Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,243,570	A→G	V28A (GTG→GCG)	folE ←	GTP cyclohydrolase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,243,570	0	A	G	100.0%	153.7 / NA	43	V28A (GTG→GCG)	folE	GTP cyclohydrolase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/18); total (25/18)

AAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTG  >  NC_000913/2243426‑2243713

aaaaTTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGcgggcg                                                                                                                                           >  1:167341/1‑151 (MQ=255)

        aTCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGc                                                                                                                                   <  2:112652/151‑1 (MQ=255)

        aTCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGc                                                                                                                                   <  1:340739/151‑1 (MQ=255)

                         cgatgcgatgCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTcg                                                                                                                  >  2:535841/1‑151 (MQ=255)

                           atgcgatgcgGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTcgcg                                                                                                                <  1:676330/151‑1 (MQ=255)

                                  gcggcgTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTa                                                                                                          <  1:80165/150‑1 (MQ=255)

                                    ggcgTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACg                                                                                                       >  2:150326/1‑151 (MQ=255)

                                        tttCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTc                                                                                                   >  2:320614/1‑151 (MQ=255)

                                         ttCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTc                                                                                                   >  1:630699/1‑150 (MQ=255)

                                            cATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATg                                                                                                >  2:265613/1‑150 (MQ=255)

                                               cAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAAcc                                                                                            >  2:138257/1‑151 (MQ=255)

                                                 aaCTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAAcc                                                                                            >  1:326808/1‑149 (MQ=255)

                                                    tGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGc                                                                                       >  1:733461/1‑151 (MQ=255)

                                                       catcaGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGAGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGc                                                                                    >  2:164142/1‑151 (MQ=255)

                                                               aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGc                                                                                    <  1:671004/143‑1 (MQ=255)

                                                               aGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGc                                                                                    >  2:670964/1‑143 (MQ=255)

                                                                   cGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGa                                                                         <  2:759655/150‑1 (MQ=255)

                                                                    gagaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGt                                                                       >  1:584760/1‑151 (MQ=255)

                                                                      gaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTg                                                                      <  2:639674/150‑1 (MQ=255)

                                                                      gaTTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGa                                                                     <  1:628429/151‑1 (MQ=255)

                                                                        ttCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATg                                                                   >  1:478869/1‑151 (MQ=255)

                                                                                     tGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGAttt                                                      >  1:130882/1‑151 (MQ=255)

                                                                                       aTTTCGGTCATATGACAAGCAATAAGGCTTTTGCGCGTTTAGTTATCCATTTCATGCGCGGGCGGGTGCAGCGGTGTTTCCAGTCCTCGTGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTtct                                                    >  2:766071/1‑151 (MQ=255)

                                                                                          tCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTcc                                                  <  2:608785/150‑1 (MQ=255)

                                                                                           cGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTg                                                >  2:57289/1‑151 (MQ=255)

                                                                                            ggTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGc                                               <  1:435168/151‑1 (MQ=255)

                                                                                               cATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAg                                             <  2:167334/150‑1 (MQ=255)

                                                                                               cATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAg                                             <  1:272451/150‑1 (MQ=255)

                                                                                                   tGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGgtgt                                         >  1:473775/1‑150 (MQ=255)

                                                                                                          cAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTc                                 >  2:34993/1‑151 (MQ=255)

                                                                                                           aaTAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                <  2:474861/151‑1 (MQ=255)

                                                                                                              aaGGCTTTTGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGcc                             <  2:630662/151‑1 (MQ=255)

                                                                                                                   ttttGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCg                        >  1:590258/1‑151 (MQ=255)

                                                                                                                    tttGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                <  2:472730/142‑1 (MQ=255)

                                                                                                                    tttGCGCGTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTcc                                >  1:472752/1‑142 (MQ=255)

                                                                                                                         gcgTTTCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTggggg                  >  1:142542/1‑151 (MQ=255)

                                                                                                                              tCGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCaaa              <  1:55871/150‑1 (MQ=255)

                                                                                                                               cGTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGt            <  2:664701/151‑1 (MQ=255)

                                                                                                                                gTTATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtt           <  2:650759/151‑1 (MQ=255)

                                                                                                                                 ttATCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGtt           >  2:360211/1‑150 (MQ=255)

                                                                                                                                   aTCCATTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGttatt        >  2:59677/1‑151 (MQ=255)

                                                                                                                                       aTTTCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGc    >  1:44486/1‑151 (MQ=255)

                                                                                                                                         ttCATGCGCGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTg  >  2:3817/1‑151 (MQ=255)

AAAATTTCATCGACATACATTTTAGCGATGCGATGCGGCGTTTCCATCAAACTGTCATCAGCCAGGTCGAGATTCAGCAGCTGCATGATTTCGGTCATATGACCAGCAATAAGGCTTTTGCGCGTTTCGTTATCCATTTCATGCACGGGCGGGCGCAGCGGTGTTTCCAGTCCTCGCGCAACTAACGCTTCATGAACCAGGGCCGCTTCTTTACTGAGTGATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTG  >  NC_000913/2243426‑2243713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A