Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I1 R1	157	93.7	2947622	98.1%	2891617	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,476,595	A→G	pseudogene (12/15 nt)	pawZ →	tRNA‑OTHER

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,476,595	0	A	G	98.8%	312.6 / ‑3.7	85	pseudogene (12/15 nt)	pawZ	tRNA‑OTHER
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (43/41); total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

ATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTC  >  NC_000913/2476450‑2476741

aTGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATc                                                                                                                                               <  1:1317865/151‑1 (MQ=255)

  ggTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCt                                                                                                                                             >  2:661522/1‑151 (MQ=255)

  ggTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCt                                                                                                                                             >  1:1353956/1‑151 (MQ=255)

      atatCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTtgt                                                                                                                                          <  2:988768/150‑1 (MQ=255)

      atatCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTtgt                                                                                                                                          <  2:51566/150‑1 (MQ=255)

       tatCGTGATCATTGTGAATTTAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACAACATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTtgtg                                                                                                                                         <  1:197193/150‑1 (MQ=255)

        atCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGtt                                                                                                                                       >  1:761198/1‑151 (MQ=255)

           gTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTAt                                                                                                                                    <  2:856338/151‑1 (MQ=255)

             gACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATcc                                                                                                                                  >  1:274197/1‑151 (MQ=255)

              aCCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATcc                                                                                                                                  <  2:49013/150‑1 (MQ=255)

                cATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTa                                                                                                                               <  2:1284694/151‑1 (MQ=255)

                cATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTa                                                                                                                               <  1:497852/151‑1 (MQ=255)

                     tgAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAAcc                                                                                                                          >  2:72066/1‑151 (MQ=255)

                      gAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAAcc                                                                                                                          <  1:1344394/150‑1 (MQ=255)

                         ttCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGAAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACAt                                                                                                                      >  2:599424/1‑151 (MQ=255)

                          tCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACAt                                                                                                                      <  2:1215426/150‑1 (MQ=255)

                           cAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATaa                                                                                                                    <  2:380067/151‑1 (MQ=255)

                            aaaaaTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATaa                                                                                                                    <  2:1395274/150‑1 (MQ=255)

                             aaaaTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATaaaa                                                                                                                  <  2:498140/151‑1 (MQ=255)

                                aTAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAAcc                                                                                                               >  1:433392/1‑151 (MQ=255)

                                  aaGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGt                                                                                                             >  2:1453840/1‑151 (MQ=255)

                                   aGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGt                                                                                                             >  2:1299962/1‑150 (MQ=255)

                                     ctctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaa                                                                                                           >  2:871933/1‑150 (MQ=255)

                                     ctctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaa                                                                                                           >  1:106476/1‑150 (MQ=255)

                                      tctTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaat                                                                                                         >  2:466810/1‑151 (MQ=255)

                                         tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatta                                                                                                       >  1:1251546/1‑150 (MQ=255)

                                         tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatt                                                                                                        <  1:588632/149‑1 (MQ=255)

                                         tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaatt                                                                                                        <  1:1266373/149‑1 (MQ=255)

                                         tAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaat                                                                                                         <  1:206235/148‑1 (MQ=255)

                                          aaGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaattaa                                                                                                      >  2:1125023/1‑150 (MQ=255)

                                          aaGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGtaaattaa                                                                                                      >  1:808246/1‑150 (MQ=255)

                                               gcgcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGa                                                                                                <  2:1319951/151‑1 (MQ=255)

                                                cgcCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGaa                                                                                               <  1:14242/151‑1 (MQ=255)

                                                     aTGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGc                                                                                          >  2:486281/1‑151 (MQ=255)

                                                       gggTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCa                                                                                         <  1:401856/150‑1 (MQ=255)

                                                        ggTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAgg                                                                                       <  2:660440/151‑1 (MQ=255)

                                                         gTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGc                                                                                      >  1:1313642/1‑151 (MQ=255)

                                                         gTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGc                                                                                      <  1:230093/151‑1 (MQ=255)

                                                           aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGcc                                                                                     <  2:335962/150‑1 (MQ=255)

                                                           aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGcc                                                                                     <  2:922178/150‑1 (MQ=255)

                                                           aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg                                                                                    >  1:1059934/1‑151 (MQ=255)

                                                           aaaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg                                                                                    >  2:498399/1‑151 (MQ=255)

                                                            aaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCg                                                                                    >  1:203122/1‑150 (MQ=255)

                                                            aaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGa                                                                                   <  1:1166597/151‑1 (MQ=255)

                                                            aaaTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGa                                                                                   <  2:725724/151‑1 (MQ=255)

                                                              aTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAAt                                                                                 >  1:1240817/1‑151 (MQ=255)

                                                              aTAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAAt                                                                                 <  1:26039/151‑1 (MQ=255)

                                                                      tAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcac                                                                          >  1:1381159/1‑150 (MQ=255)

                                                                       aaaaTATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGcaca                                                                         <  2:591817/150‑1 (MQ=255)

                                                                         aaTATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACAcc                                                                       >  1:587935/1‑150 (MQ=255)

                                                                         aaTATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACAcc                                                                       >  1:206937/1‑150 (MQ=255)

                                                                                  cacaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACt                                                                <  1:991777/148‑1 (MQ=255)

                                                                                  cacaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCg                                                              >  2:1063943/1‑150 (MQ=255)

                                                                                  cacaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGt                                                             >  1:870267/1‑151 (MQ=255)

                                                                                   acaTCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTg                                                            >  1:803681/1‑151 (MQ=255)

                                                                                         aaaaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGtttt                                                       >  2:145702/1‑150 (MQ=255)

                                                                                           aaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAtt                                                    >  1:425794/1‑151 (MQ=255)

                                                                                           aaaaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTAtt                                                    >  2:833502/1‑151 (MQ=255)

                                                                                             aaCACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTc                                                   >  2:584672/1‑150 (MQ=255)

                                                                                                 ccATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTa                                               <  2:702838/150‑1 (MQ=255)

                                                                                                 ccATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTAt                                              <  1:268997/151‑1 (MQ=255)

                                                                                                    ttCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGa                                            >  2:853948/1‑150 (MQ=255)

                                                                                                       caatcaatCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTg                                        >  1:812543/1‑151 (MQ=255)

                                                                                                        aatcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTgg                                       >  1:1252132/1‑151 (MQ=255)

                                                                                                        aatcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTgg                                       >  2:1009464/1‑151 (MQ=255)

                                                                                                          tcaatcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGtt                                     >  1:930796/1‑151 (MQ=255)

                                                                                                             atcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGttttt                                  >  2:374217/1‑151 (MQ=255)

                                                                                                              tcCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGttttt                                  >  1:608653/1‑150 (MQ=255)

                                                                                                               ccccTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTa                                 <  2:1353850/150‑1 (MQ=255)

                                                                                                               ccccTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTAc                                >  2:1053797/1‑151 (MQ=255)

                                                                                                                cccTGCTGCTTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTAcc                               >  2:1368539/1‑151 (MQ=255)

                                                                                                                       gctTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTtctc                        >  1:614382/1‑151 (MQ=255)

                                                                                                                       gctTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTtctc                        <  1:661595/151‑1 (MQ=255)

                                                                                                                       gctTCAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTtct                         <  2:1235051/150‑1 (MQ=255)

                                                                                                                           cAAGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAg                    <  2:936281/151‑1 (MQ=255)

                                                                                                                             aGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGcc                  <  2:8360/151‑1 (MQ=255)

                                                                                                                             aGTAGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGcc                  <  2:500736/151‑1 (MQ=255)

                                                                                                                               tAGATGTCTGCAGGGGACGCCATCCTTGTTTTTATCCCTAAAACCACATAAAAACCGAAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGc                   >  1:256873/1‑148 (MQ=255)

                                                                                                                                aGATGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGa               <  2:331748/151‑1 (MQ=255)

                                                                                                                                  aTGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGata             <  2:1137798/151‑1 (MQ=255)

                                                                                                                                   tGTCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGata             >  1:1250606/1‑150 (MQ=255)

                                                                                                                                     tCTGCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGataat           >  1:326245/1‑150 (MQ=255)

                                                                                                                                        gCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGataata          <  1:790942/148‑1 (MQ=255)

                                                                                                                                        gCAGGGGACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGataata          <  2:1398364/148‑1 (MQ=255)

                                                                                                                                             ggACGCCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTc  <  2:1109989/151‑1 (MQ=255)

ATGGTTATATCGTGACCATTGTGAATTCAAAAATAAGCTCTTAAGCCGCGCCAATGGGTAAAATAGCGGGTAAAATATTTCTCACATCTAAAAAACACCATTCCAATCAATCCCCTGCTGCTTCAAGTAGATGTCTGCAGGGGACACCATCCTTGTGTTTATCCCTAAAACCACATAAAAACCGTAAATTAAATTCGAACTAGCAGGCCGAATAAGGCACACCAGGAACTCGTGGTTTTATTCATTATGAAGTGGTTTTTACCTGTTCTCTTAGCCAGATAATAAACGCTTC  >  NC_000913/2476450‑2476741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A