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATta < 1:563027/150‑1 (MQ=255)
tactacCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAAttt > 1:284936/1‑151 (MQ=255)
ttGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAc > 1:205889/1‑116 (MQ=255)
ttGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGGTTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAc < 2:205881/116‑1 (MQ=255)
|
ACATAGAAAAAACTGAAGATTCATCTTATTTTGTATATACTACCTAGCCCAACAATGTAGAGGTTAACGAAAAATGCGCTCACCAATTTGTCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAAC > NC_000913/1198622‑1198824
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
