Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I3 R1 168 141.0 4346284 99.6% 4328898 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,756,709 G→A V338M (GTG→ATG)  pykF → pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,756,7090GA100.0% 511.1 / NA 122V338M (GTG→ATG) pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (62/60);  total (62/60)

Only 100 of 123 total aligned reads displayed.

GCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGC  >  NC_000913/1756563‑1756854
                                                                                                                                                  |                                                                                                                                                 
gCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCAtgat                                                                                                                                               <  1:1072606/151‑1 (MQ=255)
  ccGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCatgatg                                                                                                                                              >  1:1587799/1‑150 (MQ=255)
   cGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCAtgatga                                                                                                                                             >  1:1110837/1‑150 (MQ=255)
     aCTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAAc                                                                                                                                           >  1:726695/1‑150 (MQ=255)
      cTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAAc                                                                                                                                           >  1:168670/1‑149 (MQ=255)
         gcgcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCg                                                                                                                                      >  2:2171982/1‑151 (MQ=255)
           gcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGtc                                                                                                                                    >  2:1414395/1‑151 (MQ=255)
             aGAAGCCTGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGtctc                                                                                                                                  <  2:1782787/151‑1 (MQ=255)
             aGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGtctc                                                                                                                                  <  2:1671208/151‑1 (MQ=255)
                aGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAg                                                                                                                               <  1:1320823/151‑1 (MQ=255)
                    ggTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTca                                                                                                                           <  2:1335408/151‑1 (MQ=255)
                       gACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTcaaca                                                                                                                        <  1:341051/151‑1 (MQ=255)
                          gTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAAt                                                                                                                      >  2:43449/1‑150 (MQ=255)
                          gTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATg                                                                                                                     >  2:1722698/1‑151 (MQ=255)
                           ttGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATg                                                                                                                     >  1:1658119/1‑150 (MQ=255)
                             gCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGAc                                                                                                                   >  2:602634/1‑150 (MQ=255)
                               aaaCGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAAc                                                                                                                >  2:821353/1‑151 (MQ=255)
                                aaCGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAAcc                                                                                                               >  1:1073004/1‑151 (MQ=255)
                                      aTCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAAc                                                                                                         >  2:321803/1‑151 (MQ=255)
                                       tCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACt                                                                                                        >  1:964918/1‑151 (MQ=255)
                                        ccTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACt                                                                                                        <  2:1230247/150‑1 (MQ=255)
                                        ccTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTg                                                                                                       <  1:2000709/151‑1 (MQ=255)
                                             aCGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAt                                                                                                  >  1:1909793/1‑151 (MQ=255)
                                             aCGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAt                                                                                                  <  1:1269564/151‑1 (MQ=255)
                                              cGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCAtt                                                                                                 <  1:252692/151‑1 (MQ=255)
                                               ggTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTa                                                                                                <  2:670341/151‑1 (MQ=255)
                                                gTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAc                                                                                               <  1:2039433/151‑1 (MQ=255)
                                                  aCTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAcc                                                                                              <  1:2002673/150‑1 (MQ=255)
                                                  aCTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCg                                                                                             >  1:423770/1‑151 (MQ=255)
                                                   cTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCg                                                                                             >  1:498838/1‑150 (MQ=255)
                                                   cTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCg                                                                                             >  2:1246194/1‑150 (MQ=255)
                                                     gACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGaa                                                                                           <  2:539179/150‑1 (MQ=255)
                                                     gACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGaa                                                                                           <  1:1598685/150‑1 (MQ=255)
                                                     gACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAg                                                                                          <  1:803034/151‑1 (MQ=255)
                                                         cAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCgg                                                                                       >  1:115158/1‑150 (MQ=255)
                                                             gATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATg                                                                                   <  2:2035255/150‑1 (MQ=255)
                                                                gCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgt                                                                               <  2:1507817/151‑1 (MQ=255)
                                                                  tGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtgg                                                                             >  1:385995/1‑151 (MQ=255)
                                                                   gTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggt                                                                            <  1:1079166/151‑1 (MQ=255)
                                                                    tCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtggtg                                                                           >  1:1302353/1‑151 (MQ=255)
                                                                     cTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGc                                                                          >  1:1304078/1‑151 (MQ=255)
                                                                      tGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGcc                                                                         >  1:336938/1‑151 (MQ=255)
                                                                        gTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGt                                                                       <  2:1979185/151‑1 (MQ=255)
                                                                            aTCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGaa                                                                   <  1:704743/151‑1 (MQ=255)
                                                                              ccGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGaaa                                                                  <  1:691643/150‑1 (MQ=255)
                                                                                  aaaaGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgct                                                             >  1:439644/1‑151 (MQ=255)
                                                                                   aaaGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAActgctg                                                            <  2:868882/151‑1 (MQ=255)
                                                                                     aGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGa                                                           >  2:714543/1‑150 (MQ=255)
                                                                                     aGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGa                                                           >  1:1739100/1‑150 (MQ=255)
                                                                                      ggTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaa                                                         <  2:2114380/151‑1 (MQ=255)
                                                                                      ggTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaa                                                         <  2:1358975/151‑1 (MQ=255)
                                                                                       gTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGaaa                                                         >  1:1146273/1‑150 (MQ=255)
                                                                                          aaTACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACt                                                     >  2:2017472/1‑151 (MQ=255)
                                                                                             aCCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTgg                                                   >  2:869051/1‑150 (MQ=255)
                                                                                              cccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGAt                                                 <  1:1061597/151‑1 (MQ=255)
                                                                                              cccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGAt                                                 >  2:882841/1‑151 (MQ=255)
                                                                                              cccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGAt                                                 >  2:937375/1‑151 (MQ=255)
                                                                                               ccGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATg                                                <  2:1074600/151‑1 (MQ=255)
                                                                                                cGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGc                                               <  1:1532117/151‑1 (MQ=255)
                                                                                                  cTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTc                                             >  2:1813601/1‑151 (MQ=255)
                                                                                                   tGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTcc                                            >  2:1363328/1‑151 (MQ=255)
                                                                                                    ggAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCg                                           <  2:652279/151‑1 (MQ=255)
                                                                                                       aGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTg                                        <  2:2160636/151‑1 (MQ=255)
                                                                                                        gCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGa                                       <  1:859171/151‑1 (MQ=255)
                                                                                                         cGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGAt                                      <  1:2167680/151‑1 (MQ=255)
                                                                                                         cGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGAt                                      <  2:1800803/151‑1 (MQ=255)
                                                                                                          ggTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATc                                     >  2:102557/1‑151 (MQ=255)
                                                                                                           gTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATc                                     <  2:1593122/150‑1 (MQ=255)
                                                                                                           gTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCg                                    >  1:1101424/1‑151 (MQ=255)
                                                                                                           gTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCg                                    >  2:1397810/1‑151 (MQ=255)
                                                                                                             ttCTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTg                                  >  2:1138920/1‑151 (MQ=255)
                                                                                                               cTATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGt                                >  2:1267884/1‑151 (MQ=255)
                                                                                                                tATCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGtt                               >  2:472821/1‑151 (MQ=255)
                                                                                                                 aTCATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGtt                               <  1:879482/150‑1 (MQ=255)
                                                                                                                   cATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCt                            >  2:1937022/1‑151 (MQ=255)
                                                                                                                   cATGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCt                            <  1:504390/151‑1 (MQ=255)
                                                                                                                    aTGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCt                            >  2:1333610/1‑150 (MQ=255)
                                                                                                                    aTGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTa                           <  2:1960838/151‑1 (MQ=255)
                                                                                                                    aTGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTa                           <  1:963607/151‑1 (MQ=255)
                                                                                                                     tGGCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTAc                          >  1:2034372/1‑151 (MQ=255)
                                                                                                                      ggCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACt                         >  1:639507/1‑151 (MQ=255)
                                                                                                                      ggCGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACt                         <  2:1995693/151‑1 (MQ=255)
                                                                                                                        cGACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTc                        >  1:1961260/1‑150 (MQ=255)
                                                                                                                         gACCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTc                        >  2:1881757/1‑149 (MQ=255)
                                                                                                                          aCCATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAgg                     <  2:1251268/151‑1 (MQ=255)
                                                                                                                            cATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGc                   <  2:175878/151‑1 (MQ=255)
                                                                                                                            cATCTGCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGc                   >  2:5724/1‑151 (MQ=255)
                                                                                                                                 gCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGt                <  1:1238677/149‑1 (MQ=255)
                                                                                                                                 gCGAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTa               <  2:791377/150‑1 (MQ=255)
                                                                                                                                   gAACGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTTCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAAt            >  1:926441/1‑151 (MQ=255)
                                                                                                                                     aCGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCt          >  1:315602/1‑151 (MQ=255)
                                                                                                                                      cGTACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTg         <  1:1987986/151‑1 (MQ=255)
                                                                                                                                        tACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCt       >  2:1419668/1‑151 (MQ=255)
                                                                                                                                        tACCGACCGCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCt       >  1:1149131/1‑151 (MQ=255)
                                                                                                                                         accggccgCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCt       <  2:427066/150‑1 (MQ=255)
                                                                                                                                         accgaccgCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCta      <  2:498294/151‑2 (MQ=255)
                                                                                                                                         accgaccgCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCt       <  1:1554662/150‑1 (MQ=255)
                                                                                                                                           cgaccgCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTcgc    >  1:51060/1‑151 (MQ=255)
                                                                                                                                           cgaccgCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTcgc    >  1:1958084/1‑151 (MQ=255)
                                                                                                                                             accgCATGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCgcgc  >  2:734687/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGC  >  NC_000913/1756563‑1756854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A