Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,682	A→G	V44A (GTA→GCA)	ychF ←	catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,682	0	A	G	100.0%	107.2 / NA	32	V44A (GTA→GCA)	ychF	catalase inhibitor protein; ATPase, K+‑dependent, ribosome‑associated
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (7/25); total (7/25)

CTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCTT  >  NC_000913/1257546‑1257827

cTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTc                                                                                                                                     >  2:404092/1‑151 (MQ=255)

  ggTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCg                                                                                                                                    <  1:132398/150‑1 (MQ=255)

     tACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTc                                                                                                                                >  1:422649/1‑151 (MQ=255)

      aCCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCa                                                                                                                               <  2:214315/151‑1 (MQ=255)

        ccAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAAt                                                                                                                             >  1:86801/1‑151 (MQ=255)

         cAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATg                                                                                                                            <  2:16977/151‑1 (MQ=255)

               ttCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGc                                                                                                                        >  2:460150/1‑149 (MQ=255)

                tCGCCTTTCGATTCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGGGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCa                                                                                                                       <  2:223682/149‑1 (MQ=255)

                tCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCa                                                                                                                       <  1:729183/149‑1 (MQ=255)

                              gcCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCTGTTGATCCAGGCGAGGATCAGGCATTGGTGCGAGGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTGGGcc                                                                                                       <  2:48158/151‑1 (MQ=255)

                                          aCCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCg                                                                                           <  2:637044/151‑1 (MQ=255)

                                           ccGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCgg                                                                                          <  1:125175/151‑1 (MQ=255)

                                                 atatCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTgg                                                                                    <  2:418421/151‑1 (MQ=255)

                                                     cGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCa                                                                                 <  1:119397/150‑1 (MQ=255)

                                                      gACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAgc                                                                               <  1:511947/151‑1 (MQ=255)

                                                          aaTTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGt                                                                           <  2:554567/151‑1 (MQ=255)

                                                               cATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAAc                                                                      <  2:476875/151‑1 (MQ=255)

                                                                          gAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGAttt                                                           <  2:424043/151‑1 (MQ=255)

                                                                              cGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAGTACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCg                                                       <  2:10800/151‑1 (MQ=255)

                                                                                   gCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGtt                                                  <  2:612266/151‑1 (MQ=255)

                                                                                         gTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCaa                                            <  1:310736/151‑1 (MQ=255)

                                                                                         gTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCa                                             >  2:520563/1‑150 (MQ=255)

                                                                                          ttttACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCaa                                            >  1:305774/1‑150 (MQ=255)

                                                                                              aCGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACcg                                        <  1:442774/150‑1 (MQ=255)

                                                                                               cGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACcgac                                      >  1:201428/1‑151 (MQ=255)

                                                                                                   ttCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGAt                                   <  2:737990/150‑1 (MQ=255)

                                                                                                    tCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATa                                  <  2:133203/150‑1 (MQ=255)

                                                                                                           gTTGATCCAGGCGAGGATCAGGCATTGGTGCGACGCATGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGGTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATCCCGCAttt                          <  2:351998/151‑1 (MQ=255)

                                                                                                              gATCCAGGCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGa                        <  2:351614/150‑1 (MQ=255)

                                                                                                                     gCGAGGATCAGGCATTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATg                <  1:574460/151‑1 (MQ=255)

                                                                                                                                  aTTGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCt   <  1:224931/151‑1 (MQ=255)

                                                                                                                                    tGGTGCGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCtt  <  2:652208/150‑1 (MQ=255)

CTGGTTACCCAGACCTTCGCCTTTCGATGCGCCTTTTACCAGACCGGCGATATCGACAAATTCCATGGTCGTGGGAAGCGTACGCTGCGGTTTTACGATTTCAGCCAGTTGATCCAGGCGAGGATCAGGCATTGGTACGACGCCTGTGTTCGGCTCAATGGTGCAGAATGGAAAGTTGGCCGCTTCAATACCGGCTTTGGTCAGCGCGTTGAACAGGGTAGATTTCCCGACGTTGGGCAAACCGACGATACCGCATTTGAATCCCATGATTTAAATCACCTT  >  NC_000913/1257546‑1257827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A