Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,366,486 G→A A187T (GCT→ACT)  arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,366,4860GA100.0% 164.1 / NA 42A187T (GCT→ACT) arnBuridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

CCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACC  >  NC_000913/2366343‑2366622
                                                                                                                                               |                                                                                                                                        
ccATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAgg                                                                                                                                   <  1:274475/151‑1 (MQ=255)
  aTTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTg                                                                                                                                 <  1:75736/151‑1 (MQ=255)
    tCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTgg                                                                                                                                <  1:257322/150‑1 (MQ=255)
       cgcCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTg                                                                                                                            >  1:516684/1‑151 (MQ=255)
            ttGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGt                                                                                                                       >  1:764521/1‑151 (MQ=255)
                cGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACt                                                                                                                   <  2:83332/151‑1 (MQ=255)
                 gAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTA‑TACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGa                                                                                                                 <  1:731383/151‑1 (MQ=255)
                   aCGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGAt                                                                                                                <  1:640217/151‑1 (MQ=255)
                     gTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATaa                                                                                                              <  2:375549/151‑1 (MQ=255)
                           gCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGaaa                                                                                                         >  2:239046/1‑150 (MQ=255)
                               cGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCtt                                                                                                    >  1:687422/1‑151 (MQ=255)
                               cGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCtt                                                                                                    >  1:194960/1‑151 (MQ=255)
                                gCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTg                                                                                                   <  1:756453/151‑1 (MQ=255)
                                gCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTg                                                                                                   <  1:135943/151‑1 (MQ=255)
                                  aGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGcc                                                                                                 <  1:661058/151‑1 (MQ=255)
                                           aGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTa                                                                                        <  2:765188/151‑1 (MQ=255)
                                             aTGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACg                                                                                      <  2:764474/151‑1 (MQ=255)
                                              tgctgcCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACgg                                                                                     >  2:48983/1‑151 (MQ=255)
                                               gctgcCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACgg                                                                                     >  1:463623/1‑150 (MQ=255)
                                                        gCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGaaa                                                                            <  1:736684/150‑1 (MQ=255)
                                                        gCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAAt                                                                           >  2:197978/1‑151 (MQ=255)
                                                                 aCGTATTCCAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGt                                                                   <  2:94236/150‑1 (MQ=255)
                                                                     aTTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTGCGGATGCTGAAATTTCACGGTCTggg                                                              >  2:417359/1‑151 (MQ=255)
                                                                          aaaGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCg                                                         >  2:394386/1‑151 (MQ=255)
                                                                           aaGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGa                                                        <  1:399057/151‑1 (MQ=255)
                                                                               gCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGcc                                                    >  2:362861/1‑151 (MQ=255)
                                                                                 gACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCt                                                   >  1:308820/1‑150 (MQ=255)
                                                                                     tatTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATgac                                              >  1:680113/1‑151 (MQ=255)
                                                                                        tGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATgac                                              >  2:407635/1‑148 (MQ=255)
                                                                                                      ccGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGcc                              <  2:305275/150‑1 (MQ=255)
                                                                                                               tttcatttcaTGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGc                     >  1:472859/1‑150 (MQ=255)
                                                                                                                  catttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAgg                  <  1:239055/150‑1 (MQ=255)
                                                                                                                  catttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGc                 <  2:82519/151‑1 (MQ=255)
                                                                                                                   atttcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCt                >  1:550205/1‑151 (MQ=255)
                                                                                                                      tcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTg                           >  1:715154/1‑137 (MQ=255)
                                                                                                                      tcatGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTg                           <  2:715110/137‑1 (MQ=255)
                                                                                                                       catGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGaa             <  1:97414/150‑1 (MQ=255)
                                                                                                                       catGCCATTAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAg            <  2:266403/151‑1 (MQ=255)
                                                                                                                               tAAAAATATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAac     >  1:635475/1‑150 (MQ=255)
                                                                                                                                aaaaaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCt         >  2:156257/1‑145 (MQ=255)
                                                                                                                                aaaaaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCt         <  1:156264/145‑1 (MQ=255)
                                                                                                                                 aaaaTATTACCTGTACTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACAcc  >  2:588288/1‑151 (MQ=255)
                                                                                                                                               |                                                                                                                                        
CCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGATGCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCTATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACTGATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGATGCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACC  >  NC_000913/2366343‑2366622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A