Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,829	T→C	V299A (GTG→GCG)	ydjN →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,829	0	T	C	100.0%	183.3 / NA	52	V299A (GTG→GCG)	ydjN	putative transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/25); total (27/25)

GCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGG  >  NC_000913/1811681‑1811976

gCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTgcgc                                                                                                                                                   >  1:197764/1‑151 (MQ=255)

    gTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGAc                                                                                                                                               <  1:221034/151‑1 (MQ=255)

                  gACATCATCAAACTGGGAAGTTTCGATGACGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTCACGTTTGCCTTTAcc                                                                                                                                  >  2:274315/1‑150 (MQ=255)

                          caAACTGGGAAGTTTCGTTGTCGCGTCCTCCCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTCCCAGCCGtt                                                                                                                           <  2:197237/149‑1 (MQ=255)

                             aCTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGt                                                                                                                      >  1:137854/1‑151 (MQ=255)

                              cTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGtg                                                                                                                     >  1:485631/1‑151 (MQ=255)

                                 ggAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGtgctg                                                                                                                  >  1:184691/1‑151 (MQ=255)

                                       ttCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCt                                                                                                             >  2:328536/1‑150 (MQ=255)

                                         cGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATc                                                                                                          <  1:163012/151‑1 (MQ=255)

                                                 cgTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGa                                                                                                   >  2:570617/1‑150 (MQ=255)

                                                  gTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAt                                                                                                 >  1:631448/1‑151 (MQ=255)

                                                       aCCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTgg                                                                                             <  1:186781/150‑1 (MQ=255)

                                                          tCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAg                                                                                          >  2:95776/1‑150 (MQ=255)

                                                              tCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGcaca                                                                                      >  2:318848/1‑150 (MQ=255)

                                                                tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTACCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACaaa                                                                                    >  2:364547/1‑150 (MQ=255)

                                                                    gATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGcgt                                                                               <  2:521856/151‑1 (MQ=255)

                                                                    gATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGcgt                                                                               >  2:604769/1‑151 (MQ=255)

                                                                      ttATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGcgtcg                                                                             >  1:399363/1‑151 (MQ=255)

                                                                        aTGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCgtcgtc                                                                           <  2:453035/151‑1 (MQ=255)

                                                                             tGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGc                                                                      >  1:463052/1‑151 (MQ=255)

                                                                               cAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGATGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGACGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACCAACGCGTCGTCTGGGCGt                                                                    >  1:653366/1‑151 (MQ=255)

                                                                                 gTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGtt                                                                   >  2:460590/1‑150 (MQ=255)

                                                                                      tGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTg                                                               <  1:758748/149‑1 (MQ=255)

                                                                                         cATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATcc                                                          <  1:529410/151‑1 (MQ=255)

                                                                                          aTTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCa                                                         <  1:281909/151‑1 (MQ=255)

                                                                                              tgctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGc                                                     >  1:489945/1‑151 (MQ=255)

                                                                                               gctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATc                                                       <  2:648215/148‑1 (MQ=255)

                                                                                                ctgGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGcc                                                    >  1:725217/1‑150 (MQ=255)

                                                                                                ctgGGCATTAATGGCGTGAGTCCGCTGAAGGACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGcc                                                    >  2:133654/1‑150 (MQ=255)

                                                                                                  gggCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAg                                                  <  2:383940/150‑1 (MQ=255)

                                                                                                    gCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGttt                                               <  2:139122/151‑1 (MQ=255)

                                                                                                     cATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTc                                              >  1:144197/1‑151 (MQ=255)

                                                                                                         aaTGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTcgcc                                           <  1:661453/150‑1 (MQ=255)

                                                                                                          aTGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTcgccgc                                         >  2:691193/1‑151 (MQ=255)

                                                                                                            ggCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCct                                       >  1:719385/1‑151 (MQ=255)

                                                                                                               gTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCtt                                    >  1:34878/1‑151 (MQ=255)

                                                                                                                tGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGTTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCtt                                    <  2:597986/150‑1 (MQ=255)

                                                                                                                 gAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTc                                  <  1:10324/151‑1 (MQ=255)

                                                                                                                     ccGCTGAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGt                               <  1:92567/150‑1 (MQ=255)

                                                                                                                          gAAGTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCaa                           <  2:625906/149‑1 (MQ=255)

                                                                                                                             gTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCAtt                      <  1:513344/151‑1 (MQ=255)

                                                                                                                             gTACTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCAtt                      >  1:470717/1‑151 (MQ=255)

                                                                                                                               aCTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTgg                    <  1:62899/151‑1 (MQ=255)

                                                                                                                               aCTTCCGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTgg                    >  1:545201/1‑151 (MQ=255)

                                                                                                                                   ccGTAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAg                <  1:647243/151‑1 (MQ=255)

                                                                                                                                      tAAGGTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAAc             >  2:587852/1‑151 (MQ=255)

                                                                                                                                         ggTATGGCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGc          <  1:523840/151‑1 (MQ=255)

                                                                                                                                          gTATGGCATGCCCCGACGTTTGGCTTTACCAGCCGTTCCAGTGCTGCTTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGACCGGCt         <  2:498531/151‑1 (MQ=255)

                                                                                                                                              ggCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCt         <  2:365118/147‑1 (MQ=255)

                                                                                                                                              ggCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgcgc     >  1:106332/1‑151 (MQ=255)

                                                                                                                                              ggCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTgcgc     <  1:570648/151‑1 (MQ=255)

                                                                                                                                               gCCTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGcc    <  1:268712/151‑1 (MQ=255)

                                                                                                                                                 cTGCGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCgg  >  1:124740/1‑151 (MQ=255)

GCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTTCGCCGCCTCTTTCGGTGCAACCATTGGTCAGAACGGCTGCGCCGG  >  NC_000913/1811681‑1811976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A