Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,391	T→C	T419A (ACA→GCA)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,391	0	T	C	100.0%	194.1 / NA	53	T419A (ACA→GCA)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/22); total (31/22)

AGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCG  >  NC_000913/1307249‑1307538

aGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAAcc                                                                                                                                              <  1:557209/150‑1 (MQ=255)

         cGGGTATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGtt                                                                                                                                    <  1:647922/151‑1 (MQ=255)

              aTCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGcc                                                                                                                                >  1:165795/1‑150 (MQ=255)

              aTCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCa                                                                                                                               <  1:742802/151‑1 (MQ=255)

                  aGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCg                                                                                                                            >  2:543767/1‑150 (MQ=255)

                     aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCa                                                                                                                        <  2:766972/151‑1 (MQ=255)

                      gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAg                                                                                                                       <  1:565572/151‑1 (MQ=255)

                         gTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCAc                                                                                                                    <  2:350934/151‑1 (MQ=255)

                         gTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCAc                                                                                                                    <  1:22364/151‑1 (MQ=255)

                              cgcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGctct                                                                                                               >  1:291406/1‑151 (MQ=255)

                               gcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCtt                                                                                                              <  1:378430/151‑1 (MQ=255)

                                  aaaTATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGt                                                                                                           >  1:71829/1‑151 (MQ=255)

                                                 gAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCTCGCTCTTGGTATGCAGTAACCCg                                                                                              <  2:658707/149‑1 (MQ=255)

                                                 gAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCg                                                                                              <  1:35386/149‑1 (MQ=255)

                                                  aaCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCt                                                                                           >  1:362488/1‑151 (MQ=255)

                                                        ggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAAc                                                                                     >  2:590360/1‑151 (MQ=255)

                                                         gcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACt                                                                                    <  1:575669/151‑1 (MQ=255)

                                                           gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                                                                   >  1:107998/1‑150 (MQ=255)

                                                           gAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                                                                   >  1:766994/1‑150 (MQ=255)

                                                                   cACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAttt                                                                           <  2:766552/150‑1 (MQ=255)

                                                                     ccAAAACCTTTATCGTCGATTGCCAGGGCAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTa                                                                         >  2:129050/1‑150 (MQ=255)

                                                                      cAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAc                                                                       >  2:724522/1‑151 (MQ=255)

                                                                            cTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagc                                                                 >  2:230751/1‑151 (MQ=255)

                                                                               tATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagc                                                              >  2:333747/1‑151 (MQ=255)

                                                                                aTCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcc                                                             >  1:407177/1‑151 (MQ=255)

                                                                                  cgtcgATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagcca                                                            <  2:569587/150‑1 (MQ=255)

                                                                                      gATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGt                                                       <  1:553799/151‑1 (MQ=255)

                                                                                            cAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt                                                  >  1:486362/1‑150 (MQ=255)

                                                                                             aGGGTAATCTCGAACTTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa                                                 >  2:252888/1‑150 (MQ=255)

                                                                                              gggTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaaa                                               <  2:554473/151‑1 (MQ=255)

                                                                                               ggTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaa                                                >  2:415599/1‑149 (MQ=255)

                                                                                               ggTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAg                                              <  1:708014/151‑1 (MQ=255)

                                                                                                  aaTCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAAt                                           >  2:191311/1‑151 (MQ=255)

                                                                                                              tAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGcc                               >  1:280611/1‑151 (MQ=255)

                                                                                                              tAGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGcc                               >  2:755599/1‑151 (MQ=255)

                                                                                                               aGCCACAGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGccc                              >  1:586895/1‑151 (MQ=255)

                                                                                                                  cacaGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGc                           <  2:71828/151‑1 (MQ=255)

                                                                                                                     aGACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCg                         >  1:311074/1‑150 (MQ=255)

                                                                                                                      gACTACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGAc                       <  1:213669/151‑1 (MQ=255)

                                                                                                                         tACGCATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGAc                       >  2:348636/1‑148 (MQ=255)

                                                                                                                             cATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCa                     >  1:708872/1‑146 (MQ=255)

                                                                                                                             cATATCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCa                     <  2:708829/146‑1 (MQ=255)

                                                                                                                                atCAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAg             <  1:264409/151‑1 (MQ=255)

                                                                                                                                  cAAGGTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGt            <  1:597622/150‑1 (MQ=255)

                                                                                                                                     ggTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtt        >  1:130356/1‑151 (MQ=255)

                                                                                                                                     ggTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtt        >  1:733092/1‑151 (MQ=255)

                                                                                                                                      gTTAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAttt       >  2:363164/1‑151 (MQ=255)

                                                                                                                                       ttAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAtttt      >  2:374153/1‑151 (MQ=255)

                                                                                                                                        tAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAttttt     >  2:302980/1‑151 (MQ=255)

                                                                                                                                        tAACTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCAttttt     >  2:169004/1‑151 (MQ=255)

                                                                                                                                          aCTGCGCCAACCAGACTCAGTTCGCCATCGACCAGCACGCTCTTTGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGACAGCAGTTCCGTAAAGAATGCGTGGCTGGCCCAGCCGAGCAGCATTGAGTCAtatt      <  2:642454/148‑1 (MQ=255)

                                                                                                                                           cTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTcg  >  2:599981/1‑151 (MQ=255)

                                                                                                                                           cTGCGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTcg  >  2:569053/1‑151 (MQ=255)

AGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGCCCAGCCGACCAGCATCGAGTCATTTTTTCG  >  NC_000913/1307249‑1307538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A