Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,555,942	T→C	A153A (GCA→GCG)	eutL ←	putative ethanol utilization carboxysome structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,555,942	0	T	C	100.0%	177.9 / NA	50	A153A (GCA→GCG)	eutL	putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/26); total (24/26)

CTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAATATGCG  >  NC_000913/2555806‑2556087

ctTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGc                                                                                                                                     >  2:676483/1‑151 (MQ=255)

  ttACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGcc                                                                                                                                    <  1:274474/150‑1 (MQ=255)

      acGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCTATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCgg                                                                                                                               <  1:662116/151‑1 (MQ=255)

           gCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGc                                                                                                                          >  2:752146/1‑151 (MQ=255)

             cTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGcc                                                                                                                         >  1:197875/1‑150 (MQ=255)

                  tACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCg                                                                                                                    >  1:181533/1‑150 (MQ=255)

                    ccGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGa                                                                                                                 <  2:40343/151‑1 (MQ=255)

                      ggTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGTCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGAt                                                                                                                <  1:308235/150‑1 (MQ=255)

                      ggTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATc                                                                                                               <  2:409756/151‑1 (MQ=255)

                         tAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCcg                                                                                                            >  2:645094/1‑151 (MQ=255)

                         tAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCcg                                                                                                            >  2:538943/1‑151 (MQ=255)

                                gctgcCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTtg                                                                                                     >  1:234614/1‑151 (MQ=255)

                                   gcCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTtga                                                                                                    <  1:281744/149‑1 (MQ=255)

                                   gcCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTtga                                                                                                    <  2:597678/149‑1 (MQ=255)

                                    ccGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATCCGCCATCGGATCGCCAAGCGTGATCCCGGCGGGtgat                                                                                                   >  1:69027/1‑149 (MQ=255)

                                       agtagtTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATgagag                                                                                              <  1:617931/151‑1 (MQ=255)

                                          agtTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATa                                                                                           <  1:663148/151‑1 (MQ=255)

                                           gtTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATaa                                                                                          <  1:676578/151‑1 (MQ=255)

                                               gTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAAc                                                                                      <  1:216992/151‑1 (MQ=255)

                                                  tCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCg                                                                                    <  1:498278/150‑1 (MQ=255)

                                                  tCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCg                                                                                    <  2:50223/150‑1 (MQ=255)

                                                   cAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCgg                                                                                   >  1:600167/1‑150 (MQ=255)

                                                   cAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGt                                                                                  >  1:271949/1‑151 (MQ=255)

                                                     gACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCATCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTAc                                                                                <  2:645496/151‑1 (MQ=255)

                                                           ggCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGaa                                                                           <  1:5532/150‑1 (MQ=255)

                                                            gCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCTCGGCGGTTGTAAAGAGGAAAAAACCGGTACGCGAAAc                                                                         >  2:405626/1‑151 (MQ=255)

                                                              gggACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACt                                                                        >  2:377499/1‑150 (MQ=255)

                                                                 aCAGAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACa                                                                     <  2:156443/150‑1 (MQ=255)

                                                                   aTAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACAtgt                                                                  >  1:331631/1‑151 (MQ=255)

                                                                       gTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCa                                                              <  1:720009/151‑1 (MQ=255)

                                                                       gTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCa                                                              <  2:152600/151‑1 (MQ=255)

                                                                       gTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCa                                                              <  1:387977/151‑1 (MQ=255)

                                                                        tCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGcc                                                               >  2:481926/1‑149 (MQ=255)

                                                                         cGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAgg                                                            >  1:104483/1‑151 (MQ=255)

                                                                         cGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAgg                                                            >  1:748313/1‑151 (MQ=255)

                                                                           ccAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGaa                                                          <  2:240374/151‑1 (MQ=255)

                                                                                   aCGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTa                                                   <  2:364691/150‑1 (MQ=255)

                                                                                   aCGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTAt                                                  >  1:117983/1‑151 (MQ=255)

                                                                                     gTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCCGGAATGCCGGATc                                                 >  2:320889/1‑150 (MQ=255)

                                                                                        ggCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTgc                                             <  1:341825/151‑1 (MQ=255)

                                                                                         gCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTgcg                                            <  2:23251/151‑1 (MQ=255)

                                                                                                   gggCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGc                                  <  1:676524/151‑1 (MQ=255)

                                                                                                        gCATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACt                             >  1:674845/1‑151 (MQ=255)

                                                                                                         cATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGGTAAGAACCGGTACTCGGAACTACATGTGCCAGGAATGCTGTATCTTTCGCGTCATTAGCCCACTg                            >  1:12442/1‑151 (MQ=255)

                                                                                                         cATCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTg                            <  2:662896/151‑1 (MQ=255)

                                                                                                           tCAATGCCATAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTgg                           >  1:6169/1‑150 (MQ=255)

                                                                                                             aaTGCCTTAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGGGATAAGAACCGGTACGCGAAACTAAATTGGCAAGGTATTCCGTATCTTGCGCGTCCTTAACCCACTGGa                          >  1:418538/1‑149 (MQ=255)

                                                                                                                    tAGGTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCCAGCGCGATCCCGGCGGTTGATGGGAGAGAAGAACCGGGACGCGAAACTACATGTGCCAGGAATGCCGGATCTTGCGGGTCATTAACCCACTGGAAAGCTg                    >  2:49137/1‑148 (MQ=255)

                                                                                                                       gTCGCTTCCAGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCAt              <  1:741001/151‑1 (MQ=255)

                                                                                                                                aGCGGCGGCGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCCTTTTCAatat     >  2:435860/1‑151 (MQ=255)

                                                                                                                                 gcggcggcgCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAatat     <  1:509242/150‑1 (MQ=255)

                                                                                                                                   ggcggcGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAATATgcg  >  1:557417/1‑151 (MQ=255)

CTTTACACGCTGCCTGGCTACCGGTTAAAAATGCTGCCGAGTAGTTGGTTTCAGACGGTGGCGGGACATAGGTCGCCAGCTGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTACGCGAAACTACATGTGCCAGGAATGCCGTATCTTGCGCGTCATTAGCCCACTGGAAAGCTGCGCCATTTTCAATATGCG  >  NC_000913/2555806‑2556087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A