Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,709	G→A	V338M (GTG→ATG)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,709	0	G	A	100.0%	193.6 / NA	49	V338M (GTG→ATG)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/31); total (18/31)

GCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGC  >  NC_000913/1756563‑1756852

gCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCAtgat                                                                                                                                             <  1:462931/151‑1 (MQ=255)

   cGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCAtgatga                                                                                                                                           >  1:260502/1‑150 (MQ=255)

    gACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAAc                                                                                                                                         <  1:340797/151‑1 (MQ=255)

        cgcgCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAg                                                                                                                                       <  1:257127/149‑1 (MQ=255)

           gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGtc                                                                                                                                  <  1:202229/151‑1 (MQ=255)

                        aCGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTcaacaa                                                                                                                     <  2:665685/151‑1 (MQ=255)

                           ttGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATg                                                                                                                   >  1:710268/1‑150 (MQ=255)

                                    ccATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTaa                                                                                                         <  2:102214/151‑1 (MQ=255)

                                     cATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGGATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTaa                                                                                                         >  1:696824/1‑150 (MQ=255)

                                        ccTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACt                                                                                                      <  1:272525/150‑1 (MQ=255)

                                        ccTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTg                                                                                                     <  1:361070/151‑1 (MQ=255)

                                        ccTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTg                                                                                                     >  1:676887/1‑151 (MQ=255)

                                             aCGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAt                                                                                                <  2:400152/151‑1 (MQ=255)

                                               ggTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTa                                                                                              <  2:335105/151‑1 (MQ=255)

                                                gTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAc                                                                                             <  1:729525/151‑1 (MQ=255)

                                                      aCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATGCCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGaa                                                                                         <  1:604437/149‑1 (MQ=255)

                                                      aCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCTAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGc                                                                                       <  2:185475/151‑1 (MQ=255)

                                                      aCGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGc                                                                                       <  1:712671/151‑1 (MQ=255)

                                                          aGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTa                                                                                   <  2:708954/151‑1 (MQ=255)

                                                            tGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATg                                                                                 >  1:745537/1‑151 (MQ=255)

                                                                gCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgt                                                                             >  1:150734/1‑151 (MQ=255)

                                                                gCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCg                                                                              <  2:607700/150‑1 (MQ=255)

                                                                 cTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtg                                                                            >  1:3026/1‑151 (MQ=255)

                                                                   gTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggt                                                                          <  1:748876/151‑1 (MQ=255)

                                                                      tGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGTCGTGGTGcc                                                                       >  1:704844/1‑151 (MQ=255)

                                                                       ggTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggtg                                                                         <  2:3026/148‑1 (MQ=255)

                                                                       ggTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGGGCCg                                                                      >  2:539884/1‑151 (MQ=255)

                                                                              ccGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAc                                                               <  1:144655/151‑1 (MQ=255)

                                                                               cGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAAct                                                              >  1:19544/1‑151 (MQ=255)

                                                                                  aaaaGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgct                                                           <  2:493503/151‑1 (MQ=255)

                                                                                    aaGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGa                                                         <  2:557990/151‑1 (MQ=255)

                                                                                          aaTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                                   >  2:483930/1‑151 (MQ=255)

                                                                                             aCCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTgg                                                 >  2:323417/1‑150 (MQ=255)

                                                                                               ccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATg                                              >  2:682406/1‑151 (MQ=255)

                                                                                                   tGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTcc                                          >  1:424762/1‑151 (MQ=255)

                                                                                                    ggAAGCGGTTTCTATCATGGCGACCAGCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGAGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCg                                         <  2:38231/151‑1 (MQ=255)

                                                                                                       aGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTg                                      <  1:233606/151‑1 (MQ=255)

                                                                                                       aGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTg                                      <  1:376049/151‑1 (MQ=255)

                                                                                                         cGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGAt                                    <  2:375790/151‑1 (MQ=255)

                                                                                                                   cATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCt                          >  1:658454/1‑151 (MQ=255)

                                                                                                                    aTGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTa                         <  2:713751/151‑1 (MQ=255)

                                                                                                                          aCCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTGCCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAgg                   <  2:136320/151‑1 (MQ=255)

                                                                                                                              tCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCgg               >  2:227777/1‑151 (MQ=255)

                                                                                                                                tGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTCCCGAAGCGGTATGCCGTGGTGCCGCTGAACCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCGACTCAGGGc                 <  2:282986/147‑1 (MQ=255)

                                                                                                                                   gAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAAt          >  1:411678/1‑151 (MQ=255)

                                                                                                                                     aCGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTCCTCAGGGCGGTAAATCt        <  2:390424/151‑1 (MQ=255)

                                                                                                                                      cGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTg       <  2:424741/151‑1 (MQ=255)

                                                                                                                                       gTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGc      >  2:253747/1‑151 (MQ=255)

                                                                                                                                           cgaccgCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTcgc  <  1:97293/151‑1 (MQ=255)

GCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGC  >  NC_000913/1756563‑1756852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A